Research Article
Chemical Profile of Malva Neglecta and Malvella Sherardiana by Lc- MS/MS, GC/MS and Their Anticholinesterase, Antimicrobial and Antioxidant Properties With Aflatoxin-Contents
Abstract
The purpose of the present work was to calculate the phenolic
and the fatty acids profiles by LC-MS/MS and GC/MS with their
antioxidant, anticholinesterase and antimicrobial activities, and
aflatoxin contents of Malva neglecta and Malvella sherardiana.
The phenolic contents of M. neglecta and M. sherardiana were
determined by LC-MS/MS. Malic and 4-OH benzoic acids were
found to be the most abundant compounds in M. neglecta and M.
sherardiana, respectively. On the other hand, essential oils and
fatty acids composition were determined by GC/MS analysis.
The methanol extracts of the plants showed the highest effect
in all antioxidant assays of this study. The methanol extract of
M. neglecta showed the highest activity among the petroleum
ether, acetone, methanol and water extracts against acetyl- and
butyryl- cholinesterase enzymes (53.68% and 63.95% inhibition
ratio, respectively). The acetone extracts of M. neglacta and M.
sherardiana exhibited moderate activity against C. albicans with
18 and 17 mm inhibion zone diameter, respectively. The results
of the present study is also showed that the M. neglecta and M.
sherardiana can also be used as a food source due to its high
phenolic acid content and good antioxidant property. Also, the
samples were aflatoxin free.
References
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Guder A, Korkmaz H. Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iran J Pharm Res 2012;1: 913-23. 8. Dalar A, Türker M, Konczak I. Antioxidant capacity and phenolic constituents of Malva neglecta Wallr. and Plantago lanceolata L. from Eastern Anatolia Region of Turkey. J Herb Med 2012;2: 42-51. 9. Ghanati F, Khatami F. Polyphenols and their antioxidant activity in callus-cultured Malva Neglecta cells under UV-B and UV-C irradiation. Planta Med 2011;77: 1286-9. 10. Turker M, Dalar A. In vitro antioxidant and enzyme inhibitory properties and phenolic composition of M. neglecta Wallr. (Malvaceae) fruit: A traditional medicinal fruit from Eastern Anatolia. Ind Crop Prod 2013; 51: 376-80. 11. Barros L, Duenas M, Carvalho AM, Ferreira ICFR, Santos- Buelga C. Characterization of phenolic compounds in flowers of wild medicinal plants from Northeastern Portugal. Food Chem Toxicol 2012;50: 1576-82. 12. Daniela A, Pichichero E, Canuti L, Cicconi R, Karou D, D’arcangelo G, A Canini. Identification of phenolic compounds from medicinal and melliferous plants and their cytotoxic activity in cancer cells. Caryologia 2007;60: 90-5. 13. Khidyrova NK and Shakhidoyatov KHM. Plant polyprenols and their biological activity. Chem Nat Compd (Khim Prir Soedin) 2002;38: 107-21. 14. Ertaş A, Boğa M, Haşimi N, Yeşil Y, Gören AC, Topcu G, Kolak U. Antioxidant, anticholinesterase, antimicrobial activities and fatty acid constituents of Achillea cappadocica Hausskn. et Bornm. Turk J Chem 2014;38: 592-9. 15. Altun M, Gören AC. Essential Oil Composition of Satureja cuneifolia by simultaneous distillation-extraction and thermal 16. Ertaş A, Boğa M, Haşimi N, Yılmaz MA. Fatty acid and essential oil compositions of Trifolium angustifolium var. angustifolium with antioxidant, anticholinesterase and antimicrobial activities Iran J Pharm Res 2015;14: 233-4. 17. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Viticult 1977;28: 49-55. 18. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 2000;71: 109-14. 19. Miller HE. A simplified method for the evaluation of antioxidants. J Am Oil Chem Soc 1971;48: 91-105. 20. Apak R, Guclu K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J Agr Food Chem 2004;52: 7970-81. 21. Oran S, Sahin S, Sahinturk P, Ozturk S, Demir C. Antioxidant and antimicrobial potential and HPLC analysis of stictic and usnic acids of three Usnea species from Uludag Mountain (Bursa, Turkey) Iran J Pharm Res 2016;15: 527-35. 22. Delnavazi M, Hadjiakhoondi A, Delazar A, Ajani Y, S Tavakoli, Yassa N. Phytochemical and antioxidant ınvestigation of the aerial parts of Dorema glabrum Fisch. & C. A. Mey Iran J Pharm Res 2015;14: 925-31. 23. Nickavar B, Rezaee J, Nickavar A. Effect-directed analysis for the antioxidant compound in Salvia verticillata. Iran J Pharm Res 2016;15: 241-246. 24. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem Pharmacol 1961;7: 88-95. 25. Boğa M, Ertaş A, Yılmaz MA, Kızıl M, Çeken B, Haşimi N, Yılmaz Özden T, Demirci S, Yener İ, Deveci Ö. UHPLC-ESI-MS/MS and GC-MS analyses on phenolic, fatty acid and essential oil of Verbascum pinetorum with antioxidant, anticholinesterase, antimicrobial and DNA damage protection effects. Iran J Pharm Res 2016;15: 393-405. 26. NCCLS (National Committee for Clinical Laboratory Standards). Performance Standards for Antimicrobial Disk Susceptibility Test. 6th ed. Approved Standard M2-A6 Wayne Pa. 1997. 27. Ertaş A, Boğa M, Yılmaz MA, Yeşil Y, Haşimi N, Kaya MS, Temel H, Kolak U. Chemical compositions by using LC-MS/ MS and GC-MS and biological activities of Sedum sediforme (Jacq.) Pau. J Agr Food Chem 2014;62: 4601–9. 28. Ertaş A, Yilmaz MA, Firat M. Chemical profile by LC–MS/ MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Nat Prod Res 2015;29: 529-34. 29. Mousavi SNM, Delazar A, Nazemiyeh H, Khodaie I. Biological activity and phytochemical study of Scutellaria platystegia. Iran J Pharm Res 2015;14: 215-23. 30. Fahimi S, Mortazavi SA, Abdollahi M, Hajimehdipoor H. Formulation of a traditionally used polyherbal product for burn healing and HPTLC fingerprinting of its phenolic contents. Iran J Pharm Res 2016;15: 95-105. 31. Dowd MK. Identification of the unsaturated heptadecyl fatty acids in the seed oils of Thespesia populnea and Gossypium hirsutum J Am Oil Chem Soc 2012;89: 1599-1609. 32. Tesevic V, Vajs V, Lekic S, Dordevic I, Novakovic M, Vujisic L, Todosijevic M. Lipid composition and antioxidant activities of the seed oil from three Malvaceae species. Arch Biol Sci 2012;64: 221-7. 33. Kiliç CS, Aslan S, Kartal M, Coskun M. Fatty Acid Composition of Hibiscus trionum L. (Malvaceae). Rec Nat Prod 2011;5: 65- 9. 34. Vasudevan M, Parle M. Pharmacological actions of Thespesia populnea relevant to Alzheimer’s disease. Phytomedicine 2006;13: 677-87.
Chemical Profile of Malva Neglecta and Malvella Sherardiana by Lc- MS/MS, GC/MS and Their Anticholinesterase, Antimicrobial and Antioxidant Properties With Aflatoxin-Contents
Abstract
Bu çalışmanın amacı Malva neglecta ve Malvella sherardiana
türlerinin antioksidan, antikolinesteraz ve antimikrobiyal
aktiviterini, aflatoksin içeriği ile fenolik bileşik ve yağ asidi
profillerini LC-MS/MS ve GC/MS ile belirlemektir. İncelenen
yirmi yedi bileşikten, M. neglecta ve M. sherardiana türlerinde en
bol bulunan bileşiklerin sırasıyla malik (13108.97 μg/g) ve 4-OH
benzoik asit (2200.57 μg/g) olduğu saptanmıştır. M. neglecta
türünün metanol ekstresindeki toplam flavonoid içeriğin (15.58
μg QEs/mg ekstre) toplam fenolik içerikten (68.29 μg PEs/
mg ekstre) daha az olduğu belirlenmiştir. Bitkilerin metanol
ekstresi çalışılan antioksidan aktivite belirleme yöntemlerinin
tümünde en yüksek aktiviteyi göstermiştir. En iyi kolinesteraz
enzim inhibisyonu ise M. neglecta türünün metanol ekstresi
tarafından kaydedilmiştir (%53.68 AChE enzim inhibisyonu ve
%63.95 BChE enzim inhibisyonu). Bitkilerin aseton ekstreleri
ise C. albicans türüne karşı orta düzeyde antimikrobiyal
aktivite göstermiştir. Bu çalışmadan elde edilen sonuçlar, M.
neglecta ve M. sherardiana türlerinin yüksek fenolik asit içeriği
ve antioksidan aktivitesinden dolayı besin kaynağı olarak
kullanılabileceğini göstermektedir. Ayrıca ekstreler aflatoxin
içermemektedir.
References
- 1. Flora of Turkey and East Aegean Islands, Volume 2. Editor: Davis PH. Edinburgh University Press, Edinburgh. 1966, pp 179-242. 2. Akaydın G, Şimşek I, Arıtuluk ZC, Yeşilada E. An ethnobotanical survey in selected towns of the Mediterranean subregion (Turkey). Turk J Biol 2013;37: 230-47. 3. Yeşil Y, Akalın E. Folk medicinal plants in Kürecik Area (Akçadağ/Malatya-Turkey). Turk J Phar Sci 2009;2: 63-82. 4. Sezik E, Yaşilada E, Tabata M, Honda G, Takaishi Y, Fujita T. Traditional medicine in Turkey VIII. Folk medicine in East Anatolia, Erzurum, Erzincan, Area, Kars, Iğdır Provinces. Econ Bot 1997;51: 195-211. 5. Gurbuz I, Ozkan AM, Yesilada E, Kutsal O. Anti-ulcerogenic activity of some plants used in folk medicine of Pinarbasi (Kayseri, Turkey). J Ethnopharmacol 2005; 101: 313-8. 6. Mansouret SS, Koochak H, Darabpour E, Motamedi H. A survey on Hibiscus rosa-sinensis, Alcea rosea L. and Malva neglecta Wallr as antibacterial agents. Asian Pac J Trop Med 2010;3: 351-2. 7. Guder A, Korkmaz H. Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iran J Pharm Res 2012;1: 913-23. 8. Dalar A, Türker M, Konczak I. Antioxidant capacity and phenolic constituents of Malva neglecta Wallr. and Plantago lanceolata L. from Eastern Anatolia Region of Turkey. J Herb Med 2012;2: 42-51. 9. Ghanati F, Khatami F. Polyphenols and their antioxidant activity in callus-cultured Malva Neglecta cells under UV-B and UV-C irradiation. Planta Med 2011;77: 1286-9. 10. Turker M, Dalar A. In vitro antioxidant and enzyme inhibitory properties and phenolic composition of M. neglecta Wallr. (Malvaceae) fruit: A traditional medicinal fruit from Eastern Anatolia. Ind Crop Prod 2013; 51: 376-80. 11. Barros L, Duenas M, Carvalho AM, Ferreira ICFR, Santos- Buelga C. Characterization of phenolic compounds in flowers of wild medicinal plants from Northeastern Portugal. Food Chem Toxicol 2012;50: 1576-82. 12. Daniela A, Pichichero E, Canuti L, Cicconi R, Karou D, D’arcangelo G, A Canini. Identification of phenolic compounds from medicinal and melliferous plants and their cytotoxic activity in cancer cells. Caryologia 2007;60: 90-5. 13. Khidyrova NK and Shakhidoyatov KHM. Plant polyprenols and their biological activity. Chem Nat Compd (Khim Prir Soedin) 2002;38: 107-21. 14. Ertaş A, Boğa M, Haşimi N, Yeşil Y, Gören AC, Topcu G, Kolak U. Antioxidant, anticholinesterase, antimicrobial activities and fatty acid constituents of Achillea cappadocica Hausskn. et Bornm. Turk J Chem 2014;38: 592-9. 15. Altun M, Gören AC. Essential Oil Composition of Satureja cuneifolia by simultaneous distillation-extraction and thermal 16. Ertaş A, Boğa M, Haşimi N, Yılmaz MA. Fatty acid and essential oil compositions of Trifolium angustifolium var. angustifolium with antioxidant, anticholinesterase and antimicrobial activities Iran J Pharm Res 2015;14: 233-4. 17. Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Viticult 1977;28: 49-55. 18. Moreno MIN, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 2000;71: 109-14. 19. Miller HE. A simplified method for the evaluation of antioxidants. J Am Oil Chem Soc 1971;48: 91-105. 20. Apak R, Guclu K, Ozyurek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC Method. J Agr Food Chem 2004;52: 7970-81. 21. Oran S, Sahin S, Sahinturk P, Ozturk S, Demir C. Antioxidant and antimicrobial potential and HPLC analysis of stictic and usnic acids of three Usnea species from Uludag Mountain (Bursa, Turkey) Iran J Pharm Res 2016;15: 527-35. 22. Delnavazi M, Hadjiakhoondi A, Delazar A, Ajani Y, S Tavakoli, Yassa N. Phytochemical and antioxidant ınvestigation of the aerial parts of Dorema glabrum Fisch. & C. A. Mey Iran J Pharm Res 2015;14: 925-31. 23. Nickavar B, Rezaee J, Nickavar A. Effect-directed analysis for the antioxidant compound in Salvia verticillata. Iran J Pharm Res 2016;15: 241-246. 24. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem Pharmacol 1961;7: 88-95. 25. Boğa M, Ertaş A, Yılmaz MA, Kızıl M, Çeken B, Haşimi N, Yılmaz Özden T, Demirci S, Yener İ, Deveci Ö. UHPLC-ESI-MS/MS and GC-MS analyses on phenolic, fatty acid and essential oil of Verbascum pinetorum with antioxidant, anticholinesterase, antimicrobial and DNA damage protection effects. Iran J Pharm Res 2016;15: 393-405. 26. NCCLS (National Committee for Clinical Laboratory Standards). Performance Standards for Antimicrobial Disk Susceptibility Test. 6th ed. Approved Standard M2-A6 Wayne Pa. 1997. 27. Ertaş A, Boğa M, Yılmaz MA, Yeşil Y, Haşimi N, Kaya MS, Temel H, Kolak U. Chemical compositions by using LC-MS/ MS and GC-MS and biological activities of Sedum sediforme (Jacq.) Pau. J Agr Food Chem 2014;62: 4601–9. 28. Ertaş A, Yilmaz MA, Firat M. Chemical profile by LC–MS/ MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Nat Prod Res 2015;29: 529-34. 29. Mousavi SNM, Delazar A, Nazemiyeh H, Khodaie I. Biological activity and phytochemical study of Scutellaria platystegia. Iran J Pharm Res 2015;14: 215-23. 30. Fahimi S, Mortazavi SA, Abdollahi M, Hajimehdipoor H. Formulation of a traditionally used polyherbal product for burn healing and HPTLC fingerprinting of its phenolic contents. Iran J Pharm Res 2016;15: 95-105. 31. Dowd MK. Identification of the unsaturated heptadecyl fatty acids in the seed oils of Thespesia populnea and Gossypium hirsutum J Am Oil Chem Soc 2012;89: 1599-1609. 32. Tesevic V, Vajs V, Lekic S, Dordevic I, Novakovic M, Vujisic L, Todosijevic M. Lipid composition and antioxidant activities of the seed oil from three Malvaceae species. Arch Biol Sci 2012;64: 221-7. 33. Kiliç CS, Aslan S, Kartal M, Coskun M. Fatty Acid Composition of Hibiscus trionum L. (Malvaceae). Rec Nat Prod 2011;5: 65- 9. 34. Vasudevan M, Parle M. Pharmacological actions of Thespesia populnea relevant to Alzheimer’s disease. Phytomedicine 2006;13: 677-87.
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Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | April 21, 2017 |
| Published in Issue | Year 2017 Volume: 21 Issue: 3 |