Effect of methylglyoxal on Parkinson’s disease pathophysiology in the rotenone model

Year 2024, Volume: 37 Issue: 2, 166 - 177, 31.05.2024

Yekta Çulpan Lara Ozden Yakup Gozderesi Beril Kocak Zeynep Hazal Baltaci Ayberk Denizli Betul Yılmaz Rezzan Gülhan

https://doi.org/10.5472/marumj.1480086

Abstract

Objective: Type 2 diabetes mellitus patients have been reported to have a higher incidence of Parkinson’s disease. This study aimed to
explore the effect of advanced glycation end products precursor methylglyoxal (MGO) on the pathophysiology of Parkinson’s disease
in a rotenone model.
Materials and Methods: Adult female Wistar rats (n=42) were divided into four groups. Rotenone toxicity was assessed by daily weight
measurements and mortality rates. Effect of MGO on blood glucose was evaluated. Locomotor activity, rearing, and rotarod tests
were performed to evaluate motor functions, and for neurodegeneration, tyrosine hydroxylase immunoreactivity in the striatum and
substantia nigra regions was assessed.
Results: The mortality rate was 9% in the rotenone-applied rats. The mean weight, locomotor activity, rearing activity, and longest time
spent on a rotarod were lower in the MGO+Rotenone group than in the Control group. Tyrosine hydroxylase immunoreactivity in the
striatum rostral to the anterior commissure in the MGO+Rotenone group was lower than that in the Control and MGO groups. The
number of tyrosine hydroxylase positive cells in the substantia nigra pars compacta was comparable among the groups.
Conclusion: When nigrostriatal degeneration was triggered, MGO was found to worsen motor dysfunction and increase damage to
dopaminergic neuron projections.

Keywords

Parkinson’s disease, Type 2 diabetes mellitus, Methylglyoxal, Rotenone, Tyrosine hydroxylase, Locomotor activity

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