The role of epidermal growth factor and cholinergic receptor agonists and antagonists in MAPK signal transduction in K562 cells

Year 2024, , 86 - 91, 28.01.2024

Selda Güler Atmaca , Banu Aydın , Hulya Cabadak

https://doi.org/10.5472/marumj.1379868

Abstract

Objective: Muscarinic receptors (M1-M5) are members of the G protein-coupled receptor superfamily and are effective in physiological
functions through G proteins. Recent studies suggested that cholinergic receptors mediate cellular activities in hematopoietic cells.
The aim of this study was to investigate the potential role of mitogen-activated protein kinases (MAPK) signaling extracellular signalregulated
kinases 1 and 2 (ERK1/2)/phosphorylated ERK1/ (pERK1/2) pathways in chronic myeloid leukemia (K562) cells.
Materials and Methods: Chronic myeloid leukemia cells were cultured. Cells were incubated in the presence of muscarinic receptor
agonist, antagonist and epidermal growth factor (EGF). To detect MAPK activation, ERK/pERK protein expression levels were
determined by western blot method techniques.
Results: Our study results showed that cholinergic agents and EGFs affect the MAPK pathway in the human K562 cell line.
Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.
Conclusion: Cholinergic and EGF receptors may affect the MAPK pathway in K562 cells.

Keywords

G protein coupled receptor, Carbachol, Epidermal growth factor, Mitogen-activated protein kinase, Chronic myeloid leukemia

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