Diversity and Distribution of Dematiaceous Fungi in Çamaltı Saltern in İzmir Province, Turkey

Year 2020, Volume: 11 Issue: 1, 29 - 39, 27.04.2020

Özden Özgök Semra İlhan

Abstract

Dematiaceous fungi (black fungi) are a heterogeneous group of fungi present in diverse environments worldwide. Many species in this group are known for surviving in extreme conditions, especially in tropical and subtropical climates. In this study, a total of 16 water samples which were obtained seasonally from 4 different saltpans predetermined in the Çamaltı Saltern were studied. The isolation of fungi was performed by membrane filtration method using DRBC and DRBC10 media. The isolated fungi were identified based on their morphological characteristics and the identification was supported by internal transcribed spacer (ITS)-based phylogenetic analysis.
The average numbers of colonies were determined to be 15 CFU / 100 ml and 24 CFU / 100 ml on DRBC and DRBC10 media, respectively. Thirty species of 10 genera were identified. The genera are as follows: Alternaria, Arthrinium, Biscogniauxia, Chaetomium, Cladosporium, Drechslera, Phomopsis, Pithomyces, Stachybotrys and Stemphylium. According to their relative abundance, the dominant genera isolated on DRBC medium were Cladosporium (52%), Alternaria (35%) and Chaetomium (6%), while Alternaria (50%) and Cladosporium (47%) on the DRBC10 medium. The fungi isolated from Çamaltı Saltern water samples were found to belong mainly to Capnodiales and Pleosporales. This study represents the first survey of dematiaceous fungi in İzmir Çamaltı Saltern and provides data on their diversity and distribution.

Keywords

Solar Saltern, Hypersaline, Black Fungi, Cladosporium, Alternaria

Supporting Institution

ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ

Project Number

201319A101

Thanks

This study was supported by Scientific Research Projects Commission of Eskişehir Osmangazi University with a Project number of 201319A101.

İzmir İlinde Bulunan Çamaltı Tuzlası Dematiaceous Fungus Çeşitliliği ve Dağılımı

Abstract

Dematiaceous funguslar (siyah funguslar) tüm dünyada çeşitli ortamlarda bulunan heterojen bir mantar grubudur. Bu gruptaki birçok türün, özellikle tropikal ve subtropikal iklimlerde, ekstrem koşullarda yaşadığı bilinmektedir. Bu çalışmada, Çamaltı tuzlasında belirlenen 4 farklı havuzdan mevsimsel olarak alınan toplam 16 su örneği çalışılmıştır. Mikrofungusların izolasyonu DRBC ve DRBC10 besiyerlerinde membran filtrasyon yöntemi ile gerçekleştirilmiştir. İzole edilen mikrofunguslar morfolojik özellikleri esas alınarak tanımlanmış ve ITS’ye dayalı filogenetik analiz ile desteklenmiştir.
Ortalama koloni sayısı DRBC besiyerinde 15 KOB / 100 ml ve DRBC10 besiyerinde 24 KOB / 100 ml bulunmuştur. Sonuç olarak, 10 cinse ait 30 tür belirlenmiştir. Bu cinsler; Alternaria, Arthrinium, Biscogniauxia, Chaetomium, Cladosporium, Drechslera, Phomopsis, Pithomyces, Stachybotrys ve Stemphylium’dur. Göreceli bolluklarına göre, izole edilen baskın cinsler DRBC besiyerinde Cladosporium (%52), Alternaria (%35) ve Chaetomium (%6) iken DRBC10 besiyerinde Alternaria (%50) ve Cladosporium (%47) olarak belirlenmiştir. Çamaltı Tuzlası tuzlu su örneklerinden izole edilen mikrofungusların ağırlıklı olarak Capnodiales ve Pleosporales takımlarına ait olduğu görülmüştür. Bu çalışma İzmir Çamaltı Tuzlasındaki Dematiaceous fungus çeşitliliği üzerine yapılan ilk çalışmayı temsil etmektedir.

Keywords

Güneş Tuzlası, Hipersalin, Siyah Funguslar, Cladosporium, Alternaria

Project Number

201319A101

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