Phenotypic and Molecular Identification of Green Pea Powdery Mildew Pathogen from Alborz, Iran

Year 2022, Volume: 13 Issue: 3, 59 - 65, 30.12.2022

Simin Taheri Ardestani , Aliakbar Khodaparast , Ahmad Abbasi Moghadam , Mehrzad Ahmadi

https://doi.org/10.30708/mantar.1187178

Abstract

Green pea (Pisum sativum.) is an important consumable vegetable crop worldwide rich in protein, vitamins, and nutrition. It is cultivated in many parts of Iran, particularly during the summer season, and recently under protected greenhouse. Powdery mildew is an important disease of green pea, especially during the pod fill and maturation stage and decreases the photosynthetic area of leaves which causes crop loss. This experiment was conducted in order to identify fungi associated with powdery mildews on some accessions under regeneration of green pea collection at the genetic department and national plant gene bank of Iran, seed, and plant improvement institute during the year 2021. Powdery mildew became epidemic on the plants at the pod stage from the end of July to August. The formation of white, floury patches initially on the leaf progressing towards tendrils, pods, and stems observed that on susceptible accessions covered the most aerial part of the plants. The Erysiphe pisi, Erysiphe trifolii and Erysiphe baeumleri were reported causing disease on pea. The infected plant samples were collected and transferred to the lab for morphological studies, then, the genomic DNA was isolated and used for amplification and sequencing using the internal transcribed spacers (ITS) regions. Based on morphological characteristics of asexual and sexual stages as well as rDNA-ITS sequence analyses, the fungus was identified as Erysiphe pisi. Due to the presence of accessions with high levels of resistance to this disease, further investigation is recommended to breed resistant cultivars for cultivation in this province.

Keywords

Pisum sativum, Erysiphe pisi, rDNA, ITS sequence analyses

Supporting Institution

Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO)College of Agricultural Sciences, University of Guilan

Thanks

The authors wish to thanks the Seed and Plant Improvements Institue, Agrisultural research, education and extension organization of Iran and the university of Guilan that provides facility for the conducting the experiments.

Alborz, İran'dan Yeşil Bezelye Külleme Patojeninin Fenotipik ve Moleküler Tanımlanması

Abstract

Bezelye (Pisum sativum.), dünya çapında protein, vitamin ve besin açısından zengin, önemli bir tüketilebilir sebze ürünüdür. İran'ın birçok yerinde özellikle yaz mevsiminde ve son zamanlarda örtülü seralarda yetiştirilmektedir. Külleme, yeşil bezelyenin özellikle bakla doldurma ve olgunlaşma aşamasında önemli bir hastalığıdır ve yaprakların fotosentetik alanını azaltarak ürün kaybına neden olur. Bu deney, 2021 yılında İran genetik departmanı ve ulusal bitki gen bankası, tohum ve bitki ıslah enstitüsünde yeşil bezelye koleksiyonunun rejenerasyonu altındaki bazı çeşitlerde külleme ile ilişkili mantarları belirlemek için yapılmıştır. Temmuz sonundan ağustos ayına kadar bitkiler bakla aşamasındadır. Başlangıçta yaprak üzerinde dallara, baklalara ve gövdelere doğru ilerleyen beyaz, unlu yamaların oluşumu, duyarlı erişimlerde bitkilerin en hava kısmını kapsadığı gözlendi. Erysiphe pisi, Erysiphe trifolii ve Erysiphe baeumleri'nin bezelyede hastalığa neden olduğu bildirilmiştir. Enfekte bitki örnekleri toplandı ve morfolojik çalışmalar için laboratuvara aktarıldı, ardından genomik DNA izole edildi ve dahili transkripsiyonlu ayırıcılar (ITS) bölgeleri kullanılarak amplifikasyon ve sekanslama için kullanıldı. Eşeysiz ve eşeyli evrelerin morfolojik özelliklerine ve ayrıca rDNA-ITS sekans analizlerine dayanarak, mantar Erysiphe pisi olarak tanımlandı. Bu hastalığa karşı yüksek düzeyde dirençli çeşitlerin varlığından dolayı, bu ilde dayanıklı çeşitler üretmek için daha fazla araştırma yapılması önerilir.

Keywords

Pisum sativum, Erysiphe pisi, rDNA, ITS dizi analizleri

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