Hücre Tipine Özgü Analiz : Arabidopsis thaliana Yapraklarında Floresans ile Aktive Edilmiş Hücrelerin Toplanması Yönteminin Kullanımı
Abstract
Gen aktivitesinin hücreye özgü
çözümü özel gelişimsel olayların anlaşılmasında kritik bir rol oynamaktadır.
Bunun yanında, genomik seviyede böyle yüksek çözünürlüklü verilerin elde
edilmesi spesifik hücrelerin izole edilmesini gerektirdiği için oldukça zordur.
Aynı organ ya da dokunun komşu hücrelerinden spesifik hücrelerin izole
edilebilmesi için en etkili yöntem FACS (Fluorescence-activated cell sorting=Floresans
tarafından aktive edilmiş hücrelerin ayrılması) yöntemidir. İzole edilen
hücreler genomik analizler için kullanılabilmektedir. Bu derlemede
GFP (Green flourescent protein=Yeşil floresans protein) ile işaretlenmiş
transgenik Arabidopsis thaliana
bitkilerinin yapraklarında FACS uygulaması hakkında bilgi verilmiş olacaktır.
Keywords
References
- Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Galbraith D.W., Benfey P.N., 2003, A gene expression map of the Arabidopsis root. Science, 302:1956-1960
- Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Hirst, J.A., Galbraith D.W., Benfey P.N., 2005, Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent repeater lines. Nat. Methods., 2:615-619
- Carter A.D., Bonyadi R., Gifford M.L., 2013, The use of fluorescence-activated cell sorting in studying plant development and environmental responses. Int. J. Dev. Biol., 57:545-552
- Gronlund J.T., Eyres A., Kumar S., Buchanan-Wollaston V., Gifford M.L., 2012, Cell specific analysis of Arabidopsis leaves using flourescence activated cell sorting, J. Vis. Exp. (68), e4214 10.3791/4214 DOI: 10.37914214 (2012)
- Hertberg M., Sievertzaon M., Aspeborg H., Nilsson P., Sandberg G., Lundeberg J., cDNA microarray analysis of small plant tissue samples using cDNA target amplification protocol. Plant J., 25:585-591
- Lee J.Y., Levesque M., Benfey P.N., 2005, High-throughput RNA isolation technologies. New tools for high-resolution gene expression profiling in plant systems. Plant Physiol., 138:585-590
- Wang Y. and Jiao Y., 2011, Advances in plant cell type-spesific genome-wide studies of gene expression. Front. Biol., 6:384-389
Cell-type specific analysis: The use of fluorescence-activated cell sorting (FACS) in Arabidopsis thaliana leaves
Abstract
Cell-specific resolution of gene activity is
critical to understand specific developmental events. However, such high
resolution data have been difficult to obtain at a genomic level because
specific types of cell need to be isolated. One of the most efficient method to
isolate specific cells from neighboring cells in the same organ or tissue is
FACS. The
isolated cells can be used for genomic analysis. In this review, it will be
given information about FACS in transgenic Arabidopsis
thaliana leaves which are labelled
with GFP.
Keywords
References
- Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Galbraith D.W., Benfey P.N., 2003, A gene expression map of the Arabidopsis root. Science, 302:1956-1960
- Birnbaum K., Jung J.W., Wang J.Y., Lambert G.M., Hirst, J.A., Galbraith D.W., Benfey P.N., 2005, Cell type-specific expression profiling in plants via cell sorting of protoplasts from fluorescent repeater lines. Nat. Methods., 2:615-619
- Carter A.D., Bonyadi R., Gifford M.L., 2013, The use of fluorescence-activated cell sorting in studying plant development and environmental responses. Int. J. Dev. Biol., 57:545-552
- Gronlund J.T., Eyres A., Kumar S., Buchanan-Wollaston V., Gifford M.L., 2012, Cell specific analysis of Arabidopsis leaves using flourescence activated cell sorting, J. Vis. Exp. (68), e4214 10.3791/4214 DOI: 10.37914214 (2012)
- Hertberg M., Sievertzaon M., Aspeborg H., Nilsson P., Sandberg G., Lundeberg J., cDNA microarray analysis of small plant tissue samples using cDNA target amplification protocol. Plant J., 25:585-591
- Lee J.Y., Levesque M., Benfey P.N., 2005, High-throughput RNA isolation technologies. New tools for high-resolution gene expression profiling in plant systems. Plant Physiol., 138:585-590
- Wang Y. and Jiao Y., 2011, Advances in plant cell type-spesific genome-wide studies of gene expression. Front. Biol., 6:384-389
Details
| Subjects | Engineering |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | December 31, 2017 |
| Acceptance Date | December 19, 2017 |
| Published in Issue | Year 2017 Volume: 29 Issue: 4 |
Cite
Marmara Fen Bilimleri Dergisi
e-ISSN : 2146-5150
MU Fen Bilimleri Enstitüsü
Göztepe Yerleşkesi, 34722 Kadıköy, İstanbul
E-posta: fbedergi@marmara.edu.tr