Acıgöl/Türkiye’deki Haloalkalitolerant ve Haloalkalifilik Fungus Çeşitliliği

Year 2021, Volume: 12 Issue: 1, 33 - 41, 30.04.2021

Fatma Ayva Rasime Demirel Semra İlhan Lira Usakbek Kyzy Uğur Çiğdem Niyazi Can Zorluer Emine İrdem Ercan Özbiçen Esma Ocak Gamze Tunca

Abstract

Mikrofunguslar, çevrede en yayın olarak bulunan mikroorganizmalardır. Bu organizmalar, organik maddelerin ayrıştırıcısı, endüstriyel ve gıda alanlarında bazı ürünlerin üreticisi, önemli mikotoksinlerin, bitki, hayvan ve insan yaşamı üzerindeki önemli ekonomik ve sağlık etkilerinin nedeni olarak bilinirler. Bu nedenlerden dolayı mikrofungusların biyolojik çeşitliliğinin belirlenmesine yönelik çalışmalar hayati önem taşımaktadır. Bu çalışmanın amacı, dünyanın ikinci büyük alkali gölü olan Acıgöl Gölü'ndeki mikrofungusların biyolojik çeşitliliğinin araştırılmasıdır.
Bu amaçla, su örneği Kasım 2019'da Acıgöl Gölü'ndeki tuzladan kompozit olarak alınmıştır. Su örneği pH ve tuzluluk açısından analiz edilmiştir. Mantar türlerinin sudan izole edilmesi ve sayılması için filtrasyon yöntemi ve farklı DRCB besi ortamları kullanılmıştır. İzolatların tuz tolerans aralığı belirlenmiştir.
DRBC ve DRBC28 ortamlarından sırasıyla toplam 260 KOB/L ve 65 KOB/L sayılmıştır. Saflaştırma adımlarından sonra, toplam 52 izolat elde edilmiştir ve geleneksel yöntemler ve çoklu lokus genleri dizilimi kullanılarak izolatlar tanımlanmıştır.
Sonuçlar, Acıgöl Gölü bölgesinin sırasıyla Aspergillus (% 25) ve Penicillium (% 27) cinsleri bakımından zengin olduğunu göstermiştir. Bölgede cinsin diğer üyeleri belirlenmekle birlikte toplam diğer üyeler % 48 oranında bulunmuştur. Ayrıca Cladosporium acalypha ve Penicillium sizovae Türkiye için yeni kayıt olarak belirlenmiştir. Bu çalışma ile belirlenen mikrofungus biyoçeşitliliğinin toksin üretme kabiliyetine sahip olması, patojenik ve saprofit türleri içermesi, halk sağlığı için dikkate alınması gereken bir konu olarak belirlenmiştir.

Keywords

Acıgöl, Göl, Mikrofungus, Biyoçeşitlilik

Haloalkalitolerant and Haloalkaliphilic Fungal Diversity of Acıgöl/Turkey

Abstract

Microfungi are the most common microorganisms found in range from environment. They are well known as producer of some product in industrial and food fields, decomposer of organic matter, of important mycotoxins, reason major economic and health effects on plant, animal and human life. Because of these reasons, studies on the determination of biodiversity of microfungi are vitally important. Aim of this study is investigation of biodiversity of microfungi in Acıgöl Lake that is the second largest alkaline lake in the world.
For this purpose, the water sample was compositely taken from a saltern of Acıgöl Lake in November 2019. The samples have been analysed in terms of pH, and salinity. To isolate and enumerate the fungal species from water, filtration method and different DRBC medium types were used. Salt tolerance range of isolates were determinated.
A total of 260 CFUs/L and 65 CFUs/L were counted from DRBC and DRBC28 media, respectively. After purification steps, totally 52 isolates were obtained and identified by using conventional methods and multi locus genes sequencing.
The results indicated that the Acıgöl Lake Region has rich for Aspergillus (25%) and Penicillium (27%) genera, respectively. Although other members of the genus were determined in the region, other members were found to be 48% in total. In addition, Cladosporium acalypha and Penicillium sizovae was determined as a new recorded for Turkey. The fact that the microfungus biodiversity determined by this study has the ability to produce toxins (such as Aspergillus flavus), contains pathogenic (such as plant pathogen Fusarium genus members) and saprophyte species, has been identified as an issue to be considered for public health.

Keywords

Acıgöl, Lake, Microfungus, Biodiversity

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