Effect of different immobilization media on breakdown of whey proteins by Streptococcus thermophilus

Year 2022, Volume: 35 Issue: 2, 139 - 142, 30.05.2022

Fatıma Zehra Safak Gokhan Bıcım Ayse Mine Yılmaz Burak Aksu A. Suha Yalcın

https://doi.org/10.5472/marumj.1120587

Abstract

Objective: In this study, we aimed to compare the efficiency of different immobilization media to facilitate breakdown of whey
proteins by Streptococcus thermophilus (S. thermophilus).

Materials and Methods: S. thermophilus was isolated from yoghurt. High-protein whey powder was present in fermentation media
and two-phase dispersion technique was used for immobilization of S. thermophilus in agar, agarose and κ-carrageenan. Total protein
after fermentation of whey proteins with S. thermophilus in different media was measured. We have also performed sodium dodecyl
sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis to observe changes in individual whey proteins after fermentation
in different media.

Results: Total protein concentration showed a significant decrease at the end of 24 hours of fermentation in all media. SDS-PAGE
results showed that the amount of both α-lactalbumin and β-lactoglobulin were reduced in all immobilization media compared to
control. The effect of κ-carrageenan was considerably higher compared to other media.

Conclusion: Our results showed that immobilization in κ-carrageenan increased the breakdown of whey proteins by S. thermophilus
and can be used to increase fermentation efficiency.

Keywords

α-lactalbumin, β-lactoglobulin, Fermentation, Immobilization, κ-carrageenan

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