Ergene Nehir Suyunun Soğan (Allium cepa L.) Kök Ucu Hücrelerinde Sitogenetik Etkisinin Değerlendirilmesi

Year 2017, Volume: 29 Issue: 3, 111 - 117, 01.11.2017

Martin Kanev , Kezban Yıldırım Fulya Dilek Gökalp

https://doi.org/10.7240/marufbd.311079

Abstract

Türkiye’de
endüstriyel hareketlerin artması ile birlikte, Trakya bölgesinde organize
sanayi alanları kurulmuş ve bunun sonucunda özellikle Trakya bölgesini besleyen
Ergene Nehri ve çevre alanlarında kirlilik artmıştır. Bu çalışmada, Ergene
nehir suyunun Allium cepa L. kök ucu
hücrelerinde genotoksik ve sitotoksik etkilerinin araştırılması amaçlanmıştır.
Çalışmada Ergene nehir suyundan iki farklı istasyondan aynı dönemde alınan su
örnekleri 0.1/100 mL, 1/100 mL, 5/100 mL, 10/100 mL, 20/100 mL ve 40/100 mL konsantrasyonlar
ile soğan (Allium cepa L.) kök ucu
hücreleri 24 saat muamele edilerek sitogenetik anormallikler ve mitotik indeks
saptanmıştır. Her iki istasyondan alınan örneklerin, uygulanan tüm dozlarında
mitotik anormallikleri kontrole göre anlamlı bir şekilde arttırdığı ve mitotik
indeksi anlamlı olarak değiştirdiği belirlenmiştir. En fazla gözlenen mitotik
anormallikler kromozomlarda asenkronizasyon, yanlış kutuplaşma ve C-mitozdur.
Sonuçlar Ergene nehir suyunun A. cepa
testinde genotoksik ve sitotoksik etkili olduğunu göstermektedir.

Keywords

Ergene Nehri, Allium cepa, sitotoksik, genotoksik

Evaluation Of Cytogenetic Effects Of Ergene River Water On Onion (Allium cepa L.) Root Tip Cell

Abstract

As a result of industrialization movement in Turkey,
organized industrial zone have been 
established in Thrace, and especially pollution reached significant size
in there and their surrounding area. In the present study, it was aimed to
study the genotoxic and cytotoxic effects of the Ergene River water on Allium cepa root tip cells. In the
study, Ergene river water samples taken at the same time from two different
stations. Samples were exposed with 0.1/100 mL, 1/100 mL, 5/100 mL, 10/100 mL,
20/100 mL, and 40/100 mL doses for 24 hours on onion (Allium cepa L.) root tip cells, and cytogenetic abnormalities and
mitotoic index were investigated. On applied all doses of both stations,
mitotic abnormalities were significantly increased compared with control, and
mitotic index was significantly changed. The most observed mitotic
abnormalities in chromosomes are asencronization, incorrect polarization, and
C-mitosis. The results indicated that the Ergene River has genotoxic, and
cytotoxic effects on plants.

Keywords

The Ergene River, Allium cepa, cytotoxic, genotoxic

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