HAYATIN ERKEN DÖNEMİNDE KARŞILAŞILAN SOSYAL İZOLASYON STRESİ, KOLON İNFLAMASYONUNUN ŞİDDETİNİ AZALTIR

Abstract

Amaç: Hayatın erken dönemindeki stresin, ileri yaştaki akut barsak inflamasyonu ve strese maruz kalma üzerine etkilerini araştırmak. Material Metod: Dişi Sprague–Dawley sıçanlara, doğum sonrası 21. günde, sosyal izolasyon stresi uygulandı. 250 g. ağırlığa ulaşılana dek beklendi. 60 günlük protokolün son 3 gününde, izole edilen, edilmeyen gruplara sudan kaçınma stresi (WAS) 30 dak./gün uygulandı. Sıçanların kalan yarısında ise izole olan, olmayan gruplara, kolona %5'lik asetik asit verilerek kolit oluşturuldu. Son WAS uygulamasından hemen sonra ya da kolit indüksiyonunun 4. gününde sıçanlar dekapite edilerek, TNF-alpha seviyelerinin ölçümü için kan, histolojik değerlendirme, doku myeloperoksidaz (MPO) aktivitesi, nötrofil infiltrasyonunun göstergesi, ölçümü ve glutatyon (GSH) seviyelerinin, antioksidan, ölçümü için kolon dokusu alındı. Bulgular: İzole olan, olmayan guruplarda, WAS ve kolit indüksiyonu serum TNF-alpha, doku MDA seviyelerini, MPO aktivitelerini kontrole göre anlamlı arttırmıştır (p

Keywords

• myeloperoksidaz, kolit, psikolojik stres, sudan kaçınma stresi, sosyal izolasyon

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