Edaravone Ameliorates Valproate-Induced Gingival Toxicity by Reducing Oxidative-Stress, Inflammation and Tissue Damage

Year 2016, Volume: 20 Issue: 3, 243 - 251, 14.03.2016

Şehkar Oktay , Burcin Alev Leyla Koc Ozturk Sevim Tunali Sezin Demirel Ebru Emekli Alturfan , Tugba Tunali-akbay , Serap Akyuz Refiye Yanardag , Aysen Yarat

Abstract

Valproic acid (2-n-propylpentanoic acid, VPA),the most widely used antiepileptic drug, has potential adverse effects and it can disrupt the oxidant and antioxidant balance. Edaravone (3-methyl-1-phenyl-2-pyrazoline-5-one, EDA) is a potent free radical scavenger. In this study, the effect of EDA on gingiva in VPA induced toxicity was investigated. Female Sprague Dawley rats were randomly divided into four groups: control group, EDA (30 mg/kg/day) given group, VPA (0.5 g/kg/day) given group,  and VPA+EDA (in same dose and time) given group. EDA and VPA were given intraperitoneally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and reduced glutathione (GSH) levels and catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), myeloperoxidase (MPO), alkaline phosphatase (ALP), acid phosphatase (ACP), sodium potassium ATPase (Na⁺/K⁺-ATPase) and tissue factor (TF) activities were determined in gingiva homogenates. The VPA-induced increases were statistically significant for MPO (p<0.01), ACP (p<0.01), Na⁺/K⁺-ATPase (p<0.05) and TF (p<0.01) activities, but not for LPO level and ALP activities.  EDA treatment markedly blunted all such elevated anomalies. Conclusively: VPA induced oxidative- and inflammatory-gingival tissue damage, reactions that were appreciably reversed by concurrent administration of EDA.

 

Key words: Gingiva, valproic acid, edaravone,  oxidative stress, inflammation

Keywords

Gingiva , valproic acid , edaravone , oxidative stress , inflammation