Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells

Year 2023, Volume: 36 Issue: 1, 39 - 45, 31.01.2023

F. Tutku Aksoy Ayse Mine Yılmaz Gokhan Bıcım A. Suha Yalcın

https://doi.org/10.5472/marumj.1244676

Abstract

Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined
their effects on MCF-7 cells.
Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil (HO) and olive oil (OO) at a
protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied
to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species
(ROS) production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21(Waf1/Cip1) expressions
were investigated.
Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied
between 5-10% and no statistically significant effect was observed. There was no statistically significant difference in ROS production
and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased
cell migration compared to the control group. G2/M phase was significantly suppressed in WP:OO group compared to the control
group. WP:OO also increased the expression of p21(Waf1/Cip1) significantly when compared with the control group.
Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer
treatment.

Keywords

Whey proteins, Oleic acid, Cell survival, Apoptosis, Cell cycle

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