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Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb.

Year 2016, Volume: 20 Issue: 2, 111 - 115, 28.01.2016

Abstract

The essential oil obtained from the leaves and stems of Piper arborescens by hydrodistillation was analyzed by GC and GC–MS. Fifty components have been identified from the leaves and stems, comprise of thirty six (97.5%) and forty six (90.5%) components, respectively. The most abundant components in the leaves oil were β-phellandrene (24.3%), sabinene (16.3%), α-pinene (10.4%) and terpinen-4-ol (7.2%), while β-phellandrene (20.4%), methyl eugenol (11.0%) and β-caryophyllene (9.0%) were the main components in the stems oil. The essential oils were tested for antimicrobial activity by using disc diffusion and micro dilution methods for the minimum inhibitory concentration (MIC). The results showed that the leaves oil exhibited significant antimicrobial activity towards Staphylococcus aureus with MIC value 250 µg/mL, while stems oil showed the activity against Gram positive bacteria, Pseudomonas aeruginosa and fungal, Aspergillus niger each with MIC value 500 µg/mL.

References

  • Parmar VS, Jain SC, Bisht KS, Jain R, Taneja P, Jha A, Tyagi OD, Prasad AK, Wengel J, Olsen CE, Boll PM. Phytochemistry of the genus Piper. Phytochemistry 1997; 46: 597-673.
  • Martins AP, Salguero L, Vila R, Tomi F, Canigueral S, Casanova J, Proenca da Cunha A, Adzet T. Essential oils from Piper species. Phytochemistry 1998; 49: 2019-2023.
  • Santos PRD, Moreira DL, Guimaraes EF, Kaplan MAC. Essential oil analysis of 10 Piperaceae species from the Brazilian Atlantic forest. Phytochemistry 2001; 58: 547-551.
  • Costantin MB, Sartorelli P, Limberger R, Henriques AT, Steppe M, Ferreira MJP, Ohara MT, Emerenciano VP, Kato MJ. Essential oils from Piper cernuum and Piper regnellii: Antimicrobial activities and analysis by GC/MS and 13C-NMR. Planta Med 2001; 67: 771-773.
  • Navickiene HMD, Morandim AA, Alecio AC, Regasini LO, Bergamo DCB, Telascrea M, Cavalheiro AJ, Lopes MN, Bolzani VS, Furlan M, Marques MOM, Young MCM, Kato MJ. Composition and antifungal activity of essential oils from Piper aduncum, Piper arboreum and Piper tuberculatum. Quim Nova 2006; 29: 467-470.
  • Lin TT, Lu SY. Piperaceae in Flora of Taiwan, 2nd Edition, Editorial Committee of the Flora of Taiwan, Taipei, Taiwan, 1996.
  • Duh CY, Wu YC, Wang SK. Cytotoxic pyridone alkaloids from Piper aborescens. Phytochemistry 1990; 29: 2689-2691.
  • Lee FP, Chen YC, Chen JJ, Tsai IL, Chen IS. Cyclobutanoid Amides from Piper arborescens. Helvet Chim Acta 2004; 87: 463-468.
  • Tsai IL, Lee FP, Wu CC, Duh CY, Ishikawa T, Chen JJ, Chen YC, Seki H, Chen IS. New cytotoxic cyclobutanoid amides, a new furanoid lignin and anti-platelet aggregation constituents from Piper aborescens. Planta Med 2005; 71: 535-542.
  • Geran RI, Greenberg NH, Macdonald MM, Schumacher AM, Abbott BJ. Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemother Rep 1972; 7: 7-9.
  • Adams RP. Identification of essential oil by gas chromatography-quadrupole/mass spectroscopy, 4th Ed. Allured Publishing Corporation, Carol Stream IL, USA, 2001.
  • Salleh WMNHW, Ahmad F, Yen KH. Chemical compositions and antimicrobial activity of the essential oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae). Nat Prod Commun 2014; 9: 1795–1798.
  • Jirovetz L, Buchbauer G, Abraham GT, Shafi MP. Chemical composition and olfactory characterization of Acmella radicans (Jacq.) R.K. Jansen var. radicans from southern India. Flavour Frag J 2006; 21: 88-91.
  • Chieng TC, Assim ZB, Fasihuddin BA. The essential oil composition of the leaves of five Piper species for taxonomic purposes. ACGC Chem Res Commun 2005; 19: 6-12.
  • Marjo K, Eija P, James ES. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and mor-phological differences of genotypes. Biochem Syst Ecol 2001; 29: 267-285.
  • Filippi JJ, Lanfranchi DA, Prado S, Baldovini N, Meierhenrich UJ. Composition enantiomeric distribution and antibacterial activity of the essential oil of Achillea ligustica All from Corsica. J Agric Food Chem 2006; 54: 6308-6313.
  • Marzoug HNB, Romdhane M, Lebrihi A, Mathieu F, Couderc F, Abderraba M, Khouja ML, Bouajila J. Eucalyptus oleosa essential oils: Chemical composition and antimicrobial and antioxidant activities of the oils from different plant parts (Stems, Leaves, Flowers and Fruits). Molecules 2011; 16: 1695-1709.

Essential Oil Compositions and Antimicrobial Activity of Piper

Year 2016, Volume: 20 Issue: 2, 111 - 115, 28.01.2016

Abstract

Piper arboresanın’un yaprak ve saplarından hidrodistilasyonla elde edilen uçucu yağlar GC ve GC-MS ile analiz edildi. Yaprak ve sap uçucu yağlarında sırasıyla otuzaltı bileşik (% 97.5) ve kırkaltı bileşik (% 90.5) olmak üzere elli bileşik tanımlanmıştır.. Yaprak uçucu yağı içinde en bol bulunan bileşenler β-fellandren (% 24.3), sabinen (% 16.3), α-pinen (% 10.4) ve terpinen-4-ol (7.2%) iken sap uçucu yağında ise β-fellandren (% 20.4), metil

References

  • Parmar VS, Jain SC, Bisht KS, Jain R, Taneja P, Jha A, Tyagi OD, Prasad AK, Wengel J, Olsen CE, Boll PM. Phytochemistry of the genus Piper. Phytochemistry 1997; 46: 597-673.
  • Martins AP, Salguero L, Vila R, Tomi F, Canigueral S, Casanova J, Proenca da Cunha A, Adzet T. Essential oils from Piper species. Phytochemistry 1998; 49: 2019-2023.
  • Santos PRD, Moreira DL, Guimaraes EF, Kaplan MAC. Essential oil analysis of 10 Piperaceae species from the Brazilian Atlantic forest. Phytochemistry 2001; 58: 547-551.
  • Costantin MB, Sartorelli P, Limberger R, Henriques AT, Steppe M, Ferreira MJP, Ohara MT, Emerenciano VP, Kato MJ. Essential oils from Piper cernuum and Piper regnellii: Antimicrobial activities and analysis by GC/MS and 13C-NMR. Planta Med 2001; 67: 771-773.
  • Navickiene HMD, Morandim AA, Alecio AC, Regasini LO, Bergamo DCB, Telascrea M, Cavalheiro AJ, Lopes MN, Bolzani VS, Furlan M, Marques MOM, Young MCM, Kato MJ. Composition and antifungal activity of essential oils from Piper aduncum, Piper arboreum and Piper tuberculatum. Quim Nova 2006; 29: 467-470.
  • Lin TT, Lu SY. Piperaceae in Flora of Taiwan, 2nd Edition, Editorial Committee of the Flora of Taiwan, Taipei, Taiwan, 1996.
  • Duh CY, Wu YC, Wang SK. Cytotoxic pyridone alkaloids from Piper aborescens. Phytochemistry 1990; 29: 2689-2691.
  • Lee FP, Chen YC, Chen JJ, Tsai IL, Chen IS. Cyclobutanoid Amides from Piper arborescens. Helvet Chim Acta 2004; 87: 463-468.
  • Tsai IL, Lee FP, Wu CC, Duh CY, Ishikawa T, Chen JJ, Chen YC, Seki H, Chen IS. New cytotoxic cyclobutanoid amides, a new furanoid lignin and anti-platelet aggregation constituents from Piper aborescens. Planta Med 2005; 71: 535-542.
  • Geran RI, Greenberg NH, Macdonald MM, Schumacher AM, Abbott BJ. Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemother Rep 1972; 7: 7-9.
  • Adams RP. Identification of essential oil by gas chromatography-quadrupole/mass spectroscopy, 4th Ed. Allured Publishing Corporation, Carol Stream IL, USA, 2001.
  • Salleh WMNHW, Ahmad F, Yen KH. Chemical compositions and antimicrobial activity of the essential oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae). Nat Prod Commun 2014; 9: 1795–1798.
  • Jirovetz L, Buchbauer G, Abraham GT, Shafi MP. Chemical composition and olfactory characterization of Acmella radicans (Jacq.) R.K. Jansen var. radicans from southern India. Flavour Frag J 2006; 21: 88-91.
  • Chieng TC, Assim ZB, Fasihuddin BA. The essential oil composition of the leaves of five Piper species for taxonomic purposes. ACGC Chem Res Commun 2005; 19: 6-12.
  • Marjo K, Eija P, James ES. Variation in volatile compounds from tansy (Tanacetum vulgare L.) related to genetic and mor-phological differences of genotypes. Biochem Syst Ecol 2001; 29: 267-285.
  • Filippi JJ, Lanfranchi DA, Prado S, Baldovini N, Meierhenrich UJ. Composition enantiomeric distribution and antibacterial activity of the essential oil of Achillea ligustica All from Corsica. J Agric Food Chem 2006; 54: 6308-6313.
  • Marzoug HNB, Romdhane M, Lebrihi A, Mathieu F, Couderc F, Abderraba M, Khouja ML, Bouajila J. Eucalyptus oleosa essential oils: Chemical composition and antimicrobial and antioxidant activities of the oils from different plant parts (Stems, Leaves, Flowers and Fruits). Molecules 2011; 16: 1695-1709.
There are 17 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Wan Mohd Nuzul Hakimi Wan Salleh

Farediah Ahmad

Heng Yen Khong This is me

Publication Date January 28, 2016
Published in Issue Year 2016 Volume: 20 Issue: 2

Cite

APA Salleh, W. M. N. H. W., Ahmad, F., & Khong, H. Y. (2016). Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb. Marmara Pharmaceutical Journal, 20(2), 111-115. https://doi.org/10.12991/mpj.20162071871
AMA Salleh WMNHW, Ahmad F, Khong HY. Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb. Marmara Pharm J. February 2016;20(2):111-115. doi:10.12991/mpj.20162071871
Chicago Salleh, Wan Mohd Nuzul Hakimi Wan, Farediah Ahmad, and Heng Yen Khong. “Essential Oil Compositions and Antimicrobial Activity of Piper Arborescens Roxb”. Marmara Pharmaceutical Journal 20, no. 2 (February 2016): 111-15. https://doi.org/10.12991/mpj.20162071871.
EndNote Salleh WMNHW, Ahmad F, Khong HY (February 1, 2016) Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb. Marmara Pharmaceutical Journal 20 2 111–115.
IEEE W. M. N. H. W. Salleh, F. Ahmad, and H. Y. Khong, “Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb”., Marmara Pharm J, vol. 20, no. 2, pp. 111–115, 2016, doi: 10.12991/mpj.20162071871.
ISNAD Salleh, Wan Mohd Nuzul Hakimi Wan et al. “Essential Oil Compositions and Antimicrobial Activity of Piper Arborescens Roxb”. Marmara Pharmaceutical Journal 20/2 (February 2016), 111-115. https://doi.org/10.12991/mpj.20162071871.
JAMA Salleh WMNHW, Ahmad F, Khong HY. Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb. Marmara Pharm J. 2016;20:111–115.
MLA Salleh, Wan Mohd Nuzul Hakimi Wan et al. “Essential Oil Compositions and Antimicrobial Activity of Piper Arborescens Roxb”. Marmara Pharmaceutical Journal, vol. 20, no. 2, 2016, pp. 111-5, doi:10.12991/mpj.20162071871.
Vancouver Salleh WMNHW, Ahmad F, Khong HY. Essential Oil Compositions and Antimicrobial Activity of Piper arborescens Roxb. Marmara Pharm J. 2016;20(2):111-5.