Iran Tebriz Ostad Alinasab Hastanesi’nin örneklerinden elde edilen Escherichia coli izolatlarında genişletilmiş-spektrum beta-laktamoz (TEM1) geninin araştırılması

Year 2016, Volume: 29 Issue: 1, 35 - 40, 04.03.2016

Azizeh Dizaji Azizeh Shadi Dizajı Reza Fathi Abolfazl Sales Abolfazl Jafari Sales

https://doi.org/10.5472/MMJoa.2901.06

Cited By: 6

Abstract

Amaç: Antibiyotik direncinin gelişmesi bakteriyel enfeksiyonların
kontrolünde önemli bir sorundur. Bu çalışma, antibiyotik
duyarlılık profillerini değerlendirmek ve Tebriz’de Ostad Alinasab
Hastane’sinin klinik örnekleriden elde edilen E.coli izolatlarında
blaTEM geni varlığını değerlendirmek amacıyla planlanmıştır.
Gereç ve Yöntemler: E. coli (100 izolatları) geleneksel bakteriyolojik
testler ile elde edilmiş ve daha sonra antibiyotic duyarlılık
testleri Kirby-Bauer yöntemine göre değerlendirilmiştir.
Doğrulayıcı testler ise “Combinde disk” testleri ile gerçekleştirilmiştir.
Son olarak blaTEM geni varlığı polymeraz zincir reaksiyon
(PCR) tekniği kullanılarak değerlendirilmiştir.
Bulgular: Yüz E. coli izolatından %23’nün blaTEM genini
içerdiği belirlenmiştir. İzolatlarının %18’inde Seftazidim, %
30’unda sefotaksim direnci saptanmıştır. Izolatların % 46’sında
EBSL sentezi belirlenmiş ve bu izolatların % 23’de de blaTEM
geninin varlığı saptanmıştır.
Sonuç: E. coli izolatlarında saptanan üçüncü kuşak sefalosporinlere
karşı yüksek direnç uygunsuz antibiyotik kullanımından
kaçınılması gerektiğinin ve tedavi öncesi doğru duyarlılık testlerinin
uygulanması gerektiğinin önemin göstermektedir.

Keywords

Geniş spektrum beta-laktamaz (GSBL), blaTEM geni, Escherichia coli, Polimeraz zincir reaksiyonu

References

• 1. Joklik WK, Willett HS, Wilfert C H. (eds) Antimicrobial agents. Zinsser Microbiology. 20th ed. Norwalk: Appleton and Lange, 2005: 153-87.
• 2. Al-Jasser A M. Extended-spectrum beta-lactamases (ESBLs): A global problem.Kuwait Med J 2006;38;171-85.
• 3. Medeiros A A. Evolution and dissemination of ß-lactamases accelerated by generations of ß-lactam antibiotics. Clin Infect Dis 1997;997:(Suppl 1):S19-45.
• 4. Koseoglu O, Kocaguz S, Gur D, Akova M. Nosocomial blood stram infection in Turkish university hospital : Study of Gramnegative bacilli and their sensitivity patterns, Int J Antimicrob Agents 2001;17:477-81.
• 5. Rupp M E, Fey P D. Extended - spectrum beta-lactamse (ESBL) producing Enterobacteriaceae: Consideration for diagnosis, prevention and drug treatment. Drugs 2003;63:353- 65.
• 6. Thomson K D, Preevan AM, Sanders CC. Novel plasmedmediated beta- lactamases in Enterobacteriaceae: emerging problems for new beta-lactam antibiotic. Curr Clin Top Infect Dis 1996;16:151-63.
• 7. Ensor VM, Livermore DM, Hawkey PM. A novel reverseline hybridization assay for identifying genotypes of CTX-MTypeextended-spectrum beta-lactamases. Antimicrob Chemother 2007;59:387-95.
• 8. Biedenbach DJ, Lewis MT, Gones RN. Invitro evaluation of cefepime and other road-spectrum p-Iactams for isolates in Malaysia and Singapore Antimicrobial Resistance Study group. Diagn Microbiol Infect Dis 1999;35:277- 83.
• 9. Stürenburg E, Mack D. Extended-spectrum beta-lactamases: implications for the clinical microbiology laboratory, therapy, and infection control. J Infect 2003;47:273-95.
• 10. Lal P, Kapil A, Das B K, Sood S. Occurrence of TEM and SHV gene in extended-spectrum beta- lactamases ( ESBLS ) producing Klebsiella sp. Isolated from a tertiary care hospital. Indian Med Res 2007;125:173-8.
• 11. Colom K, Perez J, Alonso R, Aranguiz AF, Larino E, Cisterna R. Simple and reiable multiplex PCR assay for detection of blaTEM genes in Enterobacteriaceae. FEMS Microbiol Lett 2003;223:147-51.
• 12. Zamberi S, Rusmah Y, Rusmah M. Extended- spectrum beta- lactamases- producing Escherichia coli from a tertiary hospital in Malaysia: emergence of CTX- M- type betalactamases variation. Res Microbiol 2008;125: 1-5.
• 13. Hosseini-Mazinani SM, Eftekhar F, Milani M, Ghandili S. Characterization of beta-lactamases from urinary isolates of Escherichia coli in Tehran. Iran Biomed J 2007;11:95-9.
• 14. Masjedian GF, Valehi F, Talebi A, Rastegar LA. Moulecular evaluation of resistance to espanded antibiotics in Escherichia coli and Klebsiella pneumoniae. Iran J Med Microbiol 2007;1:27-34.
• 15. Mirsalehian A, Akbari-Nakhjavani F, Peymani A, Kazemi B, JabalAmeli F, Mirafshar SM. Prevalence of extended spectrum β-lactamase-producing Enterobacteriaceae by phenotypic andgenotypic methods in intensive care units in Tehran, Iran. Daru 2008;16:169-73.
• 16. Hernández MS, %ADa%20 JA%225BAuthor%D&itool=EntrezSystem.PEntrez. Pubmed.Pubmed_ResultsPanel.Pubmed_Discovery Panel.Pubmed_RVAbstractPlus”GarcíaHYPERLINK “http://www.ncbi.nlm.nih.gov/sitesentrez?Db=pubmed &Cmd=Search&Term=%22Garc%C3%ADa%20 JA%22%5BAuthor%5D&itool=EntrezSystem2. PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_ DiscoveryPanel.Pubmed_RVAbstractPlus”JA, %Boz%20 JL%225BAuthor%D&itool=EntrezSystem.PEntrez.Pubmed. Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_ RVAbstractPlus”Muñoz JL In vitro activity of fosfomycin against ESBL-producing enterobacteria of urinary origin. Rev Esp Quimioter. 2009;22:25-9.
• 17. Bradford PA. Extended-spectrum beta-lactamases in the 21stcentury: characterization, epidemiology, and detection of thisimportant resistance threat. Clin Microbiol Rev 2001;14:933-51. doi: 10.1128/CMR.14.4.933-951.2001
• 18. Fang H, Ataker F, Hedin G, Dornbusch K. Molecular epidemiology of extended-spectrum beta-lactamases among Escherichia coli isolates collected in a Swedish hospital and its associated health care facilities from 2001 to 2006. J Clin Microbiol 2008;46:707-12. doi: 10.1128/JCM.01943-07
• 19. Pai H, Lee HJ, Choi E H, Kim J, Jacoby GA. Evolution of TEM-related extended-spectrum beta-lactamases in Korea. Anti Microb Agent Chemother 2001;45: 3651-3.
• 20. Pornour MR, Nahaie Mobaien H, Mobasher Karjedi A. Molecular study of Eutended- Spectrum Beta- Lactamaase (TEM type) in Escherichia coli and Klebsiella pneumoniae isolates from clinical samples, Med J Tabriz Univ Med 2010;32:30-4.
• 21. Soltan Dallal M M, Molla Aghamirzaei H, Fallah Mehrebadi J, Rastegar Lari A, Sabbaghi A, Eshraghian MR. Molecular detection of TEM and Ampc (OHA, Mox) broad spectrum B- lactamase in clinical isolates. Tehran Univ Med J 2010;68:315-20.
• 22. Zamanzad B, Deiham B, molecular detectin of TEM1 type beta- lactamasegenes in Escherichia coli, Klebsiella pnonomoniae, Entr, bacter from clinical Isolated Educational hospitals in Shahrkord. J Hamadan Med Univ 2007;14:27-34.
• 23. Shojapour M, Shariati L, Karimi A, Zamanzad B. Prevalence of TEM-1 type beta-lactamase genes in Pseudomonas aeruginosa strains isolated from burn infections using Duplex PCR in Shahrekord. 2011:14:55-61.
• 24. Lin CF, Hsu SK, Chen CH, Huang JR, Lo HH. Genotypic detection and molecular epidemiology of extended- spectrum beta lactamase producing Escherichia coli and Klebsiella pneamoniae in a regional hospital in central Taiwan. J Med Microbiol 2010; 56:665- 71. doi: 10.1099/jmm.0.015818-0.
• 25. Shakil, SH., Akram, M,. et al, 2010. Acguisition of entendedspectrum- lactomase produsing Escherichia coli strains in male and female infants admitted to a neonatal intensive care unit: molecular epidmiogy and analysis of risk factors, Journal Med microbial. 59: 948- 954.