Central Composite Design to develop a robust RP-TLC/Densitometry method for quantification of Triclosan in cosmetic preparations

Year 2017, Volume: 21 Issue: 3, 612 - 619, 23.06.2017

Kavitha Jayaseelan Santhana Lakshmi Santhana Lakshmi

https://doi.org/10.12991/marupj.323289

Abstract

The current study was framed with a view to develop a

simple, sensitive and a rapid RP-TLC/Densitometry method

for quantification of Triclosan (TCS) in various cosmetic

preparations available in local market. Moreover, robustness

testing was planned to be performed employing Central

Composite Design (CCD). The desired chromatographic

separation was achieved on RP-TLC/Densitometry plates

precoated with Silica gel 60 GF254 on Aluminum sheet,

employing methanol : glacial acetic acid as mobile phase in

the ratio of 7 : 3 v/v and detection was carried out at 281 nm.

The mobile phase used gave an excellent symmetrical peak for

TCS. The method was found linear over a wide range of 20-140

ng/band. The optimized method was validated by measuring

various validation parameters. CCD was employed to check the

robustness of the method at three-factor levels. The developed

and optimized method was used to quantify TCS in 20 different

cosmetic preparations procured from local market. The

proposed and developed RP-TLC/Densitometry method can

be applied for routine analysis of TCS in cosmetic preparations

and can also be extended to the analysis in pharmaceuticals and

food products.

Keywords

Central Composite Design, Cosmetic preparations, Triclosan, RP-TLC/Densitometry, Validation

Kozmetik preparatların içeriğinde bulunan triklosanın tayini amacıyla güvenilir bir ters faz-ince tabaka kromatografisi/ densitometri yöntemi geliştirmek için merkezi bileşik tasarımın uygulanması

Abstract

Bu çalışmada, satışa sunulmuş kozmetik ürünlerde triklosan’ın

(TCS) miktar tayini amacıyla kullanıacak basit, hızlı ve duyarlı

bir ters faz-ince tabaka kromatografisi/densitometri yöntemi

geliştirildi. Geliştirilen yönteme ek olarak elde edilen sistemin

sağlamlığının kanıtlanması için yapılan analizlerde merkezi

bileşik tasarım (CCD) yöntemi uygulandı. Kromatografik ayrım

için silika jel 60 GF254 kaplı, ters faz-ince tabaka kromatografisi/

densitometri yöntemi için üretilmiş alüminyum plaklar ve

metanol : buzlu asetik 7:3 h/h karışımından oluşan hareketli

faz kullanıldı, deteksiyon dalga boyu 281 nm olarak belirlendi.

Kullanılan hareketli faz TCS için mükemmel simetriye sahip

piklerin elde edilmesini sağladı. Yöntem; 2 - 200 ng/band

gibi geniş bir aralık için doğrusal olarak tespit edildi. Birçok

validasyon parametresi incelenerek yöntem en iyi hale getirildi.

Sistemin sağlamlığının kontrolü için üç-faktör düzeyinde CCD

yöntemi kullanıldı. Geliştirilen ve en iyi hale getirilen yöntem

yerel satıcılardan edinilen 20 farklı kozmetik ürünün içeriğinde

bulunan triklosan’ın miktar tayiminin yapılması için kullanıldı.

Geliştirilen ters faz-ince tabaka kromatografisi/densitometri

yönteminin kozmetik ürünlerde triklosan’ın miktar tayini için

rutin olarak kulanılacağı gibi farmasötik ürünlerde ve gıdalarda

yapılacak aynı amaçlı analizlerde de kullanımı önerilmektedir.

Keywords

Merkezi Bileşik Tasarım, kozmetik ürünler, Triklosan, ters faz-ince tabaka kromatografisi/densitometri, Validasyon

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