Assessment of Cytotoxic Effect of Porodaedalea pini Mushroom on Prostate Cancer

Year 2022, Volume: 13 Issue: 2, 112 - 119, 31.10.2022

Ebru Deveci , Gülsen Tel-çayan , Mehmet Emin Duru

Abstract

Phellinus species are in the classification of valuable mushrooms among medicinal mushrooms and have been the source of traditional medicine for centuries. In this study, the hexane (PPH), methanol (PPM) and water (PPW) extracts, fractions and isolated compounds from Porodaedalea pini (Çamkavı mantarı) were screened for their cytotoxic effects on PC3 (prostate cancer) and 3T3 (murine fibroblast) cell lines by MTT assay. PPH was found to have moderate cytotoxicity on PC3 cell with the IC50 values of 33.84±0.01 µg/mL. The fatty acid profile of PPH was identified by GC-FID (gas chromatography-flame ionization dedector) and GC-MS (gas chromatography-mass spectrometry) and the main fatty acids were recorded as palmitic (41.40±0.03%), linoleic (21.53±0.01%), oleic (12.06±0.01%), and stearic (8.20±0.01%) acids. PPM and PPW were fractioned by using liquid-liquid extractions. Among all fractions, the 2-butanol fraction of the methanol extract (PPM-B) indicated the best cytotoxicity on PC3 and 3T3 cell lines. Also, among the isolated compounds (dioctyl phthalate (1), ergosterol peroxide (2) and pinoresinol (3)) from PPM, ergosterol peroxide (2) was found as moderate cytotoxic on PC3 with the IC50 value of 95.47±1.01 µg/mL. This study, in which the effect of P. pini on PC3 cell was examined for the first time, proved its medicinal importance by revealing the cytotoxic properties of the species.

Keywords

Porodaedalea pini, Prostate cancer, Extract, Fatty acid, Isolation

Porodaedalea pini Mantarının Prostat Kanseri Üzerindeki Sitotoksik Etkisinin Değerlendirilmesi

Abstract

Phellinus türleri şifalı mantarlar arasındaki değerli mantarlar sınıfında yer almaktadır ve yüzyıllardır geleneksel tıbbın kaynağı olmuştur. Bu çalışmada, Porodaedalea pini (Çamkavı mantarı)'den elde edilen hekzan (PPH), metanol (PPM) ve su (PPW) ekstrelerinin, fraksiyonlarının ve izole edilmiş bileşiklerin PC3 (prostat kanseri) ve 3T3 (fare fibroblast) hücre hatları üzerindeki sitotoksik etkileri MTT testi kullanılarak taranmıştır. PPH’ın 33.84±0.01 µg/mL olan IC50 değeri ile PC3 üzerinde orta derecede sitotoksisiteye sahip olduğu bulunmuştur. PPH’ın yağ asidi profili GC-FID (gaz kromatografisi-alev iyonizasyon dedektörü) ve GC-MS (gaz kromatografisi-kütle spektrometresi) ile belirlenmiş ve majör yağ asitleri palmitik (%41.40±0.03), linoleik (%21.53±0.01), oleik (%12.06±0.01) ve stearik (%8.20±0.01) asit olarak kaydedilmiştir. PPM ve PPW ekstreleri, sıvı-sıvı ekstraksiyon tekniği kullanılarak fraksiyonlandırılmıştır. Tüm fraksiyonlar arasında metanol ekstresinden elde edilen 2-bütanol fraksiyonu (PPM-B) PC3 ve 3T3 hücre hatlarında en iyi sitotoksisiteyi göstermiştir. Ayrıca, PPM’den izole edilen bileşiklerden (dioktil ftalat (1), ergosterol peroksit (2) ve pinoresinol (3)), ergosterol peroksit (2) 95.47±1.01 µg/mL olan IC50 değeri ile PC3 hücre hattında orta derecede sitotoksik olarak bulunmuştur. P. pini'nin prostat kanseri üzerindeki etkisinin ilk kez incelendiği bu çalışmada, türün sitotoksik özellikleri ortaya çıkartılarak tıbbi önemi kanıtlanmıştır.

Keywords

Porodaedalea pini, Prostat kanseri, Ekstre, Yağ asidi, İzolasyon

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