Effect of 6-OHDA growth hormone on the calcium binding proteins located in the bulbus olfactorius in Parkinson model rats

Year 2025, Volume: 38 Issue: 1, 36 - 42, 29.01.2025

Özlem Kirazlı , Hatice Efekan Mehmet Acinikli Mesut Öztürk Serhat Demir Zeynep Büyükyılmaz , Ümit Süleyman Şehirli

Abstract

Objective: Parkinson’s disease is the second most common neurodegenerative disease. One of the earliest signs is hyposmia which
may appear in the premotor period resulting from the alpha-synucleinopathy that affects the neurons along the olfactory pathway.
Growth hormone (GH) stimulates cell growth and regeneration. The recovery of neuronal functions may be correlated with the GHs.
Calretinin can buffer calcium when intracellular calcium levels increase. This study aims to investigate the effect of GH on calretininpositive
neurons in the olfactory bulb of Parkinsonian rats.
Materials and Methods: 6-hydroxydopamine (6-OHDA) was injected intracranially. GH (0.15 mg/kg/day) and saline were administered
subcutaneously for treatment and control groups respectively. Calretinin staining was performed on the sections and calretininpositive
neurons in the lateral olfactory tract were counted.
Results: Thickness of the lateral olfactory tract was lesser in the control group. The decrease in the thickness of the tract may be an
important sign of hyposmia. The calretinin-positive neurons in the olfactory bulb of the treatment group are more in number when
compared with the control group.
Conclusion: The decrease in expression of calcium-binding proteins is closely related to neurodegenerative diseases. As a result, the
slight increase compared to the control group may be due to the neuroprotective effect of GH.

Keywords

Parkinson’s disease, Growth hormone, Calretinin, Lateral olfactory tract

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