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Year 1994, Volume: 7 Issue: 3, 115 - 118, 01.08.1994

Abstract

References

  • Schinner Rfi, Krauth-Siegel RL, Schulz OE. Olutat- hione reductase. In: Dolphin D. Poulson R, Avramovic O, eds. Qlutathione: Chemical, biochemical and medical aspects. Part A. /Yew York: John Wiley and Sons, 1989:553-596.

İnhibition of brain glutathione reductase by sodium arsenite

Year 1994, Volume: 7 Issue: 3, 115 - 118, 01.08.1994

Abstract

The aim of this study is to investigate the inhibition characteristics of brain glutathione reductase (GSSGR) under reducing conditions. Sheep brain glutathione reductase (GSSGR, NAD(P)H: oxidized glutathione oxidoreductase, EC 1.6.4.2) was purified about 1 1000 fold with a method employing ammonium sulphate fractionation, heat denaturation, 2',5‘-ADP Sepharose 4B and Sepharose C16B chromatography steps. NADPH is an inhibitor of brain GSSGR. Sodium arsenite, which is a specific inhibitor of enzymes having two nascent -SH groups, had little inhibitory effect on GSSGR when incubated with the enzyme alone. When the enzyme was incubated with both NADPH and arsenite, inhibition was intensified.

2-Mercaptoethanol and reduced glutathione enhanced the inhibition further; whereas oxidized glutathione protected the enzyme from inhibition. The reaction mechanism of the enzyme is discussed in the light of these findings.

References

  • Schinner Rfi, Krauth-Siegel RL, Schulz OE. Olutat- hione reductase. In: Dolphin D. Poulson R, Avramovic O, eds. Qlutathione: Chemical, biochemical and medical aspects. Part A. /Yew York: John Wiley and Sons, 1989:553-596.
There are 1 citations in total.

Details

Subjects Clinical Sciences
Journal Section Review Makaleler
Authors

N.l. Açan This is me

E.f. Tezcan This is me

Publication Date August 1, 1994
Published in Issue Year 1994 Volume: 7 Issue: 3

Cite

APA Açan, N., & Tezcan, E. (1994). İnhibition of brain glutathione reductase by sodium arsenite. Marmara Medical Journal, 7(3), 115-118.
AMA Açan N, Tezcan E. İnhibition of brain glutathione reductase by sodium arsenite. Marmara Med J. August 1994;7(3):115-118.
Chicago Açan, N.l., and E.f. Tezcan. “İnhibition of Brain Glutathione Reductase by Sodium Arsenite”. Marmara Medical Journal 7, no. 3 (August 1994): 115-18.
EndNote Açan N, Tezcan E (August 1, 1994) İnhibition of brain glutathione reductase by sodium arsenite. Marmara Medical Journal 7 3 115–118.
IEEE N. Açan and E. Tezcan, “İnhibition of brain glutathione reductase by sodium arsenite”, Marmara Med J, vol. 7, no. 3, pp. 115–118, 1994.
ISNAD Açan, N.l. - Tezcan, E.f. “İnhibition of Brain Glutathione Reductase by Sodium Arsenite”. Marmara Medical Journal 7/3 (August 1994), 115-118.
JAMA Açan N, Tezcan E. İnhibition of brain glutathione reductase by sodium arsenite. Marmara Med J. 1994;7:115–118.
MLA Açan, N.l. and E.f. Tezcan. “İnhibition of Brain Glutathione Reductase by Sodium Arsenite”. Marmara Medical Journal, vol. 7, no. 3, 1994, pp. 115-8.
Vancouver Açan N, Tezcan E. İnhibition of brain glutathione reductase by sodium arsenite. Marmara Med J. 1994;7(3):115-8.