Chemical Profile of Malva Neglecta and Malvella Sherardiana by Lc- MS/MS, GC/MS and Their Anticholinesterase, Antimicrobial and Antioxidant Properties With Aflatoxin-Contents

Abstract

The purpose of the present work was to calculate the phenolic

and the fatty acids profiles by LC-MS/MS and GC/MS with their

antioxidant, anticholinesterase and antimicrobial activities, and

aflatoxin contents of Malva neglecta and Malvella sherardiana.

The phenolic contents of M. neglecta and M. sherardiana were

determined by LC-MS/MS. Malic and 4-OH benzoic acids were

found to be the most abundant compounds in M. neglecta and M.

sherardiana, respectively. On the other hand, essential oils and

fatty acids composition were determined by GC/MS analysis.

The methanol extracts of the plants showed the highest effect

in all antioxidant assays of this study. The methanol extract of

M. neglecta showed the highest activity among the petroleum

ether, acetone, methanol and water extracts against acetyl- and

butyryl- cholinesterase enzymes (53.68% and 63.95% inhibition

ratio, respectively). The acetone extracts of M. neglacta and M.

sherardiana exhibited moderate activity against C. albicans with

18 and 17 mm inhibion zone diameter, respectively. The results

of the present study is also showed that the M. neglecta and M.

sherardiana can also be used as a food source due to its high

phenolic acid content and good antioxidant property. Also, the

samples were aflatoxin free.

Keywords

• Anticholinesterase; antioxidant; Malva neglecta; Malvella sherardiana; LC-MS/MS,GC-MS

Chemical Profile of Malva Neglecta and Malvella Sherardiana by Lc- MS/MS, GC/MS and Their Anticholinesterase, Antimicrobial and Antioxidant Properties With Aflatoxin-Contents

Abstract

Bu çalışmanın amacı Malva neglecta ve Malvella sherardiana

türlerinin antioksidan, antikolinesteraz ve antimikrobiyal

aktiviterini, aflatoksin içeriği ile fenolik bileşik ve yağ asidi

profillerini LC-MS/MS ve GC/MS ile belirlemektir. İncelenen

yirmi yedi bileşikten, M. neglecta ve M. sherardiana türlerinde en

bol bulunan bileşiklerin sırasıyla malik (13108.97 μg/g) ve 4-OH

benzoik asit (2200.57 μg/g) olduğu saptanmıştır. M. neglecta

türünün metanol ekstresindeki toplam flavonoid içeriğin (15.58

μg QEs/mg ekstre) toplam fenolik içerikten (68.29 μg PEs/

mg ekstre) daha az olduğu belirlenmiştir. Bitkilerin metanol

ekstresi çalışılan antioksidan aktivite belirleme yöntemlerinin

tümünde en yüksek aktiviteyi göstermiştir. En iyi kolinesteraz

enzim inhibisyonu ise M. neglecta türünün metanol ekstresi

tarafından kaydedilmiştir (%53.68 AChE enzim inhibisyonu ve

%63.95 BChE enzim inhibisyonu). Bitkilerin aseton ekstreleri

ise C. albicans türüne karşı orta düzeyde antimikrobiyal

aktivite göstermiştir. Bu çalışmadan elde edilen sonuçlar, M.

neglecta ve M. sherardiana türlerinin yüksek fenolik asit içeriği

ve antioksidan aktivitesinden dolayı besin kaynağı olarak

kullanılabileceğini göstermektedir. Ayrıca ekstreler aflatoxin

içermemektedir.

Keywords

• Antikolinesteraz; antioxidan; Malva neglecta; Malvella sherardiana; LC-MS/MS,GC-MS

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