Phosphorylation of elf-2(3 and ef-1 by two ribosoflial kinase fractions
Abstract
Two ribosomal kinase fractions have been partially purified from reticulocyte lysates by DEAE-cellulose and phosphocellulose column chromatography. These fractions autophosphorylated the majority of the bands and phosphorylated EF-1 and elF-2|3. Addition of GSSG (oxidized glutathione) produced a general decrease in phosphorylation. However, phosphorylation of elF-2|3 was enhanced in the presence of GSSG.
Keywords
References
- Tuazon PT. Merrick WC. Traugh JA. Comparative analysis of phosphorylation of translational Initiation and elongation factors by seven protein kinases. JBIolChem 1989:264.2773-2777.
Details
Primary Language
English
Subjects
Clinical Sciences
Journal Section
Research Article
Publication Date
December 1, 1993
Submission Date
October 24, 2016
Acceptance Date
October 1, 1993
Published in Issue
Year 1993 Volume: 6 Number: 4