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Year 2015, Volume 28 , Issue 1, Pages 13 - 20 2015-07-02

Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi

Gülnaz KERVANCIOĞLU [1] , Gülnaz KERVANCIOĞLU [2] , Şule ÇETİNEL [3] , Şule ÇETİNEL [4] , Elif KERVANCIOĞLU DEMİRCİ [5] , Elif KERVANCIOĞLU DEMİRCİ [6] , Gülçin EKTER KANTER [7] , Gülçin EKTER KANTER [8] , Ertan KERVANCIOĞLU [9] , M. Ertan KERVANCIOĞLU [10]

Abstract

Objective: The cryopreservation of prepubertal testicular tissue promises to preserve fertility in boys under cancer treatment. The effects of a seeding process was investigated using different carriers for prepubertal testicular cell suspensions in order to develop an appropriate method for cryopreservation. Materials and Methods: Testicular cell suspensions of 7-8 day old male Wistar rats were frozen by a programmed slow freezing method. The straw and cryotube selected as carriers underwent a seeding process. The morphological effects on spermatogonial stem cells and Sertoli cells were evaluated semi-quantitatively by light microscopy and qualitatively with a transmission electron microscope. Results: The rate of vitality with or without seeding in straw was respectively, 90.6% (±3.0), 89.4 (±1.3) and in cryotube 77.6 (±4.2) and 85.4 (±2.7). The seeding process, showed no significant difference between the straw groups, but a significant difference was seen between the cryotube groups (p<0.0001). The nuclei, cytoplasmic structures and membranes of Sertoli and Conclusion: In this study, we compared two carriers to observe the effect of seeding during cryopreservation of prepubertal testicular cell suspensions and found that seeding in straw was the preferable
Amaç: Prepubertal testis kriyoprezervasyonu, kanser tedavisi yapılacak erkek çocuklarda fertilitenin korunması için günümüz teknolojisinde umut vadetmektedir. Kriyoprezervasyonda en uygun yöntemin geliştirilmesi için prepubertal testis hücre süspansiyonunda farklı taşıyıcılar kullanarak seeding etkisi araştırıldı. Gereç ve Yöntem: Yedi-sekiz günlük Wistar yavru erkek sıçan testis hücre süspansiyonları, programlı yavaş dondurma yöntemiyle donduruldu. Taşıyıcı olarak seçilen yüksek güvenlikli straw ve kriyotüpe seeding uygulandı. Spermatogonyal kök hücreler ve Sertoli hücreleri üzerindeki morfolojik etki, semi-kantitatif olarak ışık, kalitatif olarak geçirimli elektron mikroskobunda incelendi. Bulgular: Canlılık oranı, yüksek güvenlikli straw’da seeding uygulanmayan ve uygulanan gruplarda sırasıyla %90,6±3,0, %89,4±1,3, kriyotüpte %77,6±4,2 ve %85,4±2,7 bulundu. Seeding uygulanmasıyla, canlılık oranında straw grupları arasında fark görülmemesine rağmen, kriyotüp grupları arasında fark anlamlıydı (p

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Details

Primary Language tr
Journal Section Articles
Authors

Author: Gülnaz KERVANCIOĞLU

Author: Gülnaz KERVANCIOĞLU

Author: Şule ÇETİNEL

Author: Şule ÇETİNEL

Author: Elif KERVANCIOĞLU DEMİRCİ

Author: Elif KERVANCIOĞLU DEMİRCİ

Author: Gülçin EKTER KANTER

Author: Gülçin EKTER KANTER

Author: Ertan KERVANCIOĞLU

Author: M. Ertan KERVANCIOĞLU
Dates

Publication Date : July 2, 2015

Cite

Bibtex @ { marumj3162, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {}, publisher = {Marmara University}, year = {2015}, volume = {28}, pages = {13 - 20}, doi = {10.5472/MMJ.2015.03894.1}, title = {Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi}, key = {cite}, author = {Kervancıoğlu, Gülnaz and Çeti̇nel, Şule and Kervancıoğlu Demi̇rci̇, Elif and Ekter Kanter, Gülçin and Kervancıoğlu, M. Ertan} }
APA Kervancıoğlu, G , Kervancıoğlu, G , Çeti̇nel, Ş , Çeti̇nel, Ş , Kervancıoğlu Demi̇rci̇, E , Kervancıoğlu Demi̇rci̇, E , Ekter Kanter, G , Ekter Kanter, G , Kervancıoğlu, E , Kervancıoğlu, M . (2015). Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi . Marmara Medical Journal , 28 (1) , 13-20 . DOI: 10.5472/MMJ.2015.03894.1
MLA Kervancıoğlu, G , Kervancıoğlu, G , Çeti̇nel, Ş , Çeti̇nel, Ş , Kervancıoğlu Demi̇rci̇, E , Kervancıoğlu Demi̇rci̇, E , Ekter Kanter, G , Ekter Kanter, G , Kervancıoğlu, E , Kervancıoğlu, M . "Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi" . Marmara Medical Journal 28 (2015 ): 13-20 <https://dergipark.org.tr/en/pub/marumj/issue/429/3162>
Chicago Kervancıoğlu, G , Kervancıoğlu, G , Çeti̇nel, Ş , Çeti̇nel, Ş , Kervancıoğlu Demi̇rci̇, E , Kervancıoğlu Demi̇rci̇, E , Ekter Kanter, G , Ekter Kanter, G , Kervancıoğlu, E , Kervancıoğlu, M . "Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi". Marmara Medical Journal 28 (2015 ): 13-20
RIS TY - JOUR T1 - Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi AU - Gülnaz Kervancıoğlu , Gülnaz Kervancıoğlu , Şule Çeti̇nel , Şule Çeti̇nel , Elif Kervancıoğlu Demi̇rci̇ , Elif Kervancıoğlu Demi̇rci̇ , Gülçin Ekter Kanter , Gülçin Ekter Kanter , Ertan Kervancıoğlu , M. Ertan Kervancıoğlu Y1 - 2015 PY - 2015 N1 - doi: 10.5472/MMJ.2015.03894.1 DO - 10.5472/MMJ.2015.03894.1 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 13 EP - 20 VL - 28 IS - 1 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/MMJ.2015.03894.1 UR - https://doi.org/10.5472/MMJ.2015.03894.1 Y2 - 2021 ER -
EndNote %0 Marmara Medical Journal Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi %A Gülnaz Kervancıoğlu , Gülnaz Kervancıoğlu , Şule Çeti̇nel , Şule Çeti̇nel , Elif Kervancıoğlu Demi̇rci̇ , Elif Kervancıoğlu Demi̇rci̇ , Gülçin Ekter Kanter , Gülçin Ekter Kanter , Ertan Kervancıoğlu , M. Ertan Kervancıoğlu %T Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi %D 2015 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 28 %N 1 %R doi: 10.5472/MMJ.2015.03894.1 %U 10.5472/MMJ.2015.03894.1
ISNAD Kervancıoğlu, Gülnaz , Kervancıoğlu, Gülnaz , Çeti̇nel, Şule , Çeti̇nel, Şule , Kervancıoğlu Demi̇rci̇, Elif , Kervancıoğlu Demi̇rci̇, Elif , Ekter Kanter, Gülçin , Ekter Kanter, Gülçin , Kervancıoğlu, Ertan , Kervancıoğlu, M. Ertan . "Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi". Marmara Medical Journal 28 / 1 (July 2015): 13-20 . https://doi.org/10.5472/MMJ.2015.03894.1
AMA Kervancıoğlu G , Kervancıoğlu G , Çeti̇nel Ş , Çeti̇nel Ş , Kervancıoğlu Demi̇rci̇ E , Kervancıoğlu Demi̇rci̇ E , Ekter Kanter G , Ekter Kanter G , Kervancıoğlu E , Kervancıoğlu M . Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi. Marmara Med J. 2015; 28(1): 13-20.
Vancouver Kervancıoğlu G , Kervancıoğlu G , Çeti̇nel Ş , Çeti̇nel Ş , Kervancıoğlu Demi̇rci̇ E , Kervancıoğlu Demi̇rci̇ E , Ekter Kanter G , Ekter Kanter G , Kervancıoğlu E , Kervancıoğlu M . Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi. Marmara Medical Journal. 2015; 28(1): 13-20.
IEEE G. Kervancıoğlu , G. Kervancıoğlu , Ş. Çeti̇nel , Ş. Çeti̇nel , E. Kervancıoğlu Demi̇rci̇ , E. Kervancıoğlu Demi̇rci̇ , G. Ekter Kanter , G. Ekter Kanter , E. Kervancıoğlu and M. Kervancıoğlu , "Prepubertal testis hücre süspansiyonun dondurulmasında seeding etkisi", Marmara Medical Journal, vol. 28, no. 1, pp. 13-20, Jul. 2015, doi:10.5472/MMJ.2015.03894.1
Authors of the Article
Gülnaz KERVANCIOĞLU [1]
Gülnaz KERVANCIOĞLU [2]
Şule ÇETİNEL [3]
Şule ÇETİNEL [4]
Elif KERVANCIOĞLU DEMİRCİ [5]
Elif KERVANCIOĞLU DEMİRCİ [6]
Gülçin EKTER KANTER [7]
Gülçin EKTER KANTER [8]
Ertan KERVANCIOĞLU [9]
M. Ertan KERVANCIOĞLU [10]
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