Toplam fenolik içerik ve antioksidan kapasite tayininde kullanılan başlıca spektrofotometrik yöntemler

Year 2013, Volume: 17 Issue: 2, 93 - 103, 24.10.2014

Ebru Büyüktuncel

Abstract

Bu makale, antioksidan aktivite ölçmek için kullanılan başlıca spektrofotometrik yöntemler
hakkında bilgi vermektedir. İdeal olarak, antioksidan aktivite hem in vitro hem de in
vivo yöntemlerle tayin edilmelidir. Fakat in vivo çalışmaların yüksek maliyeti nedeniyle çoğu
ürün in vitro yöntemlerle değerlendirilir. Reaksiyon mekanizmalarına göre antioksidan kapasite
tayinleri hidrojen transferine dayanan reaksiyonlar (HAT) ve tek elektron transferine
dayanan reaksiyonlar (SET) olmak üzere iki gruba ayrılır. HAT mekanizmasına dayanan başlıca
tayin yöntemleri oksijen radikal absorbsiyon kapasitesi yöntemi (ORAC), toplam radikal
tuzaklayıcı antioksidan parametre yöntemi (TRAP) ve karotenoid (krosin) ağartma yöntemidir.
SET mekanizmasına dayanan başlıca tayin yöntemleri Folin-Ciocalteu reaktifi (FCR) yöntemi,
Troloks (6-hidroksi-2,5,7,8-tetrametilkroman-2-karboksilik asit) eşdeğeri antioksidan
kapasite yöntemi (TEAC), demir (III) iyonu indirgeyici antioksidan güç yöntemi (FRAP),
2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürme kapasitesi yöntemi ve Cu(II)’nin oksidan
olarak kullanıldığı toplam antioksidan potansiyel yöntemi (CUPRAC)’dir.

Keywords

Antioksidan kapasite, toplam fenolik içerik, hidrojen atomu transfer reaksiyonu, elektron transfer reaksiyonu

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