Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines

Mohan Sumithra; V Chitra; Radika Ramasamy

doi:10.12991/marupj.300349

Research Article

Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines

Year 2017, Volume: 21 Issue: 2, 269 - 277, 01.04.2017

Mohan Sumithra V Chitra Radika Ramasamy

https://doi.org/10.12991/marupj.300349

Abstract

Spectral Analysis of Sargassum ilicifolium (Turner) C. Agardh

and in vitro Anti-proliferative Study of its Ethanolic Extract and

Chloroform Fraction Against Colon Cancer (HT-29) and Lung

Cancer (A549) Cell Lines.

Current research in drug discovery from medicinal plants

involves the identification of safe and inexpensive lead molecule

towards diseases. Cancer is a second major national burden

leading to cause death. Reducing the nation’s cancer burden is

a great challenge to win the “War of cancer”. During the last

decades, numerous novel compounds have been found from

marine organisms with interesting pharmaceutical activities.

Hence, a novel brown algae, Sargassum ilicifolium (Turner)

C.Agardh belonging to Sargassaceae family has selected and

the study was aimed to investigate the in vitro anti-proliferative

activity of ethanolic extract and chloroform fraction of

Sargassum ilicifolium (Turner) C.Agardh. against colon cancer

(HT-29) and lung cancer (A549) cell lines. Extraction of seaweed

using 70% ethanol and fractionated with various solvents. Based

on phytochemical screening, chloroform fraction was selected,

then it was subjected to GC-MS analysis. The 70% ethanolic

exract and chloroform fraction were selected to in vitro MTT

cell line assay using colon cancer cell line (HT-29) and lung

cancer cell line (A549). The GC-MS analysis report confirmed

the presence of 21 compounds and in the the invitro assay,

IC50value showed the effective anti-proliferative activity against

colon and lung cancer cell lines. The results of the present study

proved the anti-proliferative effect of Sargassum ilicifolium.

Further, the active biomolecule in the fraction has to be isolated

and charecterized the formulated product subjected to in vivo

study to strengthen the anticancer activity.

Keywords

Antiproliferative effect, colon cancer, lung cancer, Sargassum ilicifolium, GC-MS analysis

References

1. Coseri S. Natural products and their analogues as efficientanticancer drugs. Mini Rev Med Chem 2009; 9:560-71.
2. Newman D. Natural products as leads to potential drugs: anold process or the new hope for drug discovery? J Med Chem2008; 51:2589-99.
3. Newman D, Cargg G, Snader K. Natural products as sourcesof new drugs over the period 1981-2002. J Nat Proud 2003;6:1022-37.
4. Prakash NS, Sundaram R, Mitra SK. In vitro and In vivo anticancer activity of Bacoside A from whole plant of Bacopamonnieiri (Linn). Am J Pharmacol Toxicol 2011;5:11-9.
5. Ruperez P, Saura-Calixto F. Dietary fiber and physiochemicalproperties of edible Spanish seaweeds. Eur Food Res Technol2001; 212:349-54.
6. Lahaye M, Kaeffer B. Seaweed dietary fibres: Structure, physicchemicaland biological properties relevant to intestinalphysiology. Sci Aliment 1997; 17:619-39.
7. Yan X, Nagata T, Fan X. Antioxidant activities in somecommon seaweeds. Plant Foods Hum Nutr 1998;52:253-62.
8. Bhaskar N, Miyashita K. Lipid composition of Padinatetratomatica brown seaweed of the west cost of India. IndianJ Fish 2005;52:263-8.
9. Sumithra M, Arunachalam G. Acute and subacute oral toxicitystudy of Sargassum ilicifolium Turner C. Agardh in rodents.Int J PharmTech Res 2014; 6: 821-8.
10. Natarajan S,Shanmugiahthevar KP, Kasi PD. Cholinesteraseinhibitors from Sargassum and Gracilaria gracilis: Seaweedsinhibiting south Indian costal areas aslant, Gulf of Mannar.Nat Prod Res 2009;23:355-69.
11. Kang JY, Khan MN, Park NH, Cho JY, Lee MC, Fujii H, HongYK. Antipyretic, analgesic and anti-inflammatory activities ofseaweed Sagassum fulvellum and Sargassum thunbergi in mice.J Ethanopharmacol 2008;116:187-90.
12. Cartoni G, Ciardi M, Giarrusso A, Rosati F. Capillary gaschromatographic-mass spectrometric detection of anabolicsteroids. J Chromatogr 1983; 279:515–22.
13. Mossman T. Rapid colorimetric assay for cellular growth andsurvival:application to proliferation and cytotoxicity assays. JImmunol Method. 1983; 65:55-63.
14. Lee KH, Xiao Z. Podophyllotoxin and analogs. In: Anticanceragents from natural products. Editors: Cragg GM, KingstonDGI, Newman DJ. CRC Press. 2005, pp. 71-88

Sargassum ilicifolium (Turner) C.Agardh’ın Spektral Analizi ve Kolon Kanseri (HT-29) ve Akciğer Kanseri (A549) Hücre Serilerine Karşı Etanolik Ekstre ve Kloroform Fraksiyonunun in vitro Anti-proliferatif Çalışması

Year 2017, Volume: 21 Issue: 2, 269 - 277, 01.04.2017

Mohan Sumithra V Chitra Radika Ramasamy

https://doi.org/10.12991/marupj.300349

Abstract

Tıbbi bitkilerden ilaç keşfi ile ilgili güncel araştırmalar,

hastalıklara karşı güvenli ve ucuz öncü molekül tanımlanmasını

gerektirir. Kanser ölüme neden olan ikinci büyük ulusal bir

yüktür. “Kanser Savaşı” nı kazanmak için ulusun kanser

yükünü azaltmak, büyük bir mücadeledir. Son on yılda,

deniz organizmalarından ilginç farmasötik aktiviteleri ile çok

sayıda yeni bileşik bulunmuştur. Bu nedenle, bu çalışmada

Sargassaceae familyasına ait yeni bir kahverengi yosun olan

Sargassum ilicifolium (Turner) C.Agardh seçilmiş ve Sargassum

ilicifolium (Turner) C.Agardh’ın etanolik ekstraktı ve kloroform

fraksiyonunun kolon kanseri (HT-29) ve akciğer kanseri (A549)

hücre serilerine karşı in vitro anti-proliferatif etkinliğinin

araştırılması amaçlamıştır. Deniz yosununun ekstraksiyonu

%70 etanol ve çeşitli çözücüler kullanarak fraksiyonlanmıştır.

Fitokimyasal taramaya dayanılarak, kloroform fraksiyonu

seçildi, sonra GC-MS analizine tabi tutuldu. İn vitro MTT hücre

hattı deneyi için, kolon kanseri hücre hattı (HT-29) ve akciğer

kanseri hücre hattı (A549) kullanılarak % 70 etanolik ekstrakt

ve kloroform fraksiyonu seçildi. GC-MS analizi raporunda 21

bileşiğin varlığı doğrulandı ve in vitro kolon ve akciğer kanseri

hücre hatlarına karşı anti-proliferatif aktivite etkisi IC50

değeri ile gösterildi. İleride fraksiyondaki aktif biyomolekül

izole edilmeli ve antikanser aktivitesini güçlendirmek için in

vivo incelemeye tabi tutulan formüle edilmiş ürün karakterize

edilmelidir.

Keywords

Antiproliferatif etki, kolon kanseri, akciğer kanseri, Sargassum ilicifolium, GC-MS analizi

References

1. Coseri S. Natural products and their analogues as efficientanticancer drugs. Mini Rev Med Chem 2009; 9:560-71.
2. Newman D. Natural products as leads to potential drugs: anold process or the new hope for drug discovery? J Med Chem2008; 51:2589-99.
3. Newman D, Cargg G, Snader K. Natural products as sourcesof new drugs over the period 1981-2002. J Nat Proud 2003;6:1022-37.
4. Prakash NS, Sundaram R, Mitra SK. In vitro and In vivo anticancer activity of Bacoside A from whole plant of Bacopamonnieiri (Linn). Am J Pharmacol Toxicol 2011;5:11-9.
5. Ruperez P, Saura-Calixto F. Dietary fiber and physiochemicalproperties of edible Spanish seaweeds. Eur Food Res Technol2001; 212:349-54.
6. Lahaye M, Kaeffer B. Seaweed dietary fibres: Structure, physicchemicaland biological properties relevant to intestinalphysiology. Sci Aliment 1997; 17:619-39.
7. Yan X, Nagata T, Fan X. Antioxidant activities in somecommon seaweeds. Plant Foods Hum Nutr 1998;52:253-62.
8. Bhaskar N, Miyashita K. Lipid composition of Padinatetratomatica brown seaweed of the west cost of India. IndianJ Fish 2005;52:263-8.
9. Sumithra M, Arunachalam G. Acute and subacute oral toxicitystudy of Sargassum ilicifolium Turner C. Agardh in rodents.Int J PharmTech Res 2014; 6: 821-8.
10. Natarajan S,Shanmugiahthevar KP, Kasi PD. Cholinesteraseinhibitors from Sargassum and Gracilaria gracilis: Seaweedsinhibiting south Indian costal areas aslant, Gulf of Mannar.Nat Prod Res 2009;23:355-69.
11. Kang JY, Khan MN, Park NH, Cho JY, Lee MC, Fujii H, HongYK. Antipyretic, analgesic and anti-inflammatory activities ofseaweed Sagassum fulvellum and Sargassum thunbergi in mice.J Ethanopharmacol 2008;116:187-90.
12. Cartoni G, Ciardi M, Giarrusso A, Rosati F. Capillary gaschromatographic-mass spectrometric detection of anabolicsteroids. J Chromatogr 1983; 279:515–22.
13. Mossman T. Rapid colorimetric assay for cellular growth andsurvival:application to proliferation and cytotoxicity assays. JImmunol Method. 1983; 65:55-63.
14. Lee KH, Xiao Z. Podophyllotoxin and analogs. In: Anticanceragents from natural products. Editors: Cragg GM, KingstonDGI, Newman DJ. CRC Press. 2005, pp. 71-88

There are 14 citations in total.

Details

Subjects	Health Care Administration
Journal Section	Articles
Authors	Mohan Sumithra This is me V Chitra This is me Radika Ramasamy This is me
Publication Date	April 1, 2017
Published in Issue	Year 2017 Volume: 21 Issue: 2

Cite

APA	Sumithra, M., Chitra, V., & Ramasamy, R. (2017). Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines. Marmara Pharmaceutical Journal, 21(2), 269-277. https://doi.org/10.12991/marupj.300349
AMA	Sumithra M, Chitra V, Ramasamy R. Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines. J Res Pharm. May 2017;21(2):269-277. doi:10.12991/marupj.300349
Chicago	Sumithra, Mohan, V Chitra, and Radika Ramasamy. “Spectral Analysis of Sargassum Ilicifolium (Turner) C.Agardh and in Vitro Anti-Proliferative Study of Its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines”. Marmara Pharmaceutical Journal 21, no. 2 (May 2017): 269-77. https://doi.org/10.12991/marupj.300349.
EndNote	Sumithra M, Chitra V, Ramasamy R (May 1, 2017) Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines. Marmara Pharmaceutical Journal 21 2 269–277.
IEEE	M. Sumithra, V. Chitra, and R. Ramasamy, “Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines”, J Res Pharm, vol. 21, no. 2, pp. 269–277, 2017, doi: 10.12991/marupj.300349.
ISNAD	Sumithra, Mohan et al. “Spectral Analysis of Sargassum Ilicifolium (Turner) C.Agardh and in Vitro Anti-Proliferative Study of Its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines”. Marmara Pharmaceutical Journal 21/2 (May 2017), 269-277. https://doi.org/10.12991/marupj.300349.
JAMA	Sumithra M, Chitra V, Ramasamy R. Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines. J Res Pharm. 2017;21:269–277.
MLA	Sumithra, Mohan et al. “Spectral Analysis of Sargassum Ilicifolium (Turner) C.Agardh and in Vitro Anti-Proliferative Study of Its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines”. Marmara Pharmaceutical Journal, vol. 21, no. 2, 2017, pp. 269-77, doi:10.12991/marupj.300349.
Vancouver	Sumithra M, Chitra V, Ramasamy R. Spectral Analysis of Sargassum ilicifolium (Turner) C.Agardh and in vitro Anti-proliferative Study of its Ethanolic Extract and Chloroform Fraction Against Colon Cancer (HT-29) and Lung Cancer (A549) Cell Lines. J Res Pharm. 2017;21(2):269-77.