SULU SARIMSAK ÖZÜTÜNÜN SUDAN KAÇINMA STRESİNİN TETİKLEDİĞİ DERİDEKİ MAST HÜCRE DEGRANÜLASYONUNA KARŞI KORUYUCU ETKİSİ

Abstract

Amaç: Bu çalışmanın amacı sulu sarımsak özütü (=aqueaous garlic extract, AGE)’ nün, sudan kaçınma stresi (SKS)’ nin tetiklediği derideki mast hücre degranülasyonu üzerindeki etkilerinin araştırılmasıdır.

Gereç ve Yöntem: Wistar albino sıçanlar, kronik sudan kaçınma stresi (SKS grubu)’ ne maruz bırakıldı. Hayvanlar SKS’ne maruz bırakıldıktan sonra 250mg/kg AGE i.p. olarak enjekte edildi (SKS + AGE grubu). Dermal mast hücreleri toluidin mavisi ile boyanarak ışık mikroskopi düzeyinde incelendi.

Sonuçlar: SKS grubunda hem granüllü hem de degranüle olmuş mast hücrelerinin sayısı kontrol grubundakilere oranla artmıştır. SKS + AGE grubundaki granüllü ve degranüle olmuş olgun mast hücrelerinin sayısı SKS grubundakilere oranla azalmıştır.

Tartışma: Kronik AGE tedavisi SKS’nin tetiklediği dermisteki mast hücre artışını ve yoğunluğunu azaltmıştır ve stresin tetiklediği deri rahatsızlıklarında tedavi edici ajan olarak kullanımı çalışmalara açık görülmektedir.

Anahtar Kelimeler: Deri, Mast hücresi, Sudan kaçınma stresi, Sulu sarımsak özütü

Keywords

• Deri, Mast hücresi, Sudan kaçınma stresi, Sulu sarımsak özütü

References

1. 1. Chrousos GP, Gold PW. The concepts of stress system disorders: overview of behavioral and physical homeostasis. JAMA 1992; 267:1244-1252.
2. 2. Theoharides TC, Cochrane DE. Critical role of mast cells in inflammatory diseases and the effect of acute stress. J Neuroimmunology 2004; 146:1–12.
3. 3. Sugiura H, Uehara M. Mitosis of mast cells in skin lesions of atopic dermatitis. Acta Derm Venereol 1993; 73:296–299.
4. 4. Huston DP, Bressler RB. Urticaria and angioedema. Med Clin North Am 1992; 76:805–840.
5. 5. Ozdamar SO, Seckin D, Kandemir B, Turanli AY. Mast cells in psoriasis. Dermatology 1996; 192:189–190.
6. 6. Farber EM, Nickoloff BJ, Recht B, Fraki JE. Stress, symmetry, and psoriasis: Possible role of neuropeptides. J Am Acad Dermatol 1986; 14:305–311.
7. 7. Mihm MC, Soter NA, Dvorak HF, Austen KF. The structure of normal skin and the morphology of atopic eczema. J Invest Dermatol 1976; 67:305–311. 8. Theoharides TC, Singh LK, Boucher W, et al.
8. Corticotropin-releasing hormone induces skin mast cell degranulation and increased vascular permeability, a possible explanation for its proinflammatory effects. Endocrinology 1998; 139:403-413.

9. 9. Christian EP, Undem BJ, Weinreich D. Endogenous histamine excites neurons in the guinea-pig superior cervical ganglion in vitro. J Physiol 1989; 409:297–312.
10. 10. Goetzl EJ, Chernov T, Renold F, Payan DG. Neuropeptide regulation of the expression of immediate hypersensitivity. J Immunol 1985; 135:802–805.
11. 11. Cochrane DE, Emigh C, Levine G, Carraway RE, Leeman SE. Neurotensin alters cutaneous vascular permeability and stimulates histamine release from isolated skin. Peptides 1982; 12:1187–1194.
12. 12. Matsuda H, Kawakita K, Kiso Y, Nakano T, Kitamura Y. Substance P induces granulocyte infiltration through degranulation of mast cells. J Immunol 1989; 142:927–931.
13. 13. Slominski A, Wortsman J, Luger T, Paus R, Solomon S. Corticotropin releasing hormone and proopiomelanocortin involvement in the cutaneous response to stress. Physiol Rev 2000; 80:979–1020.
14. 14. Dorant E, van den Brandt PA, Goldbohm R, Hermus RJ, Sturmans F. Garlic and its significance for the prevention of cancer in humans: a critical view. Br J Cancer 1993; 67: 424–429.
15. 15. Lamm DL, Riggs DR. Enhanced immunocompetence by garlic: role in bladder cancer and other malignancies. J Nutr 2001; 131(3s):1067S-1070S.
16. 16. Chung JG. Effects of garlic components diallyl sulfide and diallyl disulfide on arylamine Nacetyltransferase activity in human bladder tumor cells. Drug Chem Toxicol 1999; 22(2):343-358.
17. 17. Kleijnen J, Knipschild P, ter Riet G. Garlic, onions and cardiovascular risk factors. A rewiew of the evidence from human experiments with emphasis on commercially available preparations. Br J Clin Pharmacol 1989; 28:535–544.
18. 18. Nakagawa S, Kasuga S, Matsuura H. Prevention of liver damage by aged garlic extract. Phytother Res 1989; 3:50–53.
19. 19. Moriguchi T, Takashina K, Chu P, Saito H, Nishiyama N. Prolongation of life span and improved learning in the senescence accelerated mouse produced by aged garlic extract. Bioll Pharm Bull 1994; 17:1589–1594.
20. 20. Rietz B, Isensee H, Strobach H, Makdessi S, Jacob R. Cardioprotective actions of wild garlic (Allium ursinum) in ischemia and reperfusion. Mol Cell Biochem 1993; 119:143–150.
21. 21. Numagami Y, Suto S, Ohnishi ST. Attenuation of rat ischemic brain damage by aged garlic extracts: a possible protecting mechanism as antioxidants. Neurochem Int 1996; 29: 135–143.
22. 22. Rabinkov A, Miron T, Konstantinovski L, Wilchek M, Mirelman D, Weiner L. The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochem Biophys Acta 1998; 1379:233–244.
23. 23. Augusti KT, Sheela CG. Antiperoxide effect of Sallyl cysteine sulfoxide, an insulin secretagogue, in diabetic rats. Experimentia 1996; 52:115–120.
24. 24. Santos J, Benjamin M, Yang PC, Prior T, Perdue MH. Chronic stress impairs rat growth and jejunal epithelial barrier function: role of mast cells. Am J Physiol Gastrointest Liver Physiol 2000; 278:847- 854.
25. 25. Yokoyama K, Uda N, Takasugi N, Fuwa T. Antistress effects of garlic extract preparation containing vitamins (Kyoleopin) and ginseng-garlic preparation containing vitamin B1 (Leopin-five) in mice. Oyo Yakuri (Applied Pharmacology) 1986; 31: 977-984
26. 26. Kyo E, Uda N, Ushijima M, Kasuga S, Itakura Y. Prevention of psychological stress-induced immune suppression by aged garlic extract. Phytomedicine 1999; 6:325-330.
27. 27. Kyo E, Uda N, Kasuga S, Itakura Y. Supplement: Recent advances on the nutritional effects associated with the use of garlic as a supplement.