In vitro evaluation of antioxidant and antimicrobial activities of some Centaurea L. species

Abstract

ABSTRACT: The purpose of this study was to investigate the antioxidant, antimicrobial activities
and total phenolic contents of ten methanol extracts obtained by maceration from
capitulums and aerial parts (except for capitulum) of five Centaurea species (C.stenolepis,
C.kilaea, C.cuneifolia, C.iberica, C.solstitialis subsp. solstitialis). Free radical scavenging
activities and total phenolic contents of the species were assayed by DPPH method and
Folin–Ciocalteu method, respectively. The antimicrobial activity of the extracts were tested
by the micro-broth dilution method against seven microbial species. In the DPPH radical
scavenging assay, the IC50 values of tested ten methanol extracts were in range 1.767-4.665
mg while the total phenol contents of four extracts were found to range between 4.825 and
12.460 mg GAE/g dry material. Four extract showed moderate activity against Pseudomonas
aeruginosa (MIC: 312 μg/ml), while six out of ten extracts exhibited moderate activity against
Candida albicans (MIC: 312 μg/ml). The methanol extract prepared from the aerial parts of
C.cuneifolia possessed weak activity against Staphylococcus aureus (MIC: 625 μg/ml).
KEYWORDS: Centaurea species, antioxidant activity, antimicrobial activity

Keywords

• Centaurea species, antioxidant activity, antimicrobial activity

In vitro evaluation of antioxidant and antimicrobial activities of some Centaurea L. species

Abstract

Bu çalışmanın amacı, 5 Centaurea türünün (C.stenolepis, C.kilaea, C.cuneifolia, C.iberica, C.solstitialis subsp. solstitialis) kapitulum ve kapitulumsuz toprak üstü kısımlarından maserasyonla hazırlanan toplam 10 metanol ekstresinin antioksidan, antimikrobiyal aktivitelerini ve toplam fenol içeriklerini araştırmaktır. Türlerin serbest radikal süpürücü aktivite tayinleri DPPH metodu ile, toplam fenolik madde miktar tayini ise Folin–Ciocalteu metoduyla yapıldı. Ekstrelerin antimikrobiyal aktivitesi 7 mikroorganizma türüne karşı mikro dilüsyon yöntemiyle test edildi. DPPH radikal süpürücü aktivite tayini deneyinde test edilen 10 ekstrenin İK50 değerlerinin 1.767-4.665 mg, toplam fenolik madde miktarlarının ise g kuru materyalde gallik asite eşdeğer olarak 4.825-12.460 mg aralığında değişkenlik gösterdiği görüldü. Dört ekstre Pseudomonas aeruginosa (MİK: 312 μg/ml)’ya, 7 ekstre Candida albicans (MİK: 312 μg/ml)’a karşı orta derecede, C.cuneifolia’nın toprak üstü kısımlarımlarından hazırlanan metanol ekstresi ise Staphylococcus aureus’a (MİK: 625 μg/ml) karşı zayıf bir antimikrobiyal aktivite göstermiştir

Keywords

• ANAHTAR SÖZCÜKLER: Centaurea türleri, antioksidan aktivite, antimikrobiyal aktivite

References

1. tapevi, İstanbul. 1999. 8.
2. Gao X, Ohlander M, Jeppsson N, Björk L, Trajkovski V.
3. Changes in Antioxidant Effects and Their Relationship
4. to Phytonutrients in Fruits of Sea Buckthorn (Hippophae
5. rhamnoides L.) during Maturation. J Agric Food Chem 2000; 48: 1485-90. 9.
6. Clinical and Laboratory Standards Institute (CLSI). Ref
7. erence Method for Broth Dilution Antifungal Susceptbil
8. ity Testing of Yeasts; Approved Standart M27-A2. CLSI,

9. Wayne, PA, USA. 2002.
10. Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically - Seventh Edition: Approved Standard M7-A7. CLSI, Wayne, PA, USA. 2006.