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Flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage of healthy persons

Year 1997, Volume: 10 Issue: 4, 193 - 197, 01.07.1997

Abstract

Objective: The nasal mucosal lymphocytes and nonlymphoid cells play a key role in the immune defence of the entire respiratory tract.

The purpose of this study is to detect lymphocytes and nonlymphoid cells in the nasal lavage of 25 healthy adult by flow cytometric analysis and compare them with the cells in the peripheral blood.

Methods: We studied 25 healthy volunteer students who did not have any upper respiratory diseases. Nasal Lavage was performed by instilling 8 ml of

0.9% sterile saline solution, 4 ml in each nostril with the subject head tilted 30° backwards. Then, lymphocytes and nonlymphoid cells obtained from the nasal lavages were analysed flow cytometrically.

Results: The flow cytometric analyses of nasal lavages were as follows: T helper cell/T suppressor cell ratio 2:1, total monoclear cell/monocyte ratio 11:1, T cell/B cell ratio 3.7:1, T cell/Natural killer cell ratio 5:1, T cell/active T cell ratio 5.8:1. The mean ratios were similar to the ratios of the peripheral blood (p<0.05).

Conclusion : Lymphoid and nonlymphoid cells which are present in the nasal mucosa play an important role in the inflammatory response and nasal lavage analysis reflects the immunoregulation of both nasal mucosa and peripheral blood. Therefore flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage is a very practical and an efficent technique in clinical practise.

References

  • Brad ding P, Feather IH, Wilson S, et al. Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitis subjects. J Immunol 1993; 151 ¡3853-3865.
Year 1997, Volume: 10 Issue: 4, 193 - 197, 01.07.1997

Abstract

References

  • Brad ding P, Feather IH, Wilson S, et al. Immunolocalization of cytokines in the nasal mucosa of normal and perennial rhinitis subjects. J Immunol 1993; 151 ¡3853-3865.
There are 1 citations in total.

Details

Subjects Clinical Sciences
Journal Section Review Makaleler
Authors

A. U. Lşık This is me

E. Seren This is me

Y. Tekelieğlu This is me

M. İmamoğlu This is me

Publication Date July 1, 1997
Published in Issue Year 1997 Volume: 10 Issue: 4

Cite

APA Lşık, A. U., Seren, E., Tekelieğlu, Y., İmamoğlu, M. (1997). Flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage of healthy persons. Marmara Medical Journal, 10(4), 193-197.
AMA Lşık AU, Seren E, Tekelieğlu Y, İmamoğlu M. Flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage of healthy persons. Marmara Med J. October 1997;10(4):193-197.
Chicago Lşık, A. U., E. Seren, Y. Tekelieğlu, and M. İmamoğlu. “Flow Cytometric Analysis of Lymphocytes and Nonlymphoid Cells in Nasal Lavage of Healthy Persons”. Marmara Medical Journal 10, no. 4 (October 1997): 193-97.
EndNote Lşık AU, Seren E, Tekelieğlu Y, İmamoğlu M (October 1, 1997) Flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage of healthy persons. Marmara Medical Journal 10 4 193–197.
IEEE A. U. Lşık, E. Seren, Y. Tekelieğlu, and M. İmamoğlu, “Flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage of healthy persons”, Marmara Med J, vol. 10, no. 4, pp. 193–197, 1997.
ISNAD Lşık, A. U. et al. “Flow Cytometric Analysis of Lymphocytes and Nonlymphoid Cells in Nasal Lavage of Healthy Persons”. Marmara Medical Journal 10/4 (October 1997), 193-197.
JAMA Lşık AU, Seren E, Tekelieğlu Y, İmamoğlu M. Flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage of healthy persons. Marmara Med J. 1997;10:193–197.
MLA Lşık, A. U. et al. “Flow Cytometric Analysis of Lymphocytes and Nonlymphoid Cells in Nasal Lavage of Healthy Persons”. Marmara Medical Journal, vol. 10, no. 4, 1997, pp. 193-7.
Vancouver Lşık AU, Seren E, Tekelieğlu Y, İmamoğlu M. Flow cytometric analysis of lymphocytes and nonlymphoid cells in nasal lavage of healthy persons. Marmara Med J. 1997;10(4):193-7.