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Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis

Year 1995, Volume: 8 Issue: 3, 136 - 139, 01.07.1995

I. B. Borlan M.M. Başaran S. Bilsel Y. Toga

Abstract

This study was undertaken to investigate the impact of Mycobacterium tuberculosis (M. tuberculosis) infection on in-vitro production of tumor necrosis

factor alpha (TNF-a), soluble CD8 (sCD8) and soluble IL-2 receptor (slL-2R) by peripheral blood mononuclear cells (PBMCs) from children with pulmonary tuberculosis (TB) versus controls.

PBMCs from 8 children with newly diagnosed pulmonary TB were stimulated with PHA and LPS either separately or in combination. Five healthy children and 4 adults were used as controls. Cell free supernatants were assayed for the presence of

TNF-a, sCD8 and slL-2R with a sandwich enzyme- linked immunosorbent assay (ELISA).

Although the median of TNF-a production was significantly higher in the unstimulated supernatants of patients (42.5pg/ml, 20.0-320.0) when compared to controls (15pg/ml, 0-90.0) (p<0.05) determination of sCD8 and slL-2R levels revealed no significant difference between the patients and controls.

Our findings indicated that PBMC obtained from children with TB spontaneously secreted high levels

of TNF-a which may exert immunoregulatory effects and thus mediate some of the clinical manifestations of tuberculosis.

Keywords

TNF-a sCD8 SİL-2R tuberculosis

References

  • Rook G AW, Al Attiyah R, Foley M. The role ol cytokines in the immunopathology of tuberculosis, and the regulation of agalactosyl IgG. Lymphokine Res 1989;8:323-328

Year 1995, Volume: 8 Issue: 3, 136 - 139, 01.07.1995

I. B. Borlan M.M. Başaran S. Bilsel Y. Toga

Abstract

References

  • Rook G AW, Al Attiyah R, Foley M. The role ol cytokines in the immunopathology of tuberculosis, and the regulation of agalactosyl IgG. Lymphokine Res 1989;8:323-328
There are 1 citations in total.

Details

Subjects Clinical Sciences
Journal Section Derleme
Authors

I. B. Borlan This is me

M.M. Başaran This is me

S. Bilsel This is me

Y. Toga This is me

Publication Date July 1, 1995
Published in Issue Year 1995 Volume: 8 Issue: 3

Cite

APA Borlan, I. B., Başaran, M., Bilsel, S., Toga, Y. (1995). Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis. Marmara Medical Journal, 8(3), 136-139.
AMA Borlan IB, Başaran M, Bilsel S, Toga Y. Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis. Marmara Med J. July 1995;8(3):136-139.
Chicago Borlan, I. B., M.M. Başaran, S. Bilsel, and Y. Toga. “Tnf-A, Scd8 and Sll-2r Levels in Pbmc Culture Supernatants of Children With Pulmonary Tuberculosis”. Marmara Medical Journal 8, no. 3 (July 1995): 136-39.
EndNote Borlan IB, Başaran M, Bilsel S, Toga Y (July 1, 1995) Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis. Marmara Medical Journal 8 3 136–139.
IEEE I. B. Borlan, M. Başaran, S. Bilsel, and Y. Toga, “Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis”, Marmara Med J, vol. 8, no. 3, pp. 136–139, 1995.
ISNAD Borlan, I. B. et al. “Tnf-A, Scd8 and Sll-2r Levels in Pbmc Culture Supernatants of Children With Pulmonary Tuberculosis”. Marmara Medical Journal 8/3 (July 1995), 136-139.
JAMA Borlan IB, Başaran M, Bilsel S, Toga Y. Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis. Marmara Med J. 1995;8:136–139.
MLA Borlan, I. B. et al. “Tnf-A, Scd8 and Sll-2r Levels in Pbmc Culture Supernatants of Children With Pulmonary Tuberculosis”. Marmara Medical Journal, vol. 8, no. 3, 1995, pp. 136-9.
Vancouver Borlan IB, Başaran M, Bilsel S, Toga Y. Tnf-a, scd8 and sll-2r levels in pbmc culture supernatants of children with pulmonary tuberculosis. Marmara Med J. 1995;8(3):136-9.