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THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL

Year 2002, Volume: 15 Issue: 2, 79 - 85, 03.12.2016

Abstract

Objective: "Genetic Absence Epilepsy Rats from
Strasbourg" (GAERS), an inbred Wistar strain,
serve as an experimental venue. These rats
generate spontaneous spike-and-wave
discharges (SWD) and have increased yaminobutyric
acid (GABA) levels in the
ventrolateral thalamus (VLT). Recently,
substantia nigra pars reticulata (SNpr) was
reported to act as an endogeneous inhibitory
mechanism in the generation, onset and
maintenance of various types of seizures. The
presence of tonic control exerted by SNpr in
absence seizures should also be tested in
GAERS.
Methods: In this current study, GABA and Lglutamic
acid release in VLT of GAERS with
partial bilateral electrolytic lesions of SNpr was
evaluated by using microdialysis technique with
fluorescent detection.
Results: GABA levels in VLT were 0.12±0.04 pM
and 0.24±0.08 pM in sham-lesioned and SNprlesioned
GAERS, respectively. L-glutamic acid
level was found to be 0.415±0.150 pM in shamlesioned
group and 0.324±0.094 pM in SNprlesioned
GAERS. Statistical analysis revealed no
significant difference between sham-lesioned
and SNpr-lesioned rats. The number and the
duration of SWD were also similar in two groups.
Conclusion: These findings show that SNpr
does not exert a tonic control in GAERS and we
assume that intact SNpr acts as a site that may
exert an inhibition on target structures when
activated in GAERS.
Key Words: GAERS (Genetic Absence
Epilepsy Rats from Strasbourg), GABA (yaminobutyric
acid), Substantia nigra,
Ventrolateral thalamus, Absence epilepsy,
Microdialysis, Nigral control of epilepsy.

References

  • ladorola MJ, Gale li. Substantia nigra: site of anticonvulsant activity mediated by GABA. Science 1982; 218: 1237-1240.
  • Gale R. Mechanisms of seizure control mediated by gamma-aminobutyric acid: role of the substantia nigra. Fed Froc 1985; 44: 2414-2424.
  • Depaulis A, Vergnes M, Marescaux C Endogeneous control of epilepsy-the nigral output pathways in the inhibitory control of substantia nigra over generalized non- convulsive seizures in the rat. Neuroscience 1990; 39: 339-349.
  • Depaulis A, Vergnes M, Liu Z, hem pi Z, Marescaux C. Involvement of nigral control of epilepsy -the nigral inhibitory system. Frog Neurobiol 1994; 42: 33-52.
  • Löscher W, Czuczwar S, Jäckel R, Schwarz M. Effect of microinjections of y-vinyI GABA or isoniazid into substantia nigra on the
  • development of amygdala kindling in rats. Exp Neurol 1987; 95:622-638.
  • Depaulis A, Vergnes M, Marescaux C, Lannes B, Warier JM. Evidence that activation of GABA receptors in the substantia nigra supresses spontaneous spike and wave discharges in the rat. Brain Res 1988; 448:
  • -29.
  • Nauta WJfi, Domesick VB. Afferent and efferent relationships of the basal ganglia. ln:Evered and O'Connor M, eds. Functions of the basal ganglia. London: CIBA Foundation Symposium 107, Pitman, 1985:3-23.
  • Chevalier G, Vascher S, Deniali JM, Desban M. Disinhibition as a basic process in the expression of striatal functions-!. The striatonigral influence on tecto- spinal/tectodiencephalic neurons. Brain Res 1985:334: 215-226.
  • Oertel WH, Scmechel DE, Brownstein MJ, Tappaz ML, Ransom Dll, Roprin IJ . Decrease of glutamate decarboxylase (GAD)- immunoreactive nerve terminals In the substantia nigra after kainic acid lesion of striatum. J flistochem and Cytochem 1981; 29: 977-980.
  • Marescaux C, Vergnes M, Depaulis A . Genetic absence epilepsy rats from Strasbourg- a review. J Neural Transmission 1992; (Suppl. 35): 179-188.
  • Vergnes M, Marescaux C, Depaulis A, Micheletti G, Waiter JM . Spontaneous spike and wave discharges in thalamus and cortex in a rat model of genetic petit mal-like seizures. Exp Neurol 1987; 96: 127-136.
  • Liu Z, Marescaux C, Vergnes M, Depaulis A . Evidence for a critical role of GABA'ergic transmission within the thalamus in the genesis and control of absence seizures in the rat. Brain Res 1991; 45: 1-7.
  • Richards DA, Lemos T, Whitton FS, Bowery NG. Extracellular GABA in the ventrolateral thalamus of rats exhibiting absence epilepsy: a microdialysis study. J Neurochem 1995; 65(4): 1674-1680.
  • Faxinos G, Watson C. The rat brain in stereotaxic coordinates, 2nd edition, London: Academic Press, 1986; 29-44.
  • Obrenovitch TP, Richards DA, Sarna GS, Symon L. Combined intracerebral microdialysis and electrophysiological recording: methodology and applications. J Neurosci Methods 1995; 47: 139-145.
  • Biggs CS, Pearce BR, Fowler LJ, Whitton PS. The effect of sodium valproate on
  • Partial bilateral lesioning of substantia nigra
  • extracellular GABA and other amino acids in the rat ventral hippocampus: an in vivo microdialysis study. Brain Res 1992; 594: 138-142.
  • I 7. Smith S, Sharp T. Measurement of GABA in rat brain microdialysates using
  • ophtaldealdehyde-sulphite derivatization and high-performance liquid chromatography with electrochemical detection. J Chromatogr 1994; 652: 228-233.
  • Alexander G, Crutcher M. functional architecture of basal ganglia circuits: neural substrates of parallel processing. Trends neurosci 1990; 13: 266-271.
  • Deransart C, Vercueil L, Marescaux C, Depaulis A. The role of basal ganglia in the control of generalized absence seizures. Epilepsy Res 1998; 32: 213-223.
  • fallon JFt, Loughlin SE . Substantia nigra. In: Paxinos G, ed. The Rat nervous System, new York : Academic Press, 1995; 215-238.
  • tleimer L, Zahm D, Alheid G. Basal ganglia In: Paxinos G, eds. The Rat nervous System, new York : Academic Press, 1995: 579-628.
  • Morari M, Oconnor IVT, Ungerstedt U, Bianchi C, Fuxe R. Functional neuroanatomy of nigrostriatal and striatonigral pathways as studied with dual probe microdialysis in the awake rat. 2. Evidence for striatal n-methyl-D- aspartate receptor regulation of striatonigral gabaergic transmission and motor function, neuroscience 1996; 72: 89-97.
  • Shin C, Silver JM, Bonhaus DW, Mcnamara
  • JO. The role of substantia nigra in he development of kindling: pharmacologic and lesion studies. Brain Res 1987; 412: 311-
  • Goren MZ, Richards DA, Turner FI, Bowery nG. Extracellular GABA levels are increased in brain regions associated with the generation of absence seizures. Br J Pharmacol 1997; 122: 83P.
  • Corodimas KP, Rosenblat JS, Matthews TM, Morell Jl. neuroanatomical tract racing provides histological verification of neuron loss following cytotoxic lesion. J neurosci Methods 1994; 56: 71-75.
Year 2002, Volume: 15 Issue: 2, 79 - 85, 03.12.2016

Abstract

References

  • ladorola MJ, Gale li. Substantia nigra: site of anticonvulsant activity mediated by GABA. Science 1982; 218: 1237-1240.
  • Gale R. Mechanisms of seizure control mediated by gamma-aminobutyric acid: role of the substantia nigra. Fed Froc 1985; 44: 2414-2424.
  • Depaulis A, Vergnes M, Marescaux C Endogeneous control of epilepsy-the nigral output pathways in the inhibitory control of substantia nigra over generalized non- convulsive seizures in the rat. Neuroscience 1990; 39: 339-349.
  • Depaulis A, Vergnes M, Liu Z, hem pi Z, Marescaux C. Involvement of nigral control of epilepsy -the nigral inhibitory system. Frog Neurobiol 1994; 42: 33-52.
  • Löscher W, Czuczwar S, Jäckel R, Schwarz M. Effect of microinjections of y-vinyI GABA or isoniazid into substantia nigra on the
  • development of amygdala kindling in rats. Exp Neurol 1987; 95:622-638.
  • Depaulis A, Vergnes M, Marescaux C, Lannes B, Warier JM. Evidence that activation of GABA receptors in the substantia nigra supresses spontaneous spike and wave discharges in the rat. Brain Res 1988; 448:
  • -29.
  • Nauta WJfi, Domesick VB. Afferent and efferent relationships of the basal ganglia. ln:Evered and O'Connor M, eds. Functions of the basal ganglia. London: CIBA Foundation Symposium 107, Pitman, 1985:3-23.
  • Chevalier G, Vascher S, Deniali JM, Desban M. Disinhibition as a basic process in the expression of striatal functions-!. The striatonigral influence on tecto- spinal/tectodiencephalic neurons. Brain Res 1985:334: 215-226.
  • Oertel WH, Scmechel DE, Brownstein MJ, Tappaz ML, Ransom Dll, Roprin IJ . Decrease of glutamate decarboxylase (GAD)- immunoreactive nerve terminals In the substantia nigra after kainic acid lesion of striatum. J flistochem and Cytochem 1981; 29: 977-980.
  • Marescaux C, Vergnes M, Depaulis A . Genetic absence epilepsy rats from Strasbourg- a review. J Neural Transmission 1992; (Suppl. 35): 179-188.
  • Vergnes M, Marescaux C, Depaulis A, Micheletti G, Waiter JM . Spontaneous spike and wave discharges in thalamus and cortex in a rat model of genetic petit mal-like seizures. Exp Neurol 1987; 96: 127-136.
  • Liu Z, Marescaux C, Vergnes M, Depaulis A . Evidence for a critical role of GABA'ergic transmission within the thalamus in the genesis and control of absence seizures in the rat. Brain Res 1991; 45: 1-7.
  • Richards DA, Lemos T, Whitton FS, Bowery NG. Extracellular GABA in the ventrolateral thalamus of rats exhibiting absence epilepsy: a microdialysis study. J Neurochem 1995; 65(4): 1674-1680.
  • Faxinos G, Watson C. The rat brain in stereotaxic coordinates, 2nd edition, London: Academic Press, 1986; 29-44.
  • Obrenovitch TP, Richards DA, Sarna GS, Symon L. Combined intracerebral microdialysis and electrophysiological recording: methodology and applications. J Neurosci Methods 1995; 47: 139-145.
  • Biggs CS, Pearce BR, Fowler LJ, Whitton PS. The effect of sodium valproate on
  • Partial bilateral lesioning of substantia nigra
  • extracellular GABA and other amino acids in the rat ventral hippocampus: an in vivo microdialysis study. Brain Res 1992; 594: 138-142.
  • I 7. Smith S, Sharp T. Measurement of GABA in rat brain microdialysates using
  • ophtaldealdehyde-sulphite derivatization and high-performance liquid chromatography with electrochemical detection. J Chromatogr 1994; 652: 228-233.
  • Alexander G, Crutcher M. functional architecture of basal ganglia circuits: neural substrates of parallel processing. Trends neurosci 1990; 13: 266-271.
  • Deransart C, Vercueil L, Marescaux C, Depaulis A. The role of basal ganglia in the control of generalized absence seizures. Epilepsy Res 1998; 32: 213-223.
  • fallon JFt, Loughlin SE . Substantia nigra. In: Paxinos G, ed. The Rat nervous System, new York : Academic Press, 1995; 215-238.
  • tleimer L, Zahm D, Alheid G. Basal ganglia In: Paxinos G, eds. The Rat nervous System, new York : Academic Press, 1995: 579-628.
  • Morari M, Oconnor IVT, Ungerstedt U, Bianchi C, Fuxe R. Functional neuroanatomy of nigrostriatal and striatonigral pathways as studied with dual probe microdialysis in the awake rat. 2. Evidence for striatal n-methyl-D- aspartate receptor regulation of striatonigral gabaergic transmission and motor function, neuroscience 1996; 72: 89-97.
  • Shin C, Silver JM, Bonhaus DW, Mcnamara
  • JO. The role of substantia nigra in he development of kindling: pharmacologic and lesion studies. Brain Res 1987; 412: 311-
  • Goren MZ, Richards DA, Turner FI, Bowery nG. Extracellular GABA levels are increased in brain regions associated with the generation of absence seizures. Br J Pharmacol 1997; 122: 83P.
  • Corodimas KP, Rosenblat JS, Matthews TM, Morell Jl. neuroanatomical tract racing provides histological verification of neuron loss following cytotoxic lesion. J neurosci Methods 1994; 56: 71-75.
There are 31 citations in total.

Details

Journal Section Original Research
Authors

Zafer Gören This is me

Rezzan Aker This is me

Filiz Onat This is me

Aydan Ergün This is me

Publication Date December 3, 2016
Published in Issue Year 2002 Volume: 15 Issue: 2

Cite

APA Gören, Z., Aker, R., Onat, F., Ergün, A. (2016). THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL. Marmara Medical Journal, 15(2), 79-85.
AMA Gören Z, Aker R, Onat F, Ergün A. THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL. Marmara Med J. March 2016;15(2):79-85.
Chicago Gören, Zafer, Rezzan Aker, Filiz Onat, and Aydan Ergün. “THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL”. Marmara Medical Journal 15, no. 2 (March 2016): 79-85.
EndNote Gören Z, Aker R, Onat F, Ergün A (March 1, 2016) THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL. Marmara Medical Journal 15 2 79–85.
IEEE Z. Gören, R. Aker, F. Onat, and A. Ergün, “THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL”, Marmara Med J, vol. 15, no. 2, pp. 79–85, 2016.
ISNAD Gören, Zafer et al. “THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL”. Marmara Medical Journal 15/2 (March 2016), 79-85.
JAMA Gören Z, Aker R, Onat F, Ergün A. THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL. Marmara Med J. 2016;15:79–85.
MLA Gören, Zafer et al. “THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL”. Marmara Medical Journal, vol. 15, no. 2, 2016, pp. 79-85.
Vancouver Gören Z, Aker R, Onat F, Ergün A. THE EFFECTS OF PARTIAL BILATERAL LESIONING OF SUBSTANTIA NIGRA IN A GENETIC ABSENCE EPILEPSY RAT MODEL. Marmara Med J. 2016;15(2):79-85.