Türkiye'den Melanoleuca cinereifolia Türünün Moleküler ve Morfolojik Teşhisi

Year 2022, Volume: 13 Issue: 2, 134 - 140, 31.10.2022

Ayşenur Kalmer , Ayten Dizkırıcı , İsmail Acar

Abstract

Bu çalışmada Melanoleuca cinereifolia, Türkiye'nin Diyarbakır ilinden ilk kez rapor edilmiş, makro/mikro-morfolojik karakterleri ile nükleer ribozomal Internal Transcribed Spacer (nrITS) bölgesinin DNA dizileri baz alınarak tanımlanmıştır. Türün makro ve mikro morfolojik tanımı, doğal ortamdaki taze bazidyomların fotoğrafları ve mikroskobik yapılarının çizimleri ile birlikte verilmiştir. Bu tür esas olarak kısa saplı, grimsi kahverengi şapka, gri lamelli ve şişe biçiminde sistityumlu bazidiyom ile karakterize edilir. Filogramda, M. cinereifolia, Melanoleuca alt cinsindeki akrabaları ile birlikte kümelenmiştir. Türler ve yakın akrabaları arasındaki morfolojik ve moleküler benzerlikler ve farklılıklar ayrıntılı olarak tartışılmıştır.

Keywords

DNA, ITS, Moleküler taksonomi, Agaricales

Molecular and Morphological Identification of Melanoleuca cinereifolia from Türkiye

Abstract

Melanoleuca cinereifolia is identified based on macro/micro-morphological characters and DNA sequences of the nuclear ribosomal Internal Transcribed Spacer (nrITS) region and is reported here for the first time from Diyarbakır province of Türkiye. Macro- and micro-morphological description of the species is provided along with the photographs of fresh basidiomes in natural habitat and the line drawings. The species is mainly characterized by basidiome with a short stipe, grayish-brown pileus, grey lamellae and lageniform cystidia. In the phylogram, M. cinereifolia is clustered with its allies in the subgenus Melanoleuca. Morphological and molecular similarities and differences among the species and the close relatives were discussed in detail.

Keywords

DNA, ITS, Molecular taxonomy, Agaricales

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