Low-dose LPS and calorie restriction combined therapy for pancreatic ductal adenocarcinoma: an in vitro and in vivo study

Year 2025, Volume: 38 Issue: 1, 15 - 23, 29.01.2025

Bahar Beydogan , Başak Işıldar , Zeynep Mine Coşkun Yazıcı , Meral Koyutürk , Sema Bolkent

https://doi.org/10.5472/marumj.1627620

Abstract

Objective: This study aimed to evaluate the therapeutic effects of lipopolysaccharide (LPS) on Panc02 cells since LPS is an inflammatory
agent with the potential to activate the immune system and reorganize the tumor microenvironment. Moreover, calorie restriction
(CR) has been investigated in combination with LPS as a prospective adjuvant intervention for cancer therapy.
Materials and Methods: Panc02 cells were cultured in two distinct media with low and high-glucose concentrations, and cell viability
was investigated after applying varying doses of LPS to culture media. The in vivo effects of LPS and CR were investigated on the mice
subcutaneous pancreatic ductal adenocarcinoma (PDAC) tumor model in terms of tumor mass, histological examination, mRNA
expression profiles, and biochemical parameters.
Results: The lowest cell count was detected in the cells treated with 10 μg/ml LPS in low-glucose environments in vivo. Tumor mass
significantly decreased in the P+LPS+CR group in vivo. The mRNA expression analysis of tumor tissues indicated that P+LPS+CR acts
through NF-κB, JNK, and IL-6 signaling pathways.
Conclusion: Lipopolysaccharide alone is insufficient to show therapeutic effects, but it can inhibit tumor development by acting on
NF-κB and JNK pathways when combined with CR. This study gives insight into developing new treatment options for PDAC.

Keywords

Panc02, Lipopolysaccharide, Calorie restriction, Subcutaneous pancreatic ductal adenocarcinoma model, Cancer

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