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Abstract

Objectives: The aim of this study is to investigate the effects of different concentrations of albumin, which has antioxidant characteristics on renal antioxidant levels and renal functions in rats with sepsis.Materials and Methods: Twenty-eight adult male Sprague-Dawley rats were divided into 4 groups. No agent was implemented to Group K. Group S received 30 ml/kg of saline infusion for 90 min, Group A5 received 15 ml/kg of 5% albumin infusion for 90 min and Group A25 received 3 ml/kg of 25% albumin infusion for 45 min. At the end of the 18th hour, bilateral nephrectomy was performed. Blood samples were taken to determine blood urea nitrogen, creatinine and albumin levels. Kidney tissue samples were dissected to measure the levels of free oxygen radicals and antioxidants.Results: Better renal function, higher levels of glutathione and malondialdehyde with lower myeloperoxidase levels were obtained in septic rats following treatment with 2 different concentrations of albumin. In Group A25, blood urea nitrogen and renal glutathione levels were significantly higher than in Group A5. Conclusion: We are of the opinion that the rapid infusion of 25% albumin may prevent or delay the occurrence of renal dysfunction in patients with sepsis.

Keywords

• Sepsis, Albumin, Oxygen radicals, Antioxidants

Sepsis oluşturulan sıçanlarda farklı konsantrasyonlarda verilen albuminin böbrek antioksidan ve serbest oksijen radikalleri üzerine etkisi

Abstract

Amaç: Bu çalışmanın amacı sepsis oluşturulan sıçanlarda antioksidan özellikler gösteren albuminin farklı konsantrasyonlarının böbrek antioksidan düzeyleri ve böbrek fonksiyonları üzerine etkilerini araştırmaktır. Gereç ve Yöntem: Çalışmada 28 adet erişkin Sprague-Dawley sıçan 4 gruba ayrıldı. Birinci gruba (Grup K) herhangi bir ajan uygulanmadı. Diğer gruplarda intraabdominal sepsis oluşturuldu. İkinci gruba (Grup S) 90 dk süreyle 30 ml/kg serum fizyolojik infüzyonu, 3. gruba (Grup A5) 90 dk süreyle 15 ml/kg %5 albumin infüzyonu, 4. gruba (Grup A25) ise 45 dk süreyle 3 ml/kg %25 albumin infüzyonu uygulandı. 18. saatin sonunda tüm sıçanlara anestezi altında bilateral nefrektomi yapıldı. Kan üre nitrojeni, kreatinin ve albumin düzeylerini saptamak için böbrek venlerinden kan örnekleri alındı. Böbrek dokuları elektron mikroskobu altında diseke edilerek serbest oksijen radikalleri ve antioksidan düzeylerinin ölçümü yapıldı. Bulgular: Çalışmamızda serum fizyolojik uygulanan grupla karşılaştırıldığında her 2 konsantrasyonda albumin uygulanan septik sıçanlarda böbrek fonksiyonlarının daha iyi, glutatyon düzeylerinin daha yüksek, malondialdehit ve myeloperoksidaz düzeylerinin daha düşük olduğu saptandı. Ek olarak A25 grubunda kan üre nitrojeni ve böbrek glutatyon düzeyleri A5 grubuna göre belirgin yüksekti. Sonuç: Böbrek dokusunda antioksidan etkilerini saptadığımız %25 albuminin hızlı infüzyonunun sepsis olgularında kullanımının böbrek disfonksiyonu gelişmesini önleme veya geciktirme açısından yüz güldürücü olabileceği düşüncesine varılmıştır.

Keywords

• Sepsis, Albumin, Oksijen radikalleri, Antioksidan ajanlar

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