Development and Validation of an Hplc Method for Voriconazole Active Substance in Bulk and its Pharmaceutical Formulation

Year 2016, Volume: 20 Issue: 2, 79 - 85, 02.02.2016

Neslihan Üstündağ Okur , Emre Şefik Çağlar Vildan Yozgatlı

Abstract

ABSTRACT

 

The aim of the present study was to develop and validate of voriconazole by using High-Performance Liquid Chromatography (HPLC). As mobile phase, mixture of acetonitrile and ultrapure water (50:50) (v/v) was used. The column was a C18 column (5µm,150x4,6mm). The eluent was monitored with UV detection at 256 nm at flow rate of 1 mL/min. The method was validated partially with respect to system suitability, linearity, limit of detection (LOD) and quantitation (LOQ), precision, accuracy and specificity, selectivity and stability. Obtained results were shown, the analytical method has good linearity, accuracy, precision, selectivity and stability. Analytical method development results indicated that the LOD was 0.02176 µg/mL; LOQ was 0.06528 µg/mL assay exhibited a linear range of 1- 30 mg/L.

Keywords

Voriconazole, HPlC, method, validation

Development and Validation of an Hplc Method for Voriconazole Active Substance in Bulk and its pharmaceutical Formulation

Abstract

References

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