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Healing effects of L-carnitine on experimental colon anastomosis wound

Year 2023, , 215 - 222, 31.05.2023
https://doi.org/10.5472/marumj.1307971

Abstract

Objective: The purpose of this study is to examine the effects of L-carnitine on healing of experimental colon anastomosis injury in
early and late period.
Materials and Methods: Forty female Wistar-Albino rats were used in this study. The rats were divided into 4 groups (CONT-3, CONT-
7, CARN-3, and CARN-7). Injury healing was evaluated for CONT-3 group on the 3rd day and for CONT-7 group on the 7th day
following the anastomosis. Following the operation, CARN-3 and CARN-7 groups were intraperitoneally administered with 100 mg/
kg/day L-carnitine and injury healing was evaluated on the 3rd and 7th days. Injury strength, histological evaluation and antioxidant
enzyme activities and oxidant damage were determined in tissue samples of anastomosis area.
Results: Bursting pressure levels and histological scoring values of CARN-3 group were found to be higher than the CONT-3 group
(p<0.05). Antioxidant enzyme activities were found to be high in groups which were administered with L-carnitine, and oxidant
damage was found to be significantly low in CARN-7 group (p<0.05).
Conclusion: It was seen that L-carnitine speeds up the injury healing process and increases the injury strength and antioxidant capacity
in early period. Increase in antioxidant enzyme activities was observed to be continued in late period as well.

References

  • Kirchhoff P, Clavien PA, Hahnloser D. Complications in colorectal surgery: risk factors and preventive strategies. Patient Saf Surg 2010; 4:5. doi: 10.1186/1754-9493-4-5
  • Ruiz-Tovar J, Morales-Castiñeiras V, Lobo-Martínez E. Postoperative complicactions of colon surgery. Cir Cir 2010; 78:283-91.
  • Gülçin I. Antioxidant and antiradical activities of L-carnitine. Life Sci 2006; 78:803-11. doi: 10.1016/j.lfs.2005.05.103
  • Kutluay Köklü AH, Küpeli Akkol E, Uğar Çankal DA. Biochemical and biomechanical assessment of effects of L-carnitine on oral mucosal wounds. Clin Oral Investig 2015; 19:1101-6. doi: 10.1007/s00784.014.1329-8
  • Bell FP, Vidmar TJ, Raymond TL. L-carnitine administration and withdrawal affect plasma and hepatic carnitine concentrations, plasma lipid and lipoprotein composition, and in vitro hepatic lipogenesis from labeled mevalonate in normal rabbits. J Nutr 1992; 122:959-66. doi: 10.1093/ jn/122.4.959
  • Mayes PA. Lipids of physiologic significance. In: Murray RK. Granner DK. Mayes PA. Rodwell VW, eds. Harper’s Biochemistry. Appleton and Lange: Stamford, 2000: 160,171.
  • Arduini A. Carnitine and its acyl esters as secondary antioksidants? Am Heart J 1992; 123:1726-7. doi: 10.1016/0002-8703(92)90850-u
  • Sabry MM, Ahmed MM, Maksoud OMA, et al. Carnitine, apelin and resveratrol regulate mitochondrial quality control (QC) related proteins and ameliorate acute kidney injury: role of hydrogen peroxide. Arch Physiol Biochem 2020; 13:1-10. doi: 10.1080/13813.455.2020.1773504
  • Zhao T, Chen S, Wang B, Cai D. L-Carnitine Reduces Myocardial Oxidative Stress and Alleviates Myocardial Ischemia-Reperfusion Injury by Activating Nuclear Transcription-Related Factor 2 (Nrf2)/Heme Oxygenase-1 (HO-1) Signaling Pathway. Med Sci Monit 2020; 26:e923251. doi: 10.12659/MSM.923251
  • Akkus A, Aydinuraz K, Daphan C, et al. Effect of carnitine on cutaneous wound healing in immunosuppressed rats. J Surg Res 2009; 155:301-5. doi: 10.1016/j.jss.2008.06.010
  • Choi BS, Song HS, Kim HR, et al. Effect of coenzyme Q10 on cutaneous healing in skin-incised mice. Arch Pharm Res 2009; 32:907-13. doi: 10.1007/s12272.009.1613-3
  • Cakmak GK, Irkorucu O, Ucan BH, et al. The effects of resveratrol on the healing of left colonic anastomosis. J Invest Surg 2009(a); 22:353-61. doi: 10.1080/089.419.30903214701
  • Kabali B, Girgin S, Gedik E, Ozturk H, Kale E, Buyukbayram H. N-Acetylcysteine prevents deleterious effects of ıschemia/ reperfusion ınjury on healing of colonic anastomosis in rats. Eur Surg Res 2009; 43:8–12. doi: 10.1159/000210673
  • Zeytin K, Çiloğlu NS, Ateş F, Aker VF, Ercan F. Resveratrolün diyabetik sıçanlarda tendon iyileşmesi üzerine etkileri. Acta Orthop Traumatol Turc 2014;48(3):355-62. doi: 10.3944/ AOTT.2014.13.0096
  • Cakmak GK, Irkorucu O, Ucan BH, et al. Simvastatin improves wound strength after intestinal anastomosis in the rat. J Gastrointest Surg 2009(b); 13:1707-16. doi: 10.1007/ s11605.009.0951-2
  • Kurahashi T, Fujii J. Roles of Antioxidative Enzymes in Wound Healing. J Dev Biol 2015; 3:57-70. https://doi.org/10.3390/ jdb3020057
  • Xu Z, Han S, Gu Z, Wu J. Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing. Adv Healthc Mater 2020; 9:e1901502. doi: 10.1002/adhm.201901502
  • Charan J, Kantharia ND. How to calculate sample size in animal studies? J Pharmacol Pharmacother 2013; 4:303-6. doi:10.4103/0976-500X.119726
  • Arifin WN, Zahiruddin WM. Sample Size Calculation in Animal Studies Using Resource Equation Approach. Malays J Med Sci 2017; 24:101-5. doi: 10.21315/mjms2017.24.5.11
  • Demir U, Edremitlioğlu M, Kandaş E, Şehitoğlu MH, Kılınç N. Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury. Acta Cir Bras 2020; 35:e202000602. doi: 10.1590/ s0102.865.0202000.600.00002
  • Goel SA, Nagpal P, Nagarajan P, Panda A K, Chhabra HS. Lateral approach to the lumbar spine of sprague dawley rat: development of a novel animal model for spine surgery. Indian Spine J 2019; 2:134-37.
  • Irvin TT, Hunt TK. Pathogenesis and prevention of disruption of colonic anastomoses in traumatized rats. Br J Surg 1974; 61:437-39. doi: 10.1002/bjs.180.061.0605.
  • Yagi K. Assay of lipid peroxidation in blood plasma or serum. Methods Enzymol 1984; 105: 328-31. doi: 10.1016/s0076- 6879(84)05042-4
  • Chiu CJ, McArdle AH, Brown R, Scott HJ, Gurd FN. Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg 1970; 101:478-83. doi: 10.1001/archsurg.1970.013.40280030009
  • Poon P, Law WL, Chu KW, Wong J. Emergency resection and primary anastomosis for left-sided obstructing colorectal carcinoma in the elderly. Br J Surg 1998; 85:1539-42. doi: 10.1046/j.1365-2168.1998.00903.x
  • Thornton FJ, Barbul A. Healing in the gastrointestinal tract. Surg Clin North Am 1997; 77: 549-73. doi: 10.1016/s0039- 6109(05)70568-5
  • Oines MN, Krarup PM, Jorgensen LN, Agren MS. Pharmacological interventions for improved colonic anastomotic healing: a meta-analysis. World J Gastroenterol 2014;20:12637-48. doi: 10.3748/wjg.v20.i35.12637.
  • Hensley K, Kotake Y, Sang H, et al. Dietary choline restriction causes complex I dysfunction and increased H(2)O(2) generation in liver mitochondria. Carcinogenesis 2000; 21:983-9. doi: 10.1093/carcin/21.5.983
  • Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 2003; 91:179-94. doi: 10.1093/aob/mcf118
  • Bayir H. Reactive oxygen species. Crit Care Med 2005; 33(12 Suppl):S498-501. doi: 10.1097/01.ccm.000.018.6787.64500.12
  • Yager DR, Kulina RA, Gilman LA. Wound fluids: a window into the wound environment? Int J Low Extrem Wounds 2007; 6:262-72. doi: 10.1177/153.473.4607307035
  • Moradi M, Moradi A, Alemi M, et al. Safety and efficacy of clomiphene citrate and L-carnitine in idiopathic male infertility: a comparative study. Urology J 2010; 7:188-93. doi:10.22037/UJ.V7I3.750
  • Kunnavatana SS, Quan SY, Koch RJ. Combined effect of hyberbaric oxygen and N-acetylcysteine on fibroblast proliferation. Arch Otolaryngol Head Neck Surg 2005;131:809- 14. doi: 10.1001/archotol.131.9.809
  • Broughton G II, Janis JE, Attinger CE. The basic science of wound healing, Plast Reconstr Surg 2006;117: 12-34. doi: 10.1097/01.prs.000.022.5430.42531.c2
  • Ercan U, Kiraz A, Çikman Ö, et al. The effect of systemic carnitine administration on colon anastomosis healing in an experimental sepsis model. J Invest Surg 2015; 28:334-40. doi: 10.3109/08941.939.2015.1029652
  • Fang AH, Chao W, Ecker M. Review of colonic anastomotic leakage and prevention methods. J Clin Med 2020; 9:4061. doi: 10.3390/jcm9124061
  • Fathizadeh H, Milajerdi A, Reiner Ž, et al. The effects of L-carnitine supplementation on indicators of inflammation and oxidative stress: a systematic review and meta-analysis of randomized controlled trials. J Diabetes Metab Disord 2020; 19:1879-94. doi: 10.1007/s40200.020.00627-9
  • Cronin K, Jackson DS, Dunphy JE. Changing bursting strength and collagen content of the healling colon. Surg Gynecal obstent 1968; 126 : 747 – 53.
Year 2023, , 215 - 222, 31.05.2023
https://doi.org/10.5472/marumj.1307971

Abstract

References

  • Kirchhoff P, Clavien PA, Hahnloser D. Complications in colorectal surgery: risk factors and preventive strategies. Patient Saf Surg 2010; 4:5. doi: 10.1186/1754-9493-4-5
  • Ruiz-Tovar J, Morales-Castiñeiras V, Lobo-Martínez E. Postoperative complicactions of colon surgery. Cir Cir 2010; 78:283-91.
  • Gülçin I. Antioxidant and antiradical activities of L-carnitine. Life Sci 2006; 78:803-11. doi: 10.1016/j.lfs.2005.05.103
  • Kutluay Köklü AH, Küpeli Akkol E, Uğar Çankal DA. Biochemical and biomechanical assessment of effects of L-carnitine on oral mucosal wounds. Clin Oral Investig 2015; 19:1101-6. doi: 10.1007/s00784.014.1329-8
  • Bell FP, Vidmar TJ, Raymond TL. L-carnitine administration and withdrawal affect plasma and hepatic carnitine concentrations, plasma lipid and lipoprotein composition, and in vitro hepatic lipogenesis from labeled mevalonate in normal rabbits. J Nutr 1992; 122:959-66. doi: 10.1093/ jn/122.4.959
  • Mayes PA. Lipids of physiologic significance. In: Murray RK. Granner DK. Mayes PA. Rodwell VW, eds. Harper’s Biochemistry. Appleton and Lange: Stamford, 2000: 160,171.
  • Arduini A. Carnitine and its acyl esters as secondary antioksidants? Am Heart J 1992; 123:1726-7. doi: 10.1016/0002-8703(92)90850-u
  • Sabry MM, Ahmed MM, Maksoud OMA, et al. Carnitine, apelin and resveratrol regulate mitochondrial quality control (QC) related proteins and ameliorate acute kidney injury: role of hydrogen peroxide. Arch Physiol Biochem 2020; 13:1-10. doi: 10.1080/13813.455.2020.1773504
  • Zhao T, Chen S, Wang B, Cai D. L-Carnitine Reduces Myocardial Oxidative Stress and Alleviates Myocardial Ischemia-Reperfusion Injury by Activating Nuclear Transcription-Related Factor 2 (Nrf2)/Heme Oxygenase-1 (HO-1) Signaling Pathway. Med Sci Monit 2020; 26:e923251. doi: 10.12659/MSM.923251
  • Akkus A, Aydinuraz K, Daphan C, et al. Effect of carnitine on cutaneous wound healing in immunosuppressed rats. J Surg Res 2009; 155:301-5. doi: 10.1016/j.jss.2008.06.010
  • Choi BS, Song HS, Kim HR, et al. Effect of coenzyme Q10 on cutaneous healing in skin-incised mice. Arch Pharm Res 2009; 32:907-13. doi: 10.1007/s12272.009.1613-3
  • Cakmak GK, Irkorucu O, Ucan BH, et al. The effects of resveratrol on the healing of left colonic anastomosis. J Invest Surg 2009(a); 22:353-61. doi: 10.1080/089.419.30903214701
  • Kabali B, Girgin S, Gedik E, Ozturk H, Kale E, Buyukbayram H. N-Acetylcysteine prevents deleterious effects of ıschemia/ reperfusion ınjury on healing of colonic anastomosis in rats. Eur Surg Res 2009; 43:8–12. doi: 10.1159/000210673
  • Zeytin K, Çiloğlu NS, Ateş F, Aker VF, Ercan F. Resveratrolün diyabetik sıçanlarda tendon iyileşmesi üzerine etkileri. Acta Orthop Traumatol Turc 2014;48(3):355-62. doi: 10.3944/ AOTT.2014.13.0096
  • Cakmak GK, Irkorucu O, Ucan BH, et al. Simvastatin improves wound strength after intestinal anastomosis in the rat. J Gastrointest Surg 2009(b); 13:1707-16. doi: 10.1007/ s11605.009.0951-2
  • Kurahashi T, Fujii J. Roles of Antioxidative Enzymes in Wound Healing. J Dev Biol 2015; 3:57-70. https://doi.org/10.3390/ jdb3020057
  • Xu Z, Han S, Gu Z, Wu J. Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing. Adv Healthc Mater 2020; 9:e1901502. doi: 10.1002/adhm.201901502
  • Charan J, Kantharia ND. How to calculate sample size in animal studies? J Pharmacol Pharmacother 2013; 4:303-6. doi:10.4103/0976-500X.119726
  • Arifin WN, Zahiruddin WM. Sample Size Calculation in Animal Studies Using Resource Equation Approach. Malays J Med Sci 2017; 24:101-5. doi: 10.21315/mjms2017.24.5.11
  • Demir U, Edremitlioğlu M, Kandaş E, Şehitoğlu MH, Kılınç N. Quercetin associated with dimethylsulfoxide has a curative effect on experimental colon anastomosis injury. Acta Cir Bras 2020; 35:e202000602. doi: 10.1590/ s0102.865.0202000.600.00002
  • Goel SA, Nagpal P, Nagarajan P, Panda A K, Chhabra HS. Lateral approach to the lumbar spine of sprague dawley rat: development of a novel animal model for spine surgery. Indian Spine J 2019; 2:134-37.
  • Irvin TT, Hunt TK. Pathogenesis and prevention of disruption of colonic anastomoses in traumatized rats. Br J Surg 1974; 61:437-39. doi: 10.1002/bjs.180.061.0605.
  • Yagi K. Assay of lipid peroxidation in blood plasma or serum. Methods Enzymol 1984; 105: 328-31. doi: 10.1016/s0076- 6879(84)05042-4
  • Chiu CJ, McArdle AH, Brown R, Scott HJ, Gurd FN. Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg 1970; 101:478-83. doi: 10.1001/archsurg.1970.013.40280030009
  • Poon P, Law WL, Chu KW, Wong J. Emergency resection and primary anastomosis for left-sided obstructing colorectal carcinoma in the elderly. Br J Surg 1998; 85:1539-42. doi: 10.1046/j.1365-2168.1998.00903.x
  • Thornton FJ, Barbul A. Healing in the gastrointestinal tract. Surg Clin North Am 1997; 77: 549-73. doi: 10.1016/s0039- 6109(05)70568-5
  • Oines MN, Krarup PM, Jorgensen LN, Agren MS. Pharmacological interventions for improved colonic anastomotic healing: a meta-analysis. World J Gastroenterol 2014;20:12637-48. doi: 10.3748/wjg.v20.i35.12637.
  • Hensley K, Kotake Y, Sang H, et al. Dietary choline restriction causes complex I dysfunction and increased H(2)O(2) generation in liver mitochondria. Carcinogenesis 2000; 21:983-9. doi: 10.1093/carcin/21.5.983
  • Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 2003; 91:179-94. doi: 10.1093/aob/mcf118
  • Bayir H. Reactive oxygen species. Crit Care Med 2005; 33(12 Suppl):S498-501. doi: 10.1097/01.ccm.000.018.6787.64500.12
  • Yager DR, Kulina RA, Gilman LA. Wound fluids: a window into the wound environment? Int J Low Extrem Wounds 2007; 6:262-72. doi: 10.1177/153.473.4607307035
  • Moradi M, Moradi A, Alemi M, et al. Safety and efficacy of clomiphene citrate and L-carnitine in idiopathic male infertility: a comparative study. Urology J 2010; 7:188-93. doi:10.22037/UJ.V7I3.750
  • Kunnavatana SS, Quan SY, Koch RJ. Combined effect of hyberbaric oxygen and N-acetylcysteine on fibroblast proliferation. Arch Otolaryngol Head Neck Surg 2005;131:809- 14. doi: 10.1001/archotol.131.9.809
  • Broughton G II, Janis JE, Attinger CE. The basic science of wound healing, Plast Reconstr Surg 2006;117: 12-34. doi: 10.1097/01.prs.000.022.5430.42531.c2
  • Ercan U, Kiraz A, Çikman Ö, et al. The effect of systemic carnitine administration on colon anastomosis healing in an experimental sepsis model. J Invest Surg 2015; 28:334-40. doi: 10.3109/08941.939.2015.1029652
  • Fang AH, Chao W, Ecker M. Review of colonic anastomotic leakage and prevention methods. J Clin Med 2020; 9:4061. doi: 10.3390/jcm9124061
  • Fathizadeh H, Milajerdi A, Reiner Ž, et al. The effects of L-carnitine supplementation on indicators of inflammation and oxidative stress: a systematic review and meta-analysis of randomized controlled trials. J Diabetes Metab Disord 2020; 19:1879-94. doi: 10.1007/s40200.020.00627-9
  • Cronin K, Jackson DS, Dunphy JE. Changing bursting strength and collagen content of the healling colon. Surg Gynecal obstent 1968; 126 : 747 – 53.
There are 38 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Original Research
Authors

Emel Kandas This is me 0000-0001-9845-3232

Mustafa Edremıtlıoglu This is me 0000-0002-0510-9852

Ufuk Demır This is me 0000-0002-0020-1633

Guven Erbıl This is me 0000-0002-8448-0691

Muserref Hilal Sehıtoglu This is me 0000-0003-4436-9160

Publication Date May 31, 2023
Published in Issue Year 2023

Cite

APA Kandas, E., Edremıtlıoglu, M., Demır, U., Erbıl, G., et al. (2023). Healing effects of L-carnitine on experimental colon anastomosis wound. Marmara Medical Journal, 36(2), 215-222. https://doi.org/10.5472/marumj.1307971
AMA Kandas E, Edremıtlıoglu M, Demır U, Erbıl G, Sehıtoglu MH. Healing effects of L-carnitine on experimental colon anastomosis wound. Marmara Med J. May 2023;36(2):215-222. doi:10.5472/marumj.1307971
Chicago Kandas, Emel, Mustafa Edremıtlıoglu, Ufuk Demır, Guven Erbıl, and Muserref Hilal Sehıtoglu. “Healing Effects of L-Carnitine on Experimental Colon Anastomosis Wound”. Marmara Medical Journal 36, no. 2 (May 2023): 215-22. https://doi.org/10.5472/marumj.1307971.
EndNote Kandas E, Edremıtlıoglu M, Demır U, Erbıl G, Sehıtoglu MH (May 1, 2023) Healing effects of L-carnitine on experimental colon anastomosis wound. Marmara Medical Journal 36 2 215–222.
IEEE E. Kandas, M. Edremıtlıoglu, U. Demır, G. Erbıl, and M. H. Sehıtoglu, “Healing effects of L-carnitine on experimental colon anastomosis wound”, Marmara Med J, vol. 36, no. 2, pp. 215–222, 2023, doi: 10.5472/marumj.1307971.
ISNAD Kandas, Emel et al. “Healing Effects of L-Carnitine on Experimental Colon Anastomosis Wound”. Marmara Medical Journal 36/2 (May 2023), 215-222. https://doi.org/10.5472/marumj.1307971.
JAMA Kandas E, Edremıtlıoglu M, Demır U, Erbıl G, Sehıtoglu MH. Healing effects of L-carnitine on experimental colon anastomosis wound. Marmara Med J. 2023;36:215–222.
MLA Kandas, Emel et al. “Healing Effects of L-Carnitine on Experimental Colon Anastomosis Wound”. Marmara Medical Journal, vol. 36, no. 2, 2023, pp. 215-22, doi:10.5472/marumj.1307971.
Vancouver Kandas E, Edremıtlıoglu M, Demır U, Erbıl G, Sehıtoglu MH. Healing effects of L-carnitine on experimental colon anastomosis wound. Marmara Med J. 2023;36(2):215-22.