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Oxidative alterations during human platelet storage

Year 2011, Volume: 15 Issue: 1, 38 - 42, 06.03.2014

Bahar Göker Derya Özsavcı , Azize Şener Halil Aksoy , Vedat Bağışgil Gülderen Yanıkkaya Demirel Fikriye Uras

Abstract

Keywords

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References

  • Thon JN, Schubert P, Devine DV. Platelet storage lesion: a new understanding from a proteomic perspective. Transfus Med Rev, 22:268-79, 2008.
  • Eva M. Martın V, Jesus SY, Marina CR, Francisco PG, Marcial L. Comparasion between in vitro lipid peroxida- tion in fresh sheep platelets and peroxidative processes during sheep platelet ageing under storage at 4°C. Bio- chimica et Biophysica Acta, 1419: 313-324,1999.
  • Ozsavci D, Yardimci T, Demirel GY, Demiralp E, Uras F, Onder E. Flow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia. Platelets, 13:223-229, 2002.
  • PT Burch, JW Burch. Alterations in glutathione during storage of human platelet concentrates. Transfusion, 27:342-46, 1986.
  • Matsubayatsi E, Weidner J, Miraglia CC, Mcintyre JA. Platelet membrane early activation markers during pro- longed storage. Thromb Res, 93:151-60, 1999.
  • Jane E. F, Joseph L, Marc RB, Caroline a, John FK, Alan DM. Nitric oxide rleased from activated platelets inhibits platelet recruitment. J Clin Investigation, 2:350-56, 1997.
  • Sener A, Ozsavcı D, Oba R, Demirel YG, Uras F, Yardımcı KT.. Do platelet apoptosis, activation, aggre- gation, lipid peroxidation and platelet-leukocyte aggre- gate formation occur simultaneously in hyperlipidemia? Clinical Biochemistry, 38: 1081-1087, 2005.
  • Warshaw A.L., Laster L., Shulman N.R.: Protein synthe- sis by human platelets. J Biol Chem, 242:2094-7,1967.
  • Yardımcı T.U.: Membrane transport systems in human platelets. Hematologica, 65: 498-508,1980.
  • Brown, S.B., Clarke, M.C., Magowan, L., Sanderson, H. & Savill, J. Constitutive death of platelets leading to scav- enger receptor-mediated phagocytosis. A caspase inde- pendent cell clearance program. Journal of Biological Chemistry, 275: 5987–5996, 2000.
  • Tonon G, Lua X, Greco NJ, Chen W, Shi Y, Jamiesan GA.. Weak platlet agonist and U46619 induce apoptosis-like events in platelets, in the absence of phosphatidlyserine exposure. Thromb Res, 15: 425-50, 2002.
  • Rand ML, Wang H, Bang KWA, Poon KSV, Packham MA, Freedman J. Procoagulant surface exposure and apopto- sis in rabbit platelets: association with shortened survival and steady state. J Thromb Haemost, 2: 651-59, 2003.
  • Lowry O.H., Rosebrough N.J., Farr A.L, Randall R.H. Protein measurement with the folin phenol reagent. J Biol Chem, 193:265.1951.
  • Mergel, D. C., Anderman, G. Simultaneous determina- tion of oxidized and reduced glutathione in human rab- bit red cells. Methods Find. Exp. Clin. Pharmacol, 1:277- 283, 1979.
  • Buege, J. A., Aust, S. D. Microsomal lipid peroxidation. Methods Enzymol, 52:302-310, 1978.
  • Chen LY, Mehta P, Mehta JL. Oxidized LDL decreases L- arginine uptake and nitric oxide synthase protein expres- sion on human platelets. Circulation, 93:1740-6, 1996.
  • Azize Sener, Derya Ozsavci, Ozlem Bingol-Ozakpi- nar, Ozge Cevik, Gulderen Yanikkaya-Demirel, Turay Yardimci. Oxidized-LDL and Fe3+/ascorbic acid-in- duced oxidative modifications and phosphatidylserine exposure in human platelets are reduced by melatonin. Folia-Biologica, 55:45-52, 2009.
  • van der Wal DE, Du VX, Lo KS, Rasmussen JT, Verhoef S, Akkerman JW. Platelet apoptosis by cold-induced glycoprotein Ibα clustering. J Thromb Haemost, 23:2554- 2562, 2010.
  • Jackson SP, Schoenwaelder SM. Procoagulant platelets: are they necrotic? Blood, 116:2011-8, 2010.
  • George J.N.: Platelet membrane glycoproteins: alteration during storage of human platelet concantrates. Thromb Res, 8:719-724,1976.
  • Lentz BR. Exposure of platelet membrane phosphati- dylserine regulates blood coagulation. Prog Lipid Res, 42:423-38, 2003.
  • Shattil SJ, Cunningham M, Hoxie JA. Detection of acti- vated platelets in whole blood using activation-depend- ent monoclonal antibodies and flow cytometry. Blood, 70:307-15, 1987
  • van der Zee PM, Biró E, Ko Y, de Winter RJ, Hack CE, Sturk A, Nieuwland R. P-selectin- and CD63-exposing platelet microparticles reflect platelet activation in pe- ripheral arterial disease and myocardial infarction. Clin Chem, 52:657-64, 2006.
  • Dittmann J, Riggert J, Wieding JU, Simson G, Köhler M. Platelet membrane glycoproteins in thrombocytapher- esis and platelet concentrates. Beitr Infusionsther Trans- fusionsmed, 32:422-7, 1994
  • Perrotta PL, Perrotta CL, Snyder EL. Apoptotic activity in stored human platelets. Transfusion, 43:526-35, 2003.
  • Metcalfe P, Williamson L.M, Reutelingsperger C.P.M, Swann I, Ouwehand W.H, Goodall A.H. Activation dur- ing preparation of therapeutic platelets affects determi- nation during storage: a comparative flow cytometric study of different production methods. British Journal of Haematology, 98:86-95,1997.
  • Williams RH, Nollert MU. Platelet-derived NO slows thrombus growth on a collagen type III surface. Thromb J, 2:11-20, 2004.

Oxidative alterations during human platelet storage

Year 2011, Volume: 15 Issue: 1, 38 - 42, 06.03.2014

Bahar Göker Derya Özsavcı , Azize Şener Halil Aksoy , Vedat Bağışgil Gülderen Yanıkkaya Demirel Fikriye Uras

Abstract

Transfüzyon için aferezle elde edilen trombositlerde trombosit cevabında depolama sırasında çeşitli değişiklikler oluşur. Bu çalışmanın amacı depolamanın trombosit aktivasyonu, apoptozu, protein içeriği, lipid peroksidasyonu, nitrik oksit (NO) ve glutatyon (GSH) seviyelerine etkisini incelemektir. Bu çalışmada sağlıklı vericilerden aferezle elde edilen trombositler çalkalayıcıda 20-24°C’de 9 gün saklandı.1.,3.,5.,9. günlerde trombositler toplandı ve çöktürüldü. Trombosit aktivasyonu PAC-1 ve CD62-P antikorları ile, trombosit apoptozu ise Annexin-V ile flovsitometrede ölçüldü.Trombositler dört kez eritip donduruldu ve sonra NO, GSH ve malondialdehit (MDA) seviyeleri spektrofotometre ile tayin edildi. Protein dağılımı sodyum dodesil sülfat poliakrilamid jel elektroforezi (SDS-PAGE) ile araştırıldı. 1. günle karşılaştırıldığında, CD62-P, PAC-1 ekspresyonları ve Annexin-V seviyeleri 3.,5., ve 9., günlerde anlamlı düzeyde arttı. 1. günle karşılaştırıldığında trombosit NO ve GSH seviyeleri 3., 5., ve 9. günlerde azaldı, bununla birlikte MDA seviyeleri sadece 5. ve 9. günlerde anlamlı düzeyde arttı. Trombosit protein bandlarında hafif değişiklikler gözlendi. Bizim sonuçlarımız transfüzyon için hazırlanan trombositlerde trombosit aktivitesindeki değişikliklerin ilerde in vivo dolaşımda prokoagulant aktiviteyi ve böylece trombojenik riski artırabileceğini göstermektedir. Bu nedenle transfüzyon için taze örneklerin kullanılması ya da trombositlerin iyi korunması in vivo durumdaki trombosit davranışı için çok önemlidir

Keywords

ANAHTAR KELİMELER: Transfüzyon trombosit aktivasyon apoptoz protein

References

  • Thon JN, Schubert P, Devine DV. Platelet storage lesion: a new understanding from a proteomic perspective. Transfus Med Rev, 22:268-79, 2008.
  • Eva M. Martın V, Jesus SY, Marina CR, Francisco PG, Marcial L. Comparasion between in vitro lipid peroxida- tion in fresh sheep platelets and peroxidative processes during sheep platelet ageing under storage at 4°C. Bio- chimica et Biophysica Acta, 1419: 313-324,1999.
  • Ozsavci D, Yardimci T, Demirel GY, Demiralp E, Uras F, Onder E. Flow cytometric assay of platelet glycoprotein receptor numbers in hypercholesterolemia. Platelets, 13:223-229, 2002.
  • PT Burch, JW Burch. Alterations in glutathione during storage of human platelet concentrates. Transfusion, 27:342-46, 1986.
  • Matsubayatsi E, Weidner J, Miraglia CC, Mcintyre JA. Platelet membrane early activation markers during pro- longed storage. Thromb Res, 93:151-60, 1999.
  • Jane E. F, Joseph L, Marc RB, Caroline a, John FK, Alan DM. Nitric oxide rleased from activated platelets inhibits platelet recruitment. J Clin Investigation, 2:350-56, 1997.
  • Sener A, Ozsavcı D, Oba R, Demirel YG, Uras F, Yardımcı KT.. Do platelet apoptosis, activation, aggre- gation, lipid peroxidation and platelet-leukocyte aggre- gate formation occur simultaneously in hyperlipidemia? Clinical Biochemistry, 38: 1081-1087, 2005.
  • Warshaw A.L., Laster L., Shulman N.R.: Protein synthe- sis by human platelets. J Biol Chem, 242:2094-7,1967.
  • Yardımcı T.U.: Membrane transport systems in human platelets. Hematologica, 65: 498-508,1980.
  • Brown, S.B., Clarke, M.C., Magowan, L., Sanderson, H. & Savill, J. Constitutive death of platelets leading to scav- enger receptor-mediated phagocytosis. A caspase inde- pendent cell clearance program. Journal of Biological Chemistry, 275: 5987–5996, 2000.
  • Tonon G, Lua X, Greco NJ, Chen W, Shi Y, Jamiesan GA.. Weak platlet agonist and U46619 induce apoptosis-like events in platelets, in the absence of phosphatidlyserine exposure. Thromb Res, 15: 425-50, 2002.
  • Rand ML, Wang H, Bang KWA, Poon KSV, Packham MA, Freedman J. Procoagulant surface exposure and apopto- sis in rabbit platelets: association with shortened survival and steady state. J Thromb Haemost, 2: 651-59, 2003.
  • Lowry O.H., Rosebrough N.J., Farr A.L, Randall R.H. Protein measurement with the folin phenol reagent. J Biol Chem, 193:265.1951.
  • Mergel, D. C., Anderman, G. Simultaneous determina- tion of oxidized and reduced glutathione in human rab- bit red cells. Methods Find. Exp. Clin. Pharmacol, 1:277- 283, 1979.
  • Buege, J. A., Aust, S. D. Microsomal lipid peroxidation. Methods Enzymol, 52:302-310, 1978.
  • Chen LY, Mehta P, Mehta JL. Oxidized LDL decreases L- arginine uptake and nitric oxide synthase protein expres- sion on human platelets. Circulation, 93:1740-6, 1996.
  • Azize Sener, Derya Ozsavci, Ozlem Bingol-Ozakpi- nar, Ozge Cevik, Gulderen Yanikkaya-Demirel, Turay Yardimci. Oxidized-LDL and Fe3+/ascorbic acid-in- duced oxidative modifications and phosphatidylserine exposure in human platelets are reduced by melatonin. Folia-Biologica, 55:45-52, 2009.
  • van der Wal DE, Du VX, Lo KS, Rasmussen JT, Verhoef S, Akkerman JW. Platelet apoptosis by cold-induced glycoprotein Ibα clustering. J Thromb Haemost, 23:2554- 2562, 2010.
  • Jackson SP, Schoenwaelder SM. Procoagulant platelets: are they necrotic? Blood, 116:2011-8, 2010.
  • George J.N.: Platelet membrane glycoproteins: alteration during storage of human platelet concantrates. Thromb Res, 8:719-724,1976.
  • Lentz BR. Exposure of platelet membrane phosphati- dylserine regulates blood coagulation. Prog Lipid Res, 42:423-38, 2003.
  • Shattil SJ, Cunningham M, Hoxie JA. Detection of acti- vated platelets in whole blood using activation-depend- ent monoclonal antibodies and flow cytometry. Blood, 70:307-15, 1987
  • van der Zee PM, Biró E, Ko Y, de Winter RJ, Hack CE, Sturk A, Nieuwland R. P-selectin- and CD63-exposing platelet microparticles reflect platelet activation in pe- ripheral arterial disease and myocardial infarction. Clin Chem, 52:657-64, 2006.
  • Dittmann J, Riggert J, Wieding JU, Simson G, Köhler M. Platelet membrane glycoproteins in thrombocytapher- esis and platelet concentrates. Beitr Infusionsther Trans- fusionsmed, 32:422-7, 1994
  • Perrotta PL, Perrotta CL, Snyder EL. Apoptotic activity in stored human platelets. Transfusion, 43:526-35, 2003.
  • Metcalfe P, Williamson L.M, Reutelingsperger C.P.M, Swann I, Ouwehand W.H, Goodall A.H. Activation dur- ing preparation of therapeutic platelets affects determi- nation during storage: a comparative flow cytometric study of different production methods. British Journal of Haematology, 98:86-95,1997.
  • Williams RH, Nollert MU. Platelet-derived NO slows thrombus growth on a collagen type III surface. Thromb J, 2:11-20, 2004.
There are 27 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Bahar Göker This is me

Derya Özsavcı

Azize Şener This is me

Halil Aksoy

Vedat Bağışgil This is me

Gülderen Yanıkkaya Demirel This is me

Fikriye Uras This is me

Publication Date March 6, 2014
Published in Issue Year 2011 Volume: 15 Issue: 1

Cite

APA Göker, B., Özsavcı, D., Şener, A., Aksoy, H., et al. (2014). Oxidative alterations during human platelet storage. Marmara Pharmaceutical Journal, 15(1), 38-42. https://doi.org/10.12991/mpj.43619
AMA Göker B, Özsavcı D, Şener A, Aksoy H, Bağışgil V, Yanıkkaya Demirel G, Uras F. Oxidative alterations during human platelet storage. Marmara Pharm J. March 2014;15(1):38-42. doi:10.12991/mpj.43619
Chicago Göker, Bahar, Derya Özsavcı, Azize Şener, Halil Aksoy, Vedat Bağışgil, Gülderen Yanıkkaya Demirel, and Fikriye Uras. “Oxidative Alterations During Human Platelet Storage”. Marmara Pharmaceutical Journal 15, no. 1 (March 2014): 38-42. https://doi.org/10.12991/mpj.43619.
EndNote Göker B, Özsavcı D, Şener A, Aksoy H, Bağışgil V, Yanıkkaya Demirel G, Uras F (March 1, 2014) Oxidative alterations during human platelet storage. Marmara Pharmaceutical Journal 15 1 38–42.
IEEE B. Göker, D. Özsavcı, A. Şener, H. Aksoy, V. Bağışgil, G. Yanıkkaya Demirel, and F. Uras, “Oxidative alterations during human platelet storage”, Marmara Pharm J, vol. 15, no. 1, pp. 38–42, 2014, doi: 10.12991/mpj.43619.
ISNAD Göker, Bahar et al. “Oxidative Alterations During Human Platelet Storage”. Marmara Pharmaceutical Journal 15/1 (March 2014), 38-42. https://doi.org/10.12991/mpj.43619.
JAMA Göker B, Özsavcı D, Şener A, Aksoy H, Bağışgil V, Yanıkkaya Demirel G, Uras F. Oxidative alterations during human platelet storage. Marmara Pharm J. 2014;15:38–42.
MLA Göker, Bahar et al. “Oxidative Alterations During Human Platelet Storage”. Marmara Pharmaceutical Journal, vol. 15, no. 1, 2014, pp. 38-42, doi:10.12991/mpj.43619.
Vancouver Göker B, Özsavcı D, Şener A, Aksoy H, Bağışgil V, Yanıkkaya Demirel G, Uras F. Oxidative alterations during human platelet storage. Marmara Pharm J. 2014;15(1):38-42.