SEREBRAL İSKEMİ/REPERFÜZYONA BAĞLI OKSİDAN BEYİN HASARINA KARŞI MELATONİN VE AMLODİPİNİN KORUYUCU ETKİLERİ

Abstract

Amaç: Bu çalışmada melatonin ve amlodipinin iskemi/reperfüzyon (İ/R) ile oluşan beyin hasarına karşı olası koruyucu etkileri araştırıldı. Yöntem: Wistar Albino sıçanlar 15 dakika bilateral carotid arter oklüzyonunu takiben 24 saat süreyle reperfüzyona bırakıldı. Melatonin ve amlodipin 10mg/kg ip veya 50µg/sıçan icv dozlarında reperfüzyondan hemen önce uygulandı. Nörolojik tayin sonrası hayvanlar dekapite edildi. Beyin dokularında malondialdehit (MDA) ve glutatyon (GSH) düzeyleri ile myeloperoxidaz (MPO) ve Na+-K+-ATPaz aktiviteleri tayin edildi. Dokuda serbest radikal oluşumu luminol ve lusigenin kemiluminesans (KL) yöntemi ile ölçüldü. Beyin ödemi yaş/kuru ağırlık üzerinden, kan beyin bariyer (KBB) geçirgenliği de Evans Mavisi (EM) extravazasyonu ile değerlendirilidi. Bulgular: Çözücü uygulanan grup ile karşılaştırıldığında nörolojik bozuklukların melatonin ve amlodipin gruplarında düzeldiği belirlendi. İskemi/reperfüzyon beyinde GSH ve Na+-K+-ATPaz aktivitesinde azalma ve bununla birlikte MDA, MPO ve KL düzeylerinde artışa neden oldu. Buna karşılık melatonin ve amlodipin tedavileri tüm incelenen biyokimyasal parametrelerdeki değişimi ve İ/R nin neden olduğu beyin ödemini geri çevirdi. Sonuç: Bu bulgulara göre gerek melatonin ve gerekse amlodipin muhtemelen antioksidan özellikleri aracılığı ile global iskemiye bağlı olarak meydana gelen nörokimyasal ve davranışsal değişikleri module edebileceği düşünülmektedir.

Anahtar Kelimeler: melatonin, amlodipin, beyin, iskemi / reperfüzyon, lipid peroksidasyonu

Keywords

• melatonin, amlodipin, beyin, iskemi / reperfüzyon, lipid peroksidasyonu

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