Year 2020, Volume 33 , Issue 3, Pages 113 - 118 2020-10-23

Examination of the apoptotic effects of betulinic acid on renal cancer cell lines

Arzu ERGEN [1] , E. Sinem IPLIK [2] , Baris ERTUGRUL [3] , Merve Nur ATAS [4] , Goksu KASARCI [5] , Bedia CAKMAKOGLU [6]


Objective: Renal cancers are highly resistant to standard hormonal therapy, radiotherapy, and chemotherapy, and the survival rates are extremely low. Betulinic acid is a pentasilic triterpenoid saponin of lupine type obtained from various natural plants, especially from the shell of Betula plant. Betulinic acid was shown both in in vivo, and in vitro to have the ability to induce apoptotic pathways causing no toxicity for normal cells, and also has immunomodulatory effects. The aim of the present project is to investigate the anticancer effects of betulinic acid on CAKI-2 (ATCC® HTB-47™; clear cell renal carcinoma), ACHN (ATCC® CRL-1611™; renal cell adenocarcinoma) and MRC-5 (ATCC® CCL-171™: normal lung fibroblast) cell lines. Materials and Methods: The dose, and time-dependent cell viability was determined using the WST-1 test first in cell lines, and then apoptotic activity was determined with Annexin-V, apoptosis related nucleosomal enrichment factor levels, and Caspase 3 / BCA activity. Results: Betulinic acid reduced the CAKI-2, and ACHN cell viability in dose, and time-dependent manner inducing the apoptotic pathway. Conclusion: Researchers in the present study concluded in accordance with the results of Annexin-V, apoptosis-associated nucleosomal enrichment factor levels and Caspase 3 / BCA activity that betulinic acid triggered the apoptosis in both renal cancer cell lines, especially by the Caspase 3 activity.
Renal cancer, Betulinic acid, Apoptosis, ACHN, CAKI-2
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Primary Language en
Subjects Medicine
Journal Section Articles
Authors

Orcid: 0000-0001-5736-8453
Author: Arzu ERGEN
Institution: Marmara University
Country: Turkey


Orcid: 0000-0003-3465-1808
Author: E. Sinem IPLIK
Institution: YENI YUZYIL UNIVERSITY
Country: Turkey


Orcid: 0000-0003-3878-1829
Author: Baris ERTUGRUL
Institution: Marmara University
Country: Turkey


Orcid: 0000-0001-5045-5095
Author: Merve Nur ATAS
Institution: Marmara University
Country: Turkey


Orcid: 0000-0001-9766-4361
Author: Goksu KASARCI
Institution: Marmara University
Country: Turkey


Orcid: 0000-0001-7960-9131
Author: Bedia CAKMAKOGLU
Institution: Marmara University
Country: Turkey


Dates

Publication Date : October 23, 2020

Bibtex @research article { marumj815542, journal = {Marmara Medical Journal}, issn = {1019-1941}, eissn = {1309-9469}, address = {}, publisher = {Marmara University}, year = {2020}, volume = {33}, pages = {113 - 118}, doi = {10.5472/marumj.815542}, title = {Examination of the apoptotic effects of betulinic acid on renal cancer cell lines}, key = {cite}, author = {Ergen, Arzu and Iplık, E. Sinem and Ertugrul, Baris and Atas, Merve Nur and Kasarcı, Goksu and Cakmakoglu, Bedia} }
APA Ergen, A , Iplık, E , Ertugrul, B , Atas, M , Kasarcı, G , Cakmakoglu, B . (2020). Examination of the apoptotic effects of betulinic acid on renal cancer cell lines . Marmara Medical Journal , 33 (3) , 113-118 . DOI: 10.5472/marumj.815542
MLA Ergen, A , Iplık, E , Ertugrul, B , Atas, M , Kasarcı, G , Cakmakoglu, B . "Examination of the apoptotic effects of betulinic acid on renal cancer cell lines" . Marmara Medical Journal 33 (2020 ): 113-118 <https://dergipark.org.tr/en/pub/marumj/issue/57487/815542>
Chicago Ergen, A , Iplık, E , Ertugrul, B , Atas, M , Kasarcı, G , Cakmakoglu, B . "Examination of the apoptotic effects of betulinic acid on renal cancer cell lines". Marmara Medical Journal 33 (2020 ): 113-118
RIS TY - JOUR T1 - Examination of the apoptotic effects of betulinic acid on renal cancer cell lines AU - Arzu Ergen , E. Sinem Iplık , Baris Ertugrul , Merve Nur Atas , Goksu Kasarcı , Bedia Cakmakoglu Y1 - 2020 PY - 2020 N1 - doi: 10.5472/marumj.815542 DO - 10.5472/marumj.815542 T2 - Marmara Medical Journal JF - Journal JO - JOR SP - 113 EP - 118 VL - 33 IS - 3 SN - 1019-1941-1309-9469 M3 - doi: 10.5472/marumj.815542 UR - https://doi.org/10.5472/marumj.815542 Y2 - 2020 ER -
EndNote %0 Marmara Medical Journal Examination of the apoptotic effects of betulinic acid on renal cancer cell lines %A Arzu Ergen , E. Sinem Iplık , Baris Ertugrul , Merve Nur Atas , Goksu Kasarcı , Bedia Cakmakoglu %T Examination of the apoptotic effects of betulinic acid on renal cancer cell lines %D 2020 %J Marmara Medical Journal %P 1019-1941-1309-9469 %V 33 %N 3 %R doi: 10.5472/marumj.815542 %U 10.5472/marumj.815542
ISNAD Ergen, Arzu , Iplık, E. Sinem , Ertugrul, Baris , Atas, Merve Nur , Kasarcı, Goksu , Cakmakoglu, Bedia . "Examination of the apoptotic effects of betulinic acid on renal cancer cell lines". Marmara Medical Journal 33 / 3 (October 2020): 113-118 . https://doi.org/10.5472/marumj.815542
AMA Ergen A , Iplık E , Ertugrul B , Atas M , Kasarcı G , Cakmakoglu B . Examination of the apoptotic effects of betulinic acid on renal cancer cell lines. Marmara Med J. 2020; 33(3): 113-118.
Vancouver Ergen A , Iplık E , Ertugrul B , Atas M , Kasarcı G , Cakmakoglu B . Examination of the apoptotic effects of betulinic acid on renal cancer cell lines. Marmara Medical Journal. 2020; 33(3): 113-118.
IEEE A. Ergen , E. Iplık , B. Ertugrul , M. Atas , G. Kasarcı and B. Cakmakoglu , "Examination of the apoptotic effects of betulinic acid on renal cancer cell lines", Marmara Medical Journal, vol. 33, no. 3, pp. 113-118, Oct. 2020, doi:10.5472/marumj.815542