Cytotoxicity, Radical Scavenging, Antioxidant Properties and Chemical Composition of the Essential Oil of Satureja cilicica P.H. Davis from Turkey

Abstract

Satureja cilicica P.H. Davis is an endemic species of Lamiaceae,

distributed in Turkey. In this study, the essential oil of S.

cilicica was obtained by hydrodistillation from the aerial

parts. The essential oil was analyzed by GC/MS and the main

constituents are identified. Radical scavenging capacities of oil

obtained from S. cilicica were determined by using DPPH and

ABTS radical scavenging assays. In addition, ferric-reducing

power of the essential oil was detected. Cytotoxic activity

of the essential oil was determined by MTT assay after 24 h

treatment of MCF-7. The major components of the essential

oil are p-cymene (17.68%), carvacrol (14.02%), γ-terpinene

(11.23 %) and thymol (8.76%). Radical scavenging capacities of

the essential oil were measured as 3.28±0.02 and 238.15±3.59

mg Trolox equivalent per milliliter of oil in term of DPPH and

ABTS radical scavenging, respectively. Ferric-reducing power

of essential oil was found 39.76±3.66 mg Trolox equivalent per

milliliter of essential oil. Growth inhibition effect of essential

oil against MCF-7 cancer cell is established. According to the

results, essential oil of S. cilicica exhibit low cytotoxic activity,

with IC50 values, with IC50 value of 268 μg/mL. The findings of

the analyzed species are compared with the results of previous

studies.

Keywords

• ABTS,cytotoxicity,DPPH,essential oil composition,thyme

Türkiye’de Yetişen Satureja cilicica P.H. Davis Türünün Uçucu Yağının Sitotoksisitesi, Antioksidan Aktivitesi ve Kimyasal Kompozisyonu

Abstract

Satureja cilicica P.H. Davis Lamiaceae familyasının Türkiye’de

yetişen endemik bir türüdür. Bu çalışmada, S. cilicica’nın uçucu

yağı bitkinin toprak üstü kısımlarından su distilasyonu yöntemi

ile elde edilmiştir. Uçucu yağ GC/MS ile analiz edilmiş ve ana

bileşenleri belirlenmiştir. S. cilicica’dan elde edilen yağın radikal

süpürme kapasiteleri DPPH radikali ve ABTS radikali süpürme

yöntemi ile belirlenmiştir. Ayrıca uçucu yağın ferrik indirgeme

gücü tespit edilmiştir. Uçucu yağın sitotoksik aktivitesi 24

saat MCF-7 ile muamele edildikten sonra MTT yöntemi

ile belirlenmiştir. p-Simen (%17.68), karvakrol %(14.02),

γ-terpinen (%11.23 ) ve timol (%8.76) uçucu yağın ana

bileşenleri olarak saptanmıştır. Uçucu yağın radikal süpürme

kapasiteleri sırasıyla DPPH ve ABTS radikal süpürme yöntemi

ile 3,28±0,02 ve 238,15±3,59 mg eşdeğer trolox/mL uçucu yağ

olarak ölçülmüştür. Uçucu yağın ferrik indirgeme gücü, 39,76

± 3,66 mg eşdeğer trolox/mL uçucu yağ olarak bulunmuştur.

Uçucu yağın MCF-7 kanser hücresine karşı büyüme inhibisyon

etkisi araştırılmıştır. Sonuç olarak, S. cilicica’nın uçucu yağının

268 μg/mL IC50 değeri ile düşük sitotoksik aktiviteye sahip

olduğu görülmüştür. Analiz edilen türden elde edilen veriler

önceki çalışmalar ile karşılaştırılmıştır.

Keywords

• ABTS,DPPH,kekik,sitotoksisite

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