Cholinergic cognitive enhancer effect of Salvia triloba L. essential oil inhalation in rats

Year 2023, , 361 - 370, 29.09.2023

Gulsah Beyza Ertosun Mehmet Ergen , Hilal Bardakcı Timur Hakan Barak , Guldal Suyen

https://doi.org/10.5472/marumj.1368345

Cited By: 2

Abstract

Objective: Current treatment of Alzheimer’s disease is provided by cholinesterase inhibitors. Salvia triloba L. (syn. Salvia fruticosa Mill.),
a species mostly consumed as refreshing herbal tea in traditional medicine, is rich in 1,8-cineole that is known to have cholinesterase
inhibiting effects. In this study, we investigated cognitive enhancer effects of S. triloba essential oil inhalation on healthy control rats
and rats with scopolamine induced memory impairment.
Materials and Methods: S. triloba samples from different geographical locations of Turkey were hydro-distilled and analyzed by Gas
Chromatography-Mass Spectrometry (GC-MS). The optimum sample with the highest 1,8-cineole and lowest camphor, α-thujone and
β-thujone content was selected. In vitro cholinergic and antioxidant potentials of the selected essential oil were calculated. Cognitive
enhancer and anti-amnestic effects of the inhaled essential oil on rats were assessed by means of Morris water maze. The bioavailability
of 1,8-cineole in blood of rats was measured by GC-MS.
Results: The group that inhaled S. triloba significantly outperformed control group, namely faster achieving peak escape latency
performance in Morris water maze. However, S. triloba inhalation failed to restore scopolamine induced learning impairment.
Conclusion: In this study, we report positive effects of inhaling S. triloba essential oil as a complementary treatment for supporting
cognitive functions.

Keywords

Cognitive enhancers, Essential oil, Gas chromatography, 1, 8-Cineole, Antioxidants, Morris water maze

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