Research Article
Year 2017,
Volume: 21 Issue: 3, 558 - 563, 20.06.2017
Abstract
Since last centuries, herbal medicines have been considered as
the main source for prevention and treatment a broad spectrum
of illnesses as well as infectious diseases. Here we evaluated the in
vivo activity of Berberis vulgaris methanolic root extract against
acute toxoplasmosis induced by Toxoplasma gondii, RH strain.
Male NMRI mice orally treated with B. vulgaris methanolic
extract at the dose of 1 and 2 g/kg once a day for 14 days after 24
h mice were infected intraperitoneally with 104 tachyzoites of T.
gondii, RH strain. The mortality rate in all infected mice and also
parasite load were determined to assess the efficacy B. vulgaris
methanolic extract against acute toxoplasmosis in mice model.
The obtained results demonstrated that the mortality rate of
infected mice was 100%, in 9th day after orally administration of
B. vulgaris at the concentrations of 1 and 2 g/kg; while all mice
in the control group were death in 5th day. Moreover obtained
findings revealed that the mean number of tachyzoites was
131×104 and 79×104 for infected mice treated with B. vulgaris
at the concentrations of 1 and 2 g/kg, respectively, whereas in
control group the mean number of tachyzoites was 288×104
parasite. The obtained findings demonstrated the potential
of B. vulgaris as a natural source for the production of new
prophylactic agent for use in toxoplasmosis.
References
- 1. Sukthana Y. Toxoplasmosis: Beyond animals to humans. Trends Parasitol 2006; 22: 137–42. 2. Mahmoudvand H, Sheibani V, Keshavarz H, Shojaee S, Esmaeelpour K, Ziaali N. Acetylcholinesterase inhibitor improves learning and memory impairment induced by Toxoplasma gondii infection. Iran J Parasitol 2016; 11: 177-85. 3. Biesiada G, Kalinowska-Nowak A, Czepiel J, March T. Toxoplasmosis—epidemiology, clinical manifestation and infection in pregnant women. Przegl Lek 2006; 63: 97–9. 4. Mahmoudvand H, Ziaali N, Ghazvini H, Shojaee S, Keshavarz H, Esmaeeilpour K, Sheibani V. Toxoplasma gondii infection promotes neuroinflamation through cytokine networks and induced hyperalgesia in BALB/c mice. Inflamation 2016; 39: 405-12. 5. Signorini L, Gulletta M, Coppini D, Donzelli C, Stellini R, Manca N, Carosi G, Matteelli A. Fatal disseminated toxoplasmosis during primary HIV infection. Curr HIV Res 2007; 5: 273-4. 6. Mahmoudvand H, Sheibani V, Shojaee S, Mirbadie SR, Keshavarz H, Esmaeelpour K, Keyhani AR, Ziaali N. Toxoplasma gondii Infection Potentiates Cognitive Impairments of Alzheimer’s Disease in the BALB/c Mice. J Parasitol 2016; 102: 629-35. 7. Tavakoli Kareshk A, Keyhani A, Asadi A, Zia-Ali N, Mahmoudvand H, Mohammadi AR. Seroprevalence of Toxoplasma gondii infection among childbearing age women in Kerman city, southeastern Iran. J Parasit Dis 2016; 40: 1544- 7. 8. Rocha LG, Almeida JR, Macedo RO, Barbosa-Filho JM. A review of natural products with antileishmanial activity. Phytomedicine 2005; 12: 514-35. 9. Zargari A. Medicinal Plants. Tehran University, Tehran 1996. 10. Imanshahidi H, Hosseinzadeh H. Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, Berberine. Phytother Res 2008; 22: 999-1012. 11. Mahmoudvand H, Asadi A, Harandi MF, Sharififar F, Jahanbakhsh S, Dezaki ES. In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces. Iran J Basic Med Sci 2014; 17:1001-6. 12. Asgari H, Keshavarz H, Shojaee S, Motazedian MH, Mohebali M, Miri R, Mehrabani D, Rezaeian M. In vitro and in vivo potential of RH strain of Toxoplasma gondii (Type Ι) in tissue cyst forming. Iranian J Parasitol 2013; 8: 367-75. 13. Tavakoli Kareshk A, Keyhani A, Mahmoudvand H, Tavakoli Oliaei R, Asadi A, Andishmand M, Azzizian H, Babaei Z, Zia- Ali N. Efficacy of the Bunium persicum (Boiss) essential oil against acute toxoplasmosis in mice model. Iran J Parasitol 2015; 10: 625-31. 14. Ivanovska N, Philipov S. Study of the antiinflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int J Immunopharmacol 1996;18: 553–61. 15. Ghaderi R, Maleki Nejad P. evaluation of anticandidal effects of Berberis vulgaris root extracts (methanolic and aqueous) and comparing their effects with those clotrimazole. J Birjand Univ Med Sci 2006; 13: 42-8. 16. Freile ML, Giannini F, Pucci G, Sturniolo A, Rodero L, Pucci O. Antimicrobial activity of aqueous extracts and of berberine isolated from Berberis heterophylla. Fitoterapia 2003; 74: 702- 5. 17. Mahmoudvand H, Ayatollahi Mousavi SA, Sepahvand A, Sharififar F, Ezatpour B, Gorohi F, Saedi Dezaki E, Jahanbakhsh S. Antifungal, antileishmanial, and cytotoxicity activities of various extracts of Berberis vulgaris (Berberidaceae) and its active principle berberine. ISRN Pharmacol 2014; 2014:602436. 18. Fata A, Rakhshandeh H, Berenji F, Jalalifard A. Treatment of cutaneous leishmaniasis in murine model by alcoholic extract of Berberis vulgaris. Iran J Parasitol 2006; 1: 39-42. 19. Kaneda Y, Torii M, Tanaka T, Aikawa M. In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis. Ann Trop Med Parasitol 1991; 85: 417-25. 20. Mahmoudvand H, Saedi Dezaki E, Sharififar F, Ezatpour B, Jahanbakhsh S, Fasihi Harandi M. Protoscolecidal effect of Berberis vulgaris root extract and its main compound,berberine in cystic echinococcosis. Iran J Parasitol 2014; 9:503-10. 21. Sheng WD, Jiddawi MS, Hong XQ, Abdulla SM. Treatment of chloroquine-resistant malaria using pyrimethamine in combination with berberine, tetracycline or cotrimoxazole. East Afr Med J 1997; 74: 283-4. 22. Vennerstrom JL, Lovelace JK, Waits VB, Hanson WL, Klayman DL. Berberine derivatives as anti-leishmanial drugs. Antimicrob Agent Chemother 2005; 34:198-211. 23. Peng L, Kang SH, Yin ZH, Jia R, Song X, Li L, Li Z, Zou Y, Liang X, Li L, He C, Ye G, Yin L, Shi F, Lv C, Jing B. Antibacterial activity and mechanism of berberine against Streptococcus agalactiae. Int J Clin Exp Pathol 2015; 8: 5217–23. 24. Peychev L. Pharmacological investigation on the cardiovascular effects of Berberis vulgaris on tested animals. Pharmacia 2005; 52: 118-21. 25. Mahmoudvand H, Sharififar F, Sharifi I, Ezatpour B, Fasihi Harandi M, Makki MS, Zia-Ali N, Jahanbakhsh S. In vitro inhibitory effect of Berberis vulgaris (Berberidaceae) and its main component, berberine against different Leishmania species. Iran J Parasitol 2014; 9:28-36.
Year 2017,
Volume: 21 Issue: 3, 558 - 563, 20.06.2017
Abstract
Son birkaç yüzyıldır bitkisel kaynaklardan elde edilen ilaçlar
çeşitli hastalıklarla birlikte enfeksiyon hastalıklarının da
tedavisinde ve profilaksisinde kullanılmaktadır. Bu çalışmada;
Berberis vulgaris’in köklerinden hazırlanan matanollü
ekstrenin, Toxoplasma gondii RH suşuyla enfekte edilerek
akut toksoplazmozis oluşturulmuş farelerdeki etkisi araştırıldı.
Erkek NMRI fareleri intraperitoneal yoldan Toxoplasma gondii
RH suşuna ait 104 taşizoitle enfekte edildikten 24 saat sonra
bu farelere oral yoldan Berberis vulgaris’in ekstresi 1 ve 2 g/kg
dozlarda, günde 1 kez, 14 gün boyunca uygulandı. Tüm farelerde
ölüm oranı ve parazit yükü tespit edilerek akut toksoplazmoziste
B. vulgaris’in metanollü ekstresinin etkisi belirlendi. Berberis
vulgaris’in 1 ve 2 g/kg dozlarda oral yoldan uygulanmasının
takip eden 9. günde farelerde ölüm oranı %100 olarak belirlendi,
kontrol grubundaki bütün farelerin 5.günün sonunda öldüğü
gözlendi. Berberis vulgaris’in 1 ve 2 g/kg dozlarda uygulandığı
gruplarda ortalama taşizoit değeri sırasıyla 131×104 ve 79×104
olarak saptanırken kontrol grubunda taşizoit sayısı ortalama
288×104 parazit olarak belirlendi. Elde edilen sonuçlar
dikkate alınarak B. vulgaris’in toksoplazmozis profilaksisinde
kullanılabilecek bir ilacın üretiminde doğal kaynaklı bir bileşen
olarak kullanılabilirliği gösterilmiştir.
References
- 1. Sukthana Y. Toxoplasmosis: Beyond animals to humans. Trends Parasitol 2006; 22: 137–42. 2. Mahmoudvand H, Sheibani V, Keshavarz H, Shojaee S, Esmaeelpour K, Ziaali N. Acetylcholinesterase inhibitor improves learning and memory impairment induced by Toxoplasma gondii infection. Iran J Parasitol 2016; 11: 177-85. 3. Biesiada G, Kalinowska-Nowak A, Czepiel J, March T. Toxoplasmosis—epidemiology, clinical manifestation and infection in pregnant women. Przegl Lek 2006; 63: 97–9. 4. Mahmoudvand H, Ziaali N, Ghazvini H, Shojaee S, Keshavarz H, Esmaeeilpour K, Sheibani V. Toxoplasma gondii infection promotes neuroinflamation through cytokine networks and induced hyperalgesia in BALB/c mice. Inflamation 2016; 39: 405-12. 5. Signorini L, Gulletta M, Coppini D, Donzelli C, Stellini R, Manca N, Carosi G, Matteelli A. Fatal disseminated toxoplasmosis during primary HIV infection. Curr HIV Res 2007; 5: 273-4. 6. Mahmoudvand H, Sheibani V, Shojaee S, Mirbadie SR, Keshavarz H, Esmaeelpour K, Keyhani AR, Ziaali N. Toxoplasma gondii Infection Potentiates Cognitive Impairments of Alzheimer’s Disease in the BALB/c Mice. J Parasitol 2016; 102: 629-35. 7. Tavakoli Kareshk A, Keyhani A, Asadi A, Zia-Ali N, Mahmoudvand H, Mohammadi AR. Seroprevalence of Toxoplasma gondii infection among childbearing age women in Kerman city, southeastern Iran. J Parasit Dis 2016; 40: 1544- 7. 8. Rocha LG, Almeida JR, Macedo RO, Barbosa-Filho JM. A review of natural products with antileishmanial activity. Phytomedicine 2005; 12: 514-35. 9. Zargari A. Medicinal Plants. Tehran University, Tehran 1996. 10. Imanshahidi H, Hosseinzadeh H. Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, Berberine. Phytother Res 2008; 22: 999-1012. 11. Mahmoudvand H, Asadi A, Harandi MF, Sharififar F, Jahanbakhsh S, Dezaki ES. In vitro lethal effects of various extracts of Nigella sativa seed on hydatid cyst protoscoleces. Iran J Basic Med Sci 2014; 17:1001-6. 12. Asgari H, Keshavarz H, Shojaee S, Motazedian MH, Mohebali M, Miri R, Mehrabani D, Rezaeian M. In vitro and in vivo potential of RH strain of Toxoplasma gondii (Type Ι) in tissue cyst forming. Iranian J Parasitol 2013; 8: 367-75. 13. Tavakoli Kareshk A, Keyhani A, Mahmoudvand H, Tavakoli Oliaei R, Asadi A, Andishmand M, Azzizian H, Babaei Z, Zia- Ali N. Efficacy of the Bunium persicum (Boiss) essential oil against acute toxoplasmosis in mice model. Iran J Parasitol 2015; 10: 625-31. 14. Ivanovska N, Philipov S. Study of the antiinflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int J Immunopharmacol 1996;18: 553–61. 15. Ghaderi R, Maleki Nejad P. evaluation of anticandidal effects of Berberis vulgaris root extracts (methanolic and aqueous) and comparing their effects with those clotrimazole. J Birjand Univ Med Sci 2006; 13: 42-8. 16. Freile ML, Giannini F, Pucci G, Sturniolo A, Rodero L, Pucci O. Antimicrobial activity of aqueous extracts and of berberine isolated from Berberis heterophylla. Fitoterapia 2003; 74: 702- 5. 17. Mahmoudvand H, Ayatollahi Mousavi SA, Sepahvand A, Sharififar F, Ezatpour B, Gorohi F, Saedi Dezaki E, Jahanbakhsh S. Antifungal, antileishmanial, and cytotoxicity activities of various extracts of Berberis vulgaris (Berberidaceae) and its active principle berberine. ISRN Pharmacol 2014; 2014:602436. 18. Fata A, Rakhshandeh H, Berenji F, Jalalifard A. Treatment of cutaneous leishmaniasis in murine model by alcoholic extract of Berberis vulgaris. Iran J Parasitol 2006; 1: 39-42. 19. Kaneda Y, Torii M, Tanaka T, Aikawa M. In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis. Ann Trop Med Parasitol 1991; 85: 417-25. 20. Mahmoudvand H, Saedi Dezaki E, Sharififar F, Ezatpour B, Jahanbakhsh S, Fasihi Harandi M. Protoscolecidal effect of Berberis vulgaris root extract and its main compound,berberine in cystic echinococcosis. Iran J Parasitol 2014; 9:503-10. 21. Sheng WD, Jiddawi MS, Hong XQ, Abdulla SM. Treatment of chloroquine-resistant malaria using pyrimethamine in combination with berberine, tetracycline or cotrimoxazole. East Afr Med J 1997; 74: 283-4. 22. Vennerstrom JL, Lovelace JK, Waits VB, Hanson WL, Klayman DL. Berberine derivatives as anti-leishmanial drugs. Antimicrob Agent Chemother 2005; 34:198-211. 23. Peng L, Kang SH, Yin ZH, Jia R, Song X, Li L, Li Z, Zou Y, Liang X, Li L, He C, Ye G, Yin L, Shi F, Lv C, Jing B. Antibacterial activity and mechanism of berberine against Streptococcus agalactiae. Int J Clin Exp Pathol 2015; 8: 5217–23. 24. Peychev L. Pharmacological investigation on the cardiovascular effects of Berberis vulgaris on tested animals. Pharmacia 2005; 52: 118-21. 25. Mahmoudvand H, Sharififar F, Sharifi I, Ezatpour B, Fasihi Harandi M, Makki MS, Zia-Ali N, Jahanbakhsh S. In vitro inhibitory effect of Berberis vulgaris (Berberidaceae) and its main component, berberine against different Leishmania species. Iran J Parasitol 2014; 9:28-36.
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Details
| Subjects | Health Care Administration |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | June 20, 2017 |
| Published in Issue | Year 2017 Volume: 21 Issue: 3 |