A mixed Bacillus gibsonii and Sphingomonas echinoides infection in cultured rainbow trout (Oncorhynchus mykiss)

Year 2021, , 71 - 79, 31.12.2021

Özgür Çanak , Tülay Akaylı , Çiğdem Ürkü

https://doi.org/10.51756/marlife.999539

Cited By: 2

Abstract

Rainbow trout (Oncorhynchus mykiss) is a fish species with a long history of cultivation and bacterial pathogens are limiting the success rate. The aim of this study is the biochemical and molecular identification of two opportunistic pathogens detected in the rainbow trout cultured in net cages in a dam lake located on the Kızılırmak river; revealing the pathological symptoms of them in the moribund fish samples; determination of their antimicrobial susceptibility profile and determination of the antagonistic effect of two probiotic-candidate strains against them. Depending on the results of the conventional bacteriologic and molecular identification studies, bacterial isolates recovered from the internal organs of the moribund fish samples, a mixed bacterial infection case of Bacillus gibsonii and Sphingomonas echinoides was identified in the moribund fish samples showing general bacterial hemorrhagic septicemia symptoms for the first time in rainbow trout. Despite it was not possible to identify these isolates at the species level using conventional bacteriological methods, our isolates separately showed similarities more than 99% with the above mentioned species in the 16s RNA sequence analysis. The results of this study showed that, long term water quality parameter determination and bacterial distribution monitoring studies which include molecular tools should be carried out in the aquaculture sites to increase the success in trout culture.

Keywords

aquaculture, Fish diseases, Rainbow trout, Bacillus gibsonii, Sphingomonas echinoides

Supporting Institution

İstanbul Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

FBA-28476

Thanks

This study was supported by Istanbul University Research Projects coordination unit with the project number: FBA-2018-28476. The authors would like to thank other staff of the project, Dr. Eda YARDIMCI and Dilek OKMEN who were not added to the author list of this manuscript with their own request.The authors also would like to thank the staff of the fish farms for their valuable helps during field sampling.

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