Abstract
Disorders of hemostatic mechanism play a role in the
development of diabetic complications and cardiovascular
events. C. albicans infection is frequently found in diabetic
individuals compared to nondiabetic individuals. In this
study, the in vitro effect of Candida albicans on the tissue
factor activity of the PMNS, MNS and platelet cells, that
were isolated from the blood samples of healthy and
diabetic individuals, was determined. The isolated blood
cells were counted after the viability test has been checked.
C.albicans suspension was added to one part of the cells.
An equal volume phosphate buffer was added to other part
of the cells instead of C.albicans suspension. Tissue factor
activity of all blood cells was determined by the method of
Quick. Tissue factor activity of these cells was found to be
significantly changed in the presence of C.albicans. The
presence of diabetes was also significantly changed tissue
factor activity of the PMNs, MNs and platelet cells when
compared to the healthy individuals. The findings of this
study will contribute to the treatment of diabetic patients
with or without candida infection in terms of tissue factor
activity.
Keywords: Tissue factor activity, diabetes, Candida
albicans, leukocyte, platelet
References
- Hudson BI, Hofmann MA, Bucciarelli L, Wendt T, Moser B, Lu Y, Qu W, Stern DM, D’Agati V, Yan SD, Yan SF, Grant PJ, Schmidt AM. Glycation and diabetes: the RAGE connection. Curr Sci 2002;83: 1515–21
- Geerlings SE, Hoepelman AI. Immune dysfunction in patients with diabetes mellitus (DM). FEMS Immunol Med Microbiol 1999;26:259-65.
- Odds FC. Candida and Candidiasis A Review and Biblography. Bailliere Tindall, London, 1988.
- Darwazeh AM, Lamey PJ, Samaranayake LP, MacFarlane TW, Fisher BM, Macrury SM, MacCuish AC. The relationship between colonisation secretor and in vitro adhesion of Candida albicans to buccalepithelial cells from diabetics. J Med Microbiol 1990;33:43-9.
- Al-Attas SA, Amro SO. Candidal colonization, strain diversity, and antifungal susceptibility among adult diabetic patients. Ann Saudi Med. 2010;30:101-8.
- Gerrits AJ, Koekman CA, van Haeften TW, Akkerman JWN. Platelet Tissue Factor Synthesis in Type 2 Diabetic Patients Is Resistant to Inhibition by Insulin. Diabetes 2010;59:1487-95.
- Ferreira IA, Mocking AI, Feijge MA, Gorter G, van Haeften TW, Heemskerk JW, Akkerman JW. Platelet inhibition by insulin is absent in type 2 diabetes mellitus. Arterioscler Thromb Vasc Biol 2006;26:417-22.
- Sommeijer DW, Hansen HR, van Oerle R, Hamulyak K, van Zanten AP, Meesters E, Spronk HM, ten Cate H. Soluble tissue factor is a candidate marker for progression of microvascular disease in patients with Type 2 diabetes. J Thromb Haemost 2006;4:574-80.
- Boden G, Vaidyula VR, Homko C, Cheung P, Rao AK. Circulating tissue factor procoagulant activity and thrombin
Abstract
References
- Hudson BI, Hofmann MA, Bucciarelli L, Wendt T, Moser B, Lu Y, Qu W, Stern DM, D’Agati V, Yan SD, Yan SF, Grant PJ, Schmidt AM. Glycation and diabetes: the RAGE connection. Curr Sci 2002;83: 1515–21
- Geerlings SE, Hoepelman AI. Immune dysfunction in patients with diabetes mellitus (DM). FEMS Immunol Med Microbiol 1999;26:259-65.
- Odds FC. Candida and Candidiasis A Review and Biblography. Bailliere Tindall, London, 1988.
- Darwazeh AM, Lamey PJ, Samaranayake LP, MacFarlane TW, Fisher BM, Macrury SM, MacCuish AC. The relationship between colonisation secretor and in vitro adhesion of Candida albicans to buccalepithelial cells from diabetics. J Med Microbiol 1990;33:43-9.
- Al-Attas SA, Amro SO. Candidal colonization, strain diversity, and antifungal susceptibility among adult diabetic patients. Ann Saudi Med. 2010;30:101-8.
- Gerrits AJ, Koekman CA, van Haeften TW, Akkerman JWN. Platelet Tissue Factor Synthesis in Type 2 Diabetic Patients Is Resistant to Inhibition by Insulin. Diabetes 2010;59:1487-95.
- Ferreira IA, Mocking AI, Feijge MA, Gorter G, van Haeften TW, Heemskerk JW, Akkerman JW. Platelet inhibition by insulin is absent in type 2 diabetes mellitus. Arterioscler Thromb Vasc Biol 2006;26:417-22.
- Sommeijer DW, Hansen HR, van Oerle R, Hamulyak K, van Zanten AP, Meesters E, Spronk HM, ten Cate H. Soluble tissue factor is a candidate marker for progression of microvascular disease in patients with Type 2 diabetes. J Thromb Haemost 2006;4:574-80.
- Boden G, Vaidyula VR, Homko C, Cheung P, Rao AK. Circulating tissue factor procoagulant activity and thrombin
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | January 7, 2015 |
| Published in Issue | Year 2015 Volume: 19 Issue: 1 |