Research Article
Year 2016,
Volume: 20 Issue: 2, 184 - 190, 27.02.2016
Abstract
References
- Ghemawat MS. Polychromatic staining with toluidine blue O for studying the host-parasite relationships in wheat leaves of Erysiphe graminis f. sp. tritici. Physiol Plant Pathol 1977; 11: 251–3.
- Heath MC. Light and electron microscope studies of the interactions of host and non-host plants with cowpea rust— Uromyces phaseoli var. vignae. Physiol Plant Pathol 1974; 4: 403-14.
- Shipton WA, Brown JF. A whole-leaf clearing and staining technique to demonstrate hostpathogen relationships of wheat stem rust. Phytopathology 1962; 52: 1313.
- Saha DC, Jackson MA, Johnson-Cicalese JM. A rapid staining method for detection of Endophytic fungi in turf and forage grasses. Phytopathology 1988; 78: 237-9.
- McBride MC. A method of demonstrating rust hyphae and haustoria in unsectioned leaf tissue. Am J Bot 1936; 23: 686–8.
- Bandoni RJ. Safranin O as a rapid nuclear stain for fungi. Mycologia 1979; 11: 873–4.
- Trese AT, Loschke DC. High contrast resolution of mycelia of pathogenic fungi in corn tissue after staining with calcofluor and destaining with cellulose. Phytopatology 1990; 80: 196– 200.
- Algan G. Bitkisel Dokular İçin Mikroteknik. Fırat Üni Fen Ed Fak Yay Bot No:1. İstanbul. 1981.
- Johansen DA. Plant microtechnique. McGraw-Hill Book Co., Inc., New York and London. 1940, pp.553.
- Yeung ECT, Stasolla C, Sumner MJ, Huang BQ. Plant Microtechniques and Protocols, Springer International Publishing (EBook). Switzerland. 2015.
- Baytop A. Bitkisel Drogların Anatomik Yapısı. İstanbul Üni Yay 6. Baskı No: 32. İstanbul. 1981.
- Sass J. Botanical Microtechnique. Third Edition. Iowa State University Press, Iowa. 1958.
- Popham RA, Johnson TJ, Chan AP. Safranin and anilin blue with Delafield’s hematoxylin for staining cell walls in shoot apexes. Stain Tech 1948; 23:185-90.
- Balatinecz JJ, Kennedy RW. Maturation of ray parenchyma cells in pine. Forest Prod J 1967; 17: 57-64.
- Jensen WA. Botanical Histochemistry: Principles and Practice. Freeman, San Francisco. 1962.
Year 2016,
Volume: 20 Issue: 2, 184 - 190, 27.02.2016
Abstract
Bu çalışmada alkol ile fikse edilmiş bitki örneklerinin el kesiti ile preparat yapımında, dokuların net bir şekilde ayırt edilebilmesini sağlayan yeni bir ikili boyama metodu geliştirildi. Bu yeni metot, el kesitlerinde kullanılabilmesi ve renk farkıyla dokuları net bir şekilde ayırt etmesi bakımından pratiklik ve kesinlik sağlamaktadır. Bu metotta, safranin ve fast-green boyalarının belirli oranlarda karışımından oluşan ikili boya, hem monokotil hem de dikotil bitki örneklerinde kullanılabilmekte ve uzun süre oda sıcaklığında bozulmadan saklanabilmektedir. Bu makalede, bu metodun uygulandığı çeşitli bitki örneklerine ait anatomik kesitlerin fotoğrafları çekilerek, çeşitli dokular gösterilmiştir.
Keywords
References
- Ghemawat MS. Polychromatic staining with toluidine blue O for studying the host-parasite relationships in wheat leaves of Erysiphe graminis f. sp. tritici. Physiol Plant Pathol 1977; 11: 251–3.
- Heath MC. Light and electron microscope studies of the interactions of host and non-host plants with cowpea rust— Uromyces phaseoli var. vignae. Physiol Plant Pathol 1974; 4: 403-14.
- Shipton WA, Brown JF. A whole-leaf clearing and staining technique to demonstrate hostpathogen relationships of wheat stem rust. Phytopathology 1962; 52: 1313.
- Saha DC, Jackson MA, Johnson-Cicalese JM. A rapid staining method for detection of Endophytic fungi in turf and forage grasses. Phytopathology 1988; 78: 237-9.
- McBride MC. A method of demonstrating rust hyphae and haustoria in unsectioned leaf tissue. Am J Bot 1936; 23: 686–8.
- Bandoni RJ. Safranin O as a rapid nuclear stain for fungi. Mycologia 1979; 11: 873–4.
- Trese AT, Loschke DC. High contrast resolution of mycelia of pathogenic fungi in corn tissue after staining with calcofluor and destaining with cellulose. Phytopatology 1990; 80: 196– 200.
- Algan G. Bitkisel Dokular İçin Mikroteknik. Fırat Üni Fen Ed Fak Yay Bot No:1. İstanbul. 1981.
- Johansen DA. Plant microtechnique. McGraw-Hill Book Co., Inc., New York and London. 1940, pp.553.
- Yeung ECT, Stasolla C, Sumner MJ, Huang BQ. Plant Microtechniques and Protocols, Springer International Publishing (EBook). Switzerland. 2015.
- Baytop A. Bitkisel Drogların Anatomik Yapısı. İstanbul Üni Yay 6. Baskı No: 32. İstanbul. 1981.
- Sass J. Botanical Microtechnique. Third Edition. Iowa State University Press, Iowa. 1958.
- Popham RA, Johnson TJ, Chan AP. Safranin and anilin blue with Delafield’s hematoxylin for staining cell walls in shoot apexes. Stain Tech 1948; 23:185-90.
- Balatinecz JJ, Kennedy RW. Maturation of ray parenchyma cells in pine. Forest Prod J 1967; 17: 57-64.
- Jensen WA. Botanical Histochemistry: Principles and Practice. Freeman, San Francisco. 1962.
There are 15 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Article |
| Authors | |
| Publication Date | February 27, 2016 |
| Published in Issue | Year 2016 Volume: 20 Issue: 2 |
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