Year 2020, Volume 33 , Issue 1, Pages 7 - 16 2020-01-31

Dental follicle mesenchymal stem cells regulate responses in sepsis

Fethi GUL [1] , Deniz GENC [2] , Mustafa Kemal ARSLANTAS [3] , Noushin ZIBANDEH [4] , Leyla TOPCU [5] , Tunç AKKOC [6] , İsmail CINEL [7]


Objective: Sepsis-induced immune alterations are associated with secondary infections and increased risk of death. The use of mesenchymal stem cells (MSCs) has been described as a novel therapeutic strategy. We evaluated the immunomodulatory effects of human dental follicle (DF-MSCs) on lymphocytes of sepsis and septic shock patients. Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood samples of sepsis, septic shock and healthy subjects. PBMCs were co-cultured in the presence and absence of DF-MSCs with or without interferon-gamma (IFN-γ) for 72 hours. CD4+CD25+FoxP3+regulatory T (Treg) cell frequency, lymphocyte proliferation, cytokine levels and apoptosis were evaluated via flow cytometry. Results: DF-MSCs significantly suppressed proliferation of lymphocytes in sepsis group compared to septic shock group (p<0.005). DF-MSCs remarkably increased Treg ratio in sepsis compared to control group (p<0.05). Reduction of lymphocyte apoptosis in cocultures of DF-MSCs and PBMC was significant in both sepsis and septic shock groups. IFN-γ stimulation of DF-MSCs ameliorated shift in the T-cell subsets from Th2 to Th1 phenotype in septic shock. Conclusion: Our findings revealed that DF-MSCs have immunoregulatory effects both in sepsis and septic shock, by reducing interleukin-4 (IL-4) and increasing IFN-γ levels. This immunoreactivity regulation may open new therapeutic approaches for septic shock patients.
Sepsis, Septic shock, Dental follicle mesenchymal stem cells, Immunomodulation
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Primary Language en
Subjects Medicine
Journal Section Original Research
Authors

Author: Fethi GUL
Institution: Marmara University Training and Research Hospital
Country: Turkey


Author: Deniz GENC
Institution: Marmara University Training and Research Hospital
Country: Turkey


Author: Mustafa Kemal ARSLANTAS
Institution: Marmara University Training and Research Hospital
Country: Turkey


Author: Noushin ZIBANDEH
Institution: Marmara University Training and Research Hospital
Country: Turkey


Author: Leyla TOPCU
Institution: Marmara University Training and Research Hospital
Country: Turkey


Author: Tunç AKKOC
Institution: Marmara University Training and Research Hospital
Country: Turkey


Author: İsmail CINEL (Primary Author)
Institution: Marmara University Training and Research Hospital
Country: Turkey


Dates

Publication Date : January 31, 2020

Bibtex @research article { marumj681996, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {}, publisher = {Marmara University}, year = {2020}, volume = {33}, pages = {7 - 16}, doi = {10.5472/marumj.681996}, title = {Dental follicle mesenchymal stem cells regulate responses in sepsis}, key = {cite}, author = {GUL, Fethi and GENC, Deniz and ARSLANTAS, Mustafa Kemal and ZIBANDEH, Noushin and TOPCU, Leyla and AKKOC, Tunç and CINEL, İsmail} }
APA GUL, F , GENC, D , ARSLANTAS, M , ZIBANDEH, N , TOPCU, L , AKKOC, T , CINEL, İ . (2020). Dental follicle mesenchymal stem cells regulate responses in sepsis. Marmara Medical Journal , 33 (1) , 7-16 . DOI: 10.5472/marumj.681996
MLA GUL, F , GENC, D , ARSLANTAS, M , ZIBANDEH, N , TOPCU, L , AKKOC, T , CINEL, İ . "Dental follicle mesenchymal stem cells regulate responses in sepsis". Marmara Medical Journal 33 (2020 ): 7-16 <https://dergipark.org.tr/en/pub/marumj/issue/52168/681996>
Chicago GUL, F , GENC, D , ARSLANTAS, M , ZIBANDEH, N , TOPCU, L , AKKOC, T , CINEL, İ . "Dental follicle mesenchymal stem cells regulate responses in sepsis". Marmara Medical Journal 33 (2020 ): 7-16
RIS TY - JOUR T1 - Dental follicle mesenchymal stem cells regulate responses in sepsis AU - Fethi GUL , Deniz GENC , Mustafa Kemal ARSLANTAS , Noushin ZIBANDEH , Leyla TOPCU , Tunç AKKOC , İsmail CINEL Y1 - 2020 PY - 2020 N1 - doi: 10.5472/marumj.681996 DO - 10.5472/marumj.681996 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 7 EP - 16 VL - 33 IS - 1 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/marumj.681996 UR - https://doi.org/10.5472/marumj.681996 Y2 - 2019 ER -
EndNote %0 Marmara Medical Journal Dental follicle mesenchymal stem cells regulate responses in sepsis %A Fethi GUL , Deniz GENC , Mustafa Kemal ARSLANTAS , Noushin ZIBANDEH , Leyla TOPCU , Tunç AKKOC , İsmail CINEL %T Dental follicle mesenchymal stem cells regulate responses in sepsis %D 2020 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 33 %N 1 %R doi: 10.5472/marumj.681996 %U 10.5472/marumj.681996
ISNAD GUL, Fethi , GENC, Deniz , ARSLANTAS, Mustafa Kemal , ZIBANDEH, Noushin , TOPCU, Leyla , AKKOC, Tunç , CINEL, İsmail . "Dental follicle mesenchymal stem cells regulate responses in sepsis". Marmara Medical Journal 33 / 1 (January 2020): 7-16 . https://doi.org/10.5472/marumj.681996
AMA GUL F , GENC D , ARSLANTAS M , ZIBANDEH N , TOPCU L , AKKOC T , CINEL İ . Dental follicle mesenchymal stem cells regulate responses in sepsis. Marmara Med J. 2020; 33(1): 7-16.
Vancouver GUL F , GENC D , ARSLANTAS M , ZIBANDEH N , TOPCU L , AKKOC T , CINEL İ . Dental follicle mesenchymal stem cells regulate responses in sepsis. Marmara Medical Journal. 2020; 33(1): 16-7.