Antibacterial, cytotoxicity, and phytotoxicity profiles of three medicinal plants collected from Pakistan

Abstract

The purpose of present research was to examine the antibacterial,

cytotoxic, and phytotoxic profiles of three important Pakistani

medicinal plants viz., Teucrium stocksianum, Chenopodium

botrys, and Micromeria biflora. The antibacterial, cytotoxicity,

and phytotoxicity activities of samples extracted from these

plants were evaluated by a modified agar well-diffusion method,

brine shrimps cytotoxic assay, and Lemna acquinoctialisbased

phytotoxic assay, respectively. Results revealed marked

susceptibility of both the crude extracts and the aqueous

fractions of these plants against K. pneumonia and B. subtilis.

Methanolic extracts and aqueous fractions exhibited significant

cytotoxicity in a concentration-dependent manner. Similarly,

an outstanding phytotoxic effect was observed for the extracts/

fractions. Accordingly, the extracts and fractions of the

aforementioned plants possess potential antibacterial, cytotoxic,

and phytotoxic effects which could be useful in the search and

development of new pharmaceutical agents.

Keywords

• Teucrium stocksianum,Chenopodium botrys,Micromeria biflora,antibacterial activity,cytotoxicity

Pakistan’dan toplanan üç tıbbi bitkinin antibakteriyel etkileri, sitotoksisite ve fitotoksisite profilleri

Abstract

Mevcut araştırmanın amacı, Pakistan’da üç önemli tıbbi

bitki olan Teucrium stocksianum, Chenopodium botrys, ve

Micromeria biflora bitkilerinin antibakteriyel, sitotoksik ve

fitotoksik profilini incelemektir. Örneklerin antibakteriyel,

sitotoksik ve fitotoksik aktiviteleri sırasıyla modifiye agar

difüzyon yöntemi, Brine shrimp sitotoksik yöntemi ve Lemna

acquinoctialis’e dayanan fitotoksik yöntemi kullanılarak

değerlendirildi. Sonuçlar bu bitkilerin hem özütlerinin hemde

sulu fraksiyonlarının K. pneumonia ve B. subtilis suşlarına karşı

belirgin şekilde duyarlı olduğunu gösterdi. Metanol özütleri ve

sulu fraksiyonlar konsantrasyona bağlı olarak önemli sitotoksik

aktivite gösterdiler. Benzer olarak, bu özüt ve fraksiyonların

önemli derecede fitotoksik etki gösterdikleri gözlendi. Bu

sonuçlara göre, özütler/fraksiyonlar yeni farmasötik ajanların

gelişmesi için kullanılabilecek güçlü antibakteriyel, sitotoksik ve

fitotoksik aktiviteye sahiptir.

Keywords

• Teucrium stocksianum,Chenopodium botrys,Micromeria biflora,antibakteriyel aktivite,sitotoksisite ve fitotoksisite

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