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EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY

Year 1999, Volume: 12 Issue: 1, 33 - 38, 03.12.2016

Abstract

Objective: This study investigates the morphological effect of defibrotide on cold-immobilization stress- induced rat urinary bladder.
Material and Methods: Wistar albino rats were used in this study. After the cold, immobilization stress following the 48 hours of fasting, defibrotide was applied either intraperitonally (16 mg/ml) or intravesically (1200pg/ml). Urinary bladders were removed for light and electron microscopical investigations.
Results: The stress group showed leukocyte infiltration and edema, further vacuoles in the cytoplasm of urothelial cells and loose tight junctions. Additionally, urothelial protrusions towards the lumen were observed. The defibrotide-applied groups displayed regular morphology both under light and electron microscopy level.
Conclusion: These results showed that defibrotide reverse the stress induced morphological alterations. Although the biochemical effects of defibrotide is unknown, a possible explanation could be via modulation of calcium entry into the cells, and increase the permeability and regulation of inflammatory response and/or may have a role in the protection of urothelium by increasing the synthesis of prostaglandins and have a cytoprotective role in stress-induced bladder damage.
Key Words: Defibrotide, cytoprotection, cold- immobilization stress, urinary bladder, ultrastructure

References

  • Ader R, Cohen n, Felten D. Psychoneuroimmunology: interactions between the nervous system and the immune system. Lancet 1995;345:99-105.
  • Fiiller TA. Mechanisms of stress related mucosal damage. Am J Med 1987;83(suppl 6a):8-14.
  • Arbak S, Şen F, Ercan F, et af Stress ulcer: A
  • morphological approach. Marmara Med J
  • ; 7(4): 178-186.
  • Farber EM, Mickoloff BJ, Recht B, Fraki JE. Stress, symmtery and psoriasis: possible role of neuropeptides. J Am Ac Dermatol 1986; 14(2):305- 311.
  • Theoharides TC. Mast cells and migraines. Perspect Biol Med 1992;26:672-675.
  • Roziol JA, Clark DC, Oittes RF, Tan EM. The natural history of interstitial cystitis: A survey of 374 patients. J Urol 1993; 149:465-469.
  • Sant GR, Theoharides TC. The role of the mast cell in interstitial cystitis. Urol Clin north Am 1994;2 I (I ):4 I -53.
  • Spanos C, Pang X, Ligris K, et al. Stress-induced bladder mast cell activation: Implications for interstitial cytitis. J Urology 1997; 157:669-672.
  • Ercan F. Şan T. Çavdar S, et af Effect of experimental stress conditions on the urothelium, Xllth International symposium on morphological sciences, Thessaloniki, Greece, 22-27 September 1995:183-184.
  • Ercan F, Şan T, Aydın IT, Çavdar S. The evaluation of mast cells of rat urinary bladder under stress conditions. Marmara Med J 1997; 10(4): 183-192.
  • Gillenwater JY, Wein AJ. Summary of the national Institute of Arthritis, Diabetes, Digestive and Kidney Diseases Workshop on Interstitial Interstitial Cystitis, national Institutes of Health, Bethesda, Maryland, August 28-29. J Urol 1988, 140:203-206.
  • Patent (US) no. 3,770 (1973); 3,829,567 (1974); 3899481 (1975); Patent (British) no. 1,367,655 (1975).
  • Coccheri S, Biagi G, Legnani C, Bianchini B. Grauso F. Acute effects of defibrotide, an experimental antithrombotic agent, on fibrinolysis and blood
  • Marmara Medical Journal Volume 12 No: 1 January 1999
  • prostenoids in man. Eur J Clin Pharmacol 1988,35:151-156.
  • LöbeI P, Schrör K. Stimulation of vascular prostacyclin and inhibition of platelet function by oral defibrotide in cholesterol fed rabbits. Atherosclerosis 1989:80:69-79.
  • Palmer JK, Goa LK. Defibrotide: A review of its pharmacodynamic and pharmacokinetic properties, and therapeutic use in vascular disorders. Drugs 1993;45:259-294.
  • Berti F, Rissini G, Bianchi G, Alberico P, Tettamanti R, Calvani AB. Effect of defibrotide on prostacyclin releases from isolated rabbit kidneys and protection from post ishemic acute renal failtire in vivo. Eicosanoida 1991 ;4:209-215.
  • Biagi G, Legnani C, Rodariogo G, Coccheri S. Modulation of arachidonate metabolite generation in human blood by oral defibrotide. Arzneimittel Forschung 1991:41:511-515.
  • Bilsel S, Şan T, Erşahin Ç, Moini fl, Okar İ, Emerk K. Morphological changes in carotid arteries of rabbits induced by defibrotide infusion. Thromb Res 1994;76:433-440.
  • Covelli V. What is stress? flow does it correlate with the immune system? Ann MY Acad Sci 1994;741:212-218.
  • Di Perri T, Passini FL, Ceccatelli L, Pasqui AL, Capecchi PL. Defibrotide inhibits calcium dependent neutrophil activation: Implications for its pharmacological activity in vascular disorders. Angiology- J Vase Diseas 1991:971 -976.
  • Fleishmann JD, fluntley HM, Shingleton WB, Wentworth DB. Clinical and immunological response to nifedipine for the treatment of interstitial cystitis. J Urol 1991;146:1235-1239.
  • Fleischmann J. Calcium channel antagonists in the tretment of interstitial cystitis. Urol Clin Morth Am 1994; 21(1): 101-106.
  • Steers WD. Physiology of the urinary bladder. In:
  • Walsh PC, Retik AB, Vaughan ED, Wein AJ eds. Champell s Urology, eds. Philadelphia: W.B.
  • Saunders Com. 1992:142-175.
  • Saban R, Undem BJ, Keith IM, Saban MR, Tenglowski MW. Differential release of prostaglandins and leucotriens by sensitized guinea pig urinary bladder layers upon antigen challenge. J Urol 1994;152:544-554.
Year 1999, Volume: 12 Issue: 1, 33 - 38, 03.12.2016

Abstract

References

  • Ader R, Cohen n, Felten D. Psychoneuroimmunology: interactions between the nervous system and the immune system. Lancet 1995;345:99-105.
  • Fiiller TA. Mechanisms of stress related mucosal damage. Am J Med 1987;83(suppl 6a):8-14.
  • Arbak S, Şen F, Ercan F, et af Stress ulcer: A
  • morphological approach. Marmara Med J
  • ; 7(4): 178-186.
  • Farber EM, Mickoloff BJ, Recht B, Fraki JE. Stress, symmtery and psoriasis: possible role of neuropeptides. J Am Ac Dermatol 1986; 14(2):305- 311.
  • Theoharides TC. Mast cells and migraines. Perspect Biol Med 1992;26:672-675.
  • Roziol JA, Clark DC, Oittes RF, Tan EM. The natural history of interstitial cystitis: A survey of 374 patients. J Urol 1993; 149:465-469.
  • Sant GR, Theoharides TC. The role of the mast cell in interstitial cystitis. Urol Clin north Am 1994;2 I (I ):4 I -53.
  • Spanos C, Pang X, Ligris K, et al. Stress-induced bladder mast cell activation: Implications for interstitial cytitis. J Urology 1997; 157:669-672.
  • Ercan F. Şan T. Çavdar S, et af Effect of experimental stress conditions on the urothelium, Xllth International symposium on morphological sciences, Thessaloniki, Greece, 22-27 September 1995:183-184.
  • Ercan F, Şan T, Aydın IT, Çavdar S. The evaluation of mast cells of rat urinary bladder under stress conditions. Marmara Med J 1997; 10(4): 183-192.
  • Gillenwater JY, Wein AJ. Summary of the national Institute of Arthritis, Diabetes, Digestive and Kidney Diseases Workshop on Interstitial Interstitial Cystitis, national Institutes of Health, Bethesda, Maryland, August 28-29. J Urol 1988, 140:203-206.
  • Patent (US) no. 3,770 (1973); 3,829,567 (1974); 3899481 (1975); Patent (British) no. 1,367,655 (1975).
  • Coccheri S, Biagi G, Legnani C, Bianchini B. Grauso F. Acute effects of defibrotide, an experimental antithrombotic agent, on fibrinolysis and blood
  • Marmara Medical Journal Volume 12 No: 1 January 1999
  • prostenoids in man. Eur J Clin Pharmacol 1988,35:151-156.
  • LöbeI P, Schrör K. Stimulation of vascular prostacyclin and inhibition of platelet function by oral defibrotide in cholesterol fed rabbits. Atherosclerosis 1989:80:69-79.
  • Palmer JK, Goa LK. Defibrotide: A review of its pharmacodynamic and pharmacokinetic properties, and therapeutic use in vascular disorders. Drugs 1993;45:259-294.
  • Berti F, Rissini G, Bianchi G, Alberico P, Tettamanti R, Calvani AB. Effect of defibrotide on prostacyclin releases from isolated rabbit kidneys and protection from post ishemic acute renal failtire in vivo. Eicosanoida 1991 ;4:209-215.
  • Biagi G, Legnani C, Rodariogo G, Coccheri S. Modulation of arachidonate metabolite generation in human blood by oral defibrotide. Arzneimittel Forschung 1991:41:511-515.
  • Bilsel S, Şan T, Erşahin Ç, Moini fl, Okar İ, Emerk K. Morphological changes in carotid arteries of rabbits induced by defibrotide infusion. Thromb Res 1994;76:433-440.
  • Covelli V. What is stress? flow does it correlate with the immune system? Ann MY Acad Sci 1994;741:212-218.
  • Di Perri T, Passini FL, Ceccatelli L, Pasqui AL, Capecchi PL. Defibrotide inhibits calcium dependent neutrophil activation: Implications for its pharmacological activity in vascular disorders. Angiology- J Vase Diseas 1991:971 -976.
  • Fleishmann JD, fluntley HM, Shingleton WB, Wentworth DB. Clinical and immunological response to nifedipine for the treatment of interstitial cystitis. J Urol 1991;146:1235-1239.
  • Fleischmann J. Calcium channel antagonists in the tretment of interstitial cystitis. Urol Clin Morth Am 1994; 21(1): 101-106.
  • Steers WD. Physiology of the urinary bladder. In:
  • Walsh PC, Retik AB, Vaughan ED, Wein AJ eds. Champell s Urology, eds. Philadelphia: W.B.
  • Saunders Com. 1992:142-175.
  • Saban R, Undem BJ, Keith IM, Saban MR, Tenglowski MW. Differential release of prostaglandins and leucotriens by sensitized guinea pig urinary bladder layers upon antigen challenge. J Urol 1994;152:544-554.
There are 30 citations in total.

Details

Journal Section Original Research
Authors

Feriha Ercan This is me

Tangül San This is me

Safiye Çavdar This is me

Oya Karabulut This is me

Publication Date December 3, 2016
Published in Issue Year 1999 Volume: 12 Issue: 1

Cite

APA Ercan, F., San, T., Çavdar, S., Karabulut, O. (2016). EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY. Marmara Medical Journal, 12(1), 33-38.
AMA Ercan F, San T, Çavdar S, Karabulut O. EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY. Marmara Med J. June 2016;12(1):33-38.
Chicago Ercan, Feriha, Tangül San, Safiye Çavdar, and Oya Karabulut. “EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY”. Marmara Medical Journal 12, no. 1 (June 2016): 33-38.
EndNote Ercan F, San T, Çavdar S, Karabulut O (June 1, 2016) EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY. Marmara Medical Journal 12 1 33–38.
IEEE F. Ercan, T. San, S. Çavdar, and O. Karabulut, “EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY”, Marmara Med J, vol. 12, no. 1, pp. 33–38, 2016.
ISNAD Ercan, Feriha et al. “EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY”. Marmara Medical Journal 12/1 (June 2016), 33-38.
JAMA Ercan F, San T, Çavdar S, Karabulut O. EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY. Marmara Med J. 2016;12:33–38.
MLA Ercan, Feriha et al. “EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY”. Marmara Medical Journal, vol. 12, no. 1, 2016, pp. 33-38.
Vancouver Ercan F, San T, Çavdar S, Karabulut O. EFFECTS OF DEFIBROTIDE ON STRESS-INDUCED BLADDER MORPHOLOGY. Marmara Med J. 2016;12(1):33-8.