Quality control evaluation and acetylcholinesterase inhibitory activity of Galanthus woronowii Losinsk.

Year 2011, Volume: 15 Issue: 2, 48 - 51, 07.03.2014

Ahmet Emir Nehir Ünver Somer Gülen Kaya , Buket Sarıkaya Mustafa Önür

Abstract

ABSTRACT: Aerial and underground parts of Galanthus woronowii Losinsk., a wild growing
species in north-eastern Anatolia, were collected during flowering period. Quality control
and acetylcholinesterase inhibitory activity determinations were carried out on Bulbus and
Herba Galanthi prepared from plants collected from two different localities. In the context of
quality control studies, contents of humidity, total ash, sulphated ash, acid-insoluble ash
and total alkaloids of the drug specimens were determined and found to range between
8.463-9.343 %, 6.950-14.947 %, 9.743-17.930 %, 1.102-3.565 % and 0.247-0.499 %, respectively.
Additionally, acetylcholinesterase inhibitory activity of the alkaloidal extracts prepared from
the drug specimens were determined by using Thin Layer Chromatography (TLC) combined
with a bioautographic assay based on in vitro Ellman method. All of the alkaloidal extracts
displayed acetylcholinesterase inhibitory activity.
KEY WORDS: Galanthus woronowii, Amaryllidaceae, Quality control determination, Acetylcholinesterase
inhibitory activity

Keywords

-

Quality control evaluation and acetylcholinesterase inhibitory activity of Galanthus woronowii Losinsk

Abstract

Kuzeydoğu Anadolu’da doğal olarak yetişen Galanthus woronowii Losinsk.’in toprak üstü ve toprak altı kısımları bitki çiçekli dönemde iken toplanmıştır. İki farklı lokaliteden toplanan bitkilerden hazırlanan Bulbus ve Herba Galanthi drogları üzerinde kalite kontrol ve asetilkolinesteraz inhibitör aktivite tayinleri gerçekleştirilmiştir. Kalite kontrol çalışmaları kapsamında drog örneklerinin nem, total kül, sülfat külü, hidroklorik asitte çözünmeyen kül ve total alkaloit içerikleri saptanmış ve bunların sırasıyla % 8.463-9.343, % 6.950-14.947, % 9.743-17.930, % 1.102-3.565 ve % 0.247-0.499 arasında değiştiği bulunmuştur. İlaveten, drog örneklerinden hazırlanan alkaloit ekstrelerinin asetilkolinesteraz inhibitör aktiviteleri in vitro Ellman yöntemine dayalı biyootografik bir deney ile kombine edilen İnce Tabaka Kromatografisinden (İTK) yararlanılarak tespit edilmiştir. Bütün alkaloit ekstreleri asetilkolinesteraz inhibitör aktivite göstermiştir

Keywords

ANAHTAR KELİMELER: Galanthus woronowii, Amaryllidaceae, Kalite kontrol tayini, Asetilkolinesteraz inhibitör aktivite

References

• Davis AP. Galanthus L. In: Flora of Turkey and the East Aegean Islands. Editors: Adil Güner, Neriman Özhatay, Tuna Ekim, Kemal Hüsnü Can Başer, Edinburgh University Press, Edinburgh. 2000, vol. 11, pp.265-270.
• Davis AP, Özhatay N. Galanthus trojanus: a new species of Galanthus (Amaryllidaceae) from north-western Turkey. Bot J Linn Soc. 2001; 137: 409-412.
• Davis AP. The genus Galanthus. In: A Botanical Magazine Monograph. Editor: Brian Mathew, Timber Press Inc., Oregon. 1999, pp. 165-170.
• Davis AP. The genus Galanthus-snowdrops in the wild. In: Snowdrops a Monograph of Cultivated Galanthus. Editors: Matt Bishop, Aaron Davis, John Grimshaw, Griffin Press Publishing Ltd., Cheltenham. 2006, pp. 44-47.
• Özhatay N. Diversity of bulbous monocots in Turkey with special reference. Chromosome numbers. Pure Appl Chem 2002; 74: 547-555.
• Tarım ve Köy İşleri Bakanlığı, Doğal Çiçek Soğanlarının 2010 Yılı İhracat Listesi Hakkında Tebliğ (2009/55), Resmi Gazete, Sayı: 27404.
• Proskurnina NF, Areshkina LY. On the alkaloids of Galanthus woronowii, J Gen Chem USSR 1947; 17: 1216- 1219.
• Proskurnina NF, Yakovleva AP. Alkaloids of Galanthus woronowii. V. Isolation of galanthamidine. J Gen Chem USSR 1956; 26: 179-180.
• Yakovleva AP. The Alkaloids of Galanthus woronowii. VII. Isolation of tazettine. J Gen Chem USSR 1963; 33: 1647-1648.
• Kintsurashvili L, Vachnadze V. Plants of the Amaryllidaceae family grown and introduced in Georgia: a source of galanthamine. Pharm Chem J-USSR 2007; 41: 492-494.
• Szlàvik L, Gyuris À, Minàrovits J, Forgo P, Molnàr J, Hohmann J. Alkaloids from Leucojum vernum and antiretroviral activity of Amaryllidaceae alkaloids. Planta Med 2004; 70: 871-873.
• Lamoral-Theys D, Andolfi, A, Goietsenoven, GV, Cimmino A, Le Calve B, Wauthoz N, Megalizzi V, Gras T, Bruyere C, Dubois J, Mathieu V, Kornienko A, Kiss R, Evidente, A. Lycorine, the Main Phenanthridine Amaryllidaceae Alkaloid, Exhibits Significant Antitumor Activity in Cancer Cells that Display Resistance to Proapoptotic Stimuli: An Investigation of Structure- Activity Relationship and Mechanistic Insight. J Med Chem. 2009; 52: 6244-6256.
• Şener B, Orhan İ, Satayavivad J. Antimalarial activity screening of some alkaloids and the plant extracts from Amaryllidaceae. Phytotherapy Res 2003; 17: 1220-1223.
• Lopez S, Bastida J, Viladomat F, Codina C. Acetylcholinestrase inhibitory activity of some Amaryllidaceae alkaloids and Narcissus extracts. Life Sciences 2002; 71: 2521-2529.
• Heinrich M, Galanthamine from Galanthus and other Amaryllidaceae-chemistry and biology based on traditional use. In: The Alkaloids Chemistry and Biology. Editor: Geoffrey Alan Cordell, Elsevier Inc., San Diego. 2010, vol. 68, pp. 157-165.
• European Pharmacopoeia, Sixth Edition. 2007, pp. 1261- 1266.
• Rhee IK, Van de Meent M, Ingkaninan K, Verpoorte R. Screening for acetylcholinesterase inhibitors from Amaryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining. J Chromatogr A 2001; 915: 217-223.
• Ünver-Somer N, Kaya G I, Sarıkaya B, Önür M A. Phytochemical studies on Galanthus woronowii Losinsk. 17th Meeting on Plant Originated Crude Drugs, Kuşadası, Abstract Book, p. 87, 2007.
• Kaya Gİ, Gözler T. Quantitative evaluation of the Specimens of Galanthus elwesii. J Fac Pharm Gazi 2003; 20: 71-78.
• Fillik A. Some Pharmacognostical Studies on Galanthus gracilis Ćelak. Growing in Mount Nif, Kemalpaşa, İzmir. MSc Thesis, Institution of Health Sciences, Ege University, İzmir, 2002.
• Kaya GI, Gözler B. Quantitative and cytotoxic activity determinations on Galanthus nivalis subsp. cilicicus. Fitoterapia 2005;76: 340-343.
• Akbulut İ. Some Pharmacognostical Studies on Galanthus plicatus Bieb. subsp. byzantinus (Baker) D.A. Webb Growing Around Abant Lake, Bolu. MSc Thesis, Institution of Health Sciences, Ege University, İzmir, 2004.
• Areshkina L. Ya. Alkaloids from snowdrop. Sovet Subtropiki. 1940; (11-12): 59. From: Khim Referat Zhur 1941; 4: 100 Chem. Abstr. (1943), 37: 64056.
• Kovtun L, Patudin AV, Gorbunova GM, Gorbunov VD, Stikhin VA, Gogitidze SD, Nakaidze AK. Search for galanthamine in Galanthus L. and Leucojum L. Plants in Transcaucasia, Farmatsevtichnii Zhurnal (Kiev) 1978; 6: 59-62; Chem. Abstr. (1979), 90: 200291r.
• Houghton PJ, Ren Y, Jayne-Howes M. Acetylcholinesterase inhibitors from plants and fungi. Nat Prod Rep 2006; 23: 181-199.
• Berkov S, Bastida J, Nikoleva M, Viladomat F, Codina C. Rapid TLC/GC-MS identification of acetylcholinesterase inhibitors in alkaloid extracts. Phytochem Anal 2008; 19: 411-419.
• Sarıkaya B, Kaya Gİ, Ünver-Somer N, Önür MA. Investigation of Galanthus woronowii with regard to acetylcholinesterase inhibitory activity. 18th Meeting on Plant Originated Crude Drugs, İstanbul, Fitomed Special Issue 2008; 6: 69.
• Ünver Somer N, Sarıkaya B, Kaya Gİ, Önür MA, Bastida J. Isolation and structure elucidation studies on Galanthus woronowii Losinsk. 9th International Symposium on Pharmaceutical Sciences, Ankara, Proceeding and Abstract Book, p. 329, 2009.