Carpobrotus acınaiformis L. Metanol Ekstresinin Fitokimyasal Taraması ve Ekstrenin İnsan Metastatik Meme Kanseri (MCF-7) ve İnsan Kolon Kanseri (Caco-2) Hücreleri Üzerindeki Sitotoksik ve Apoptotik Etkisinin Araştırılması

Year 2018, Volume: 30 Issue: 2, 163 - 175, 30.06.2018

Tülay Aşkın Çelik , Özlem Sultan Aslantürk

https://doi.org/10.7240/marufbd.339484

Cited By: 2

Abstract

Bu çalışmanın amacı, C. acinaciformis L. (makas
otu) bitkisinin toprak üstü kısımlarından elde edilen metanol ekstresinde (ME)
fitokimyasal madde taramasının yapılması, ekstrenin toplam fenolik madde
miktarı, antioksidan aktivitesi ve in
vitro sitotoksik ve apoptotik etkilerinin araştırılmasıdır. Ekstrede
bulunan fitokimyasallar standart metod ve HPLC analizi kullanılarak belirlenmiştir.
C. acinaciformis ME, antioksidan
aktivitesi (DPPH radikalini süpürme, H₂O₂ süpürme
and metal şelatlama assay), sitotoksik etki (MCF-7 ve Caco-2 hücrelerinde MTT
assay) ve apoptotik etki (DNA difüzyon assay) bakımından test edilmiştir.

Yapılan kalitatif fitokimyasal tarama test sonuçlarına göre C. acinaciformiş ME’de fenoller,
taninler, flavonoidler ve antrakinon’ların bulunduğu, alkaloid ve saponinin ise
bulunmadığı belirlenmiştir. Ekstrenin toplam fenolik madde miktarı 61.26 ±
0,110 mg GAE/g ekstre olarak hesaplanmıştır. ME’nin denenen bütün
konsantrasyonlarda (100 μg/ml, 250 μg/ml, 500 μg/ml ve 1000 μg/ml) yüksek
seviyede DPPH radikalini süpürme aktivitesine sahip olduğu H₂O₂
süpürme aktivitesinin çok düşük olduğu,  metal şelatlama aktivitesinin ise bulunmadığı belirlenmiştir.

ME, MCF-7 hücreleri
üzerinde oldukça yüksek oranda sitotoksik etki göstermiş (p<0.05), Caco-2 hücreleri
üzerindeki sitotoksik etkisi ise daha düşük oranda olmuştur. ME’nin MCF-7 ve
Caco-2 hücreleri üzerinde oluşturduğu apoptotik etki de sitotoksik etkiye
benzerlik göstemiştir. Metanol ekstresinin MCF-7 hücreleri üzerindeki apoptotik
etkisi, Caco-2 hücrelerine nazaran daha fazla olmuş ve aradaki farkın
istatistiki açıdan da önemli olduğu bulunmuştur (p<0.05).

Keywords

Carpobrotus acinaciformis, antioksidan aktivite, sitotoksik etki, apoptotik etki

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