In vitro anti influenza virus activity, antioxidant potential and total phenolic content of twelve Iranian medicinal plants

Year 2017, Volume: 21 Issue: 4, 843 - 851, 01.12.2017

Mohammad-Taghi Moradı Ali Karımı Zahra Lorıgooını Batoul Pourgheysarı Somayeh Alıdadı Leila Hashemı

Abstract

The emergence of medicine resistance strains of influenza

A viruses to the chemical drogs lead to the development of

alternative herbal compounds that inhibit the virus replication.

Therefore, the aim of this research was to investigate in vitro

anti-influenza A viruses activity, antioxidant potential, total

phenolic, and flavonoid content of a total of 12 hydro alcoholic

crude extracts obtained from 8 kinds of medicinal plants. Antiinfluenza

A viruses activity of the extracts was investigated by

the using of MDCK cell line and MTT (3-[4,5-dimethylthiazol–

2-yl]-2,5-diphenyltetrazolium bromide) method. Both

50% inhibitory concentration (IC50) and 50% cytotoxicity

concentration (CC50) of the extracts were identified using

regression analysis. The antioxidant activity, total phenol, and

flavonoid content of the extracts were determined using 2,

2-diphenyl-1-picrylhydrazyl (DPPH) assay, Folin-Ciocalteu

method, and aluminum chloride colorimetric method,

respectively. The results demonstrated that there was high

activity against influenza virus for Peganum harmala L.,

Equisetum arvense L., and Punica granatum L, extracts with IC50

value of 9.1 (CI95%:7.3-11.3), 6.45 (CI95%: 4.5-9.23), and 104.5

(CI95%: 82.8-131.8), respectively. DPPH radical scavenging

activity showed that both Equisetum arvense L. and Punica

granatum L. demonstrated the highest antioxidant activity with

IC50 value of 6.5, 6.8 and 7.7 μg/mL, respectively. According to

the results, some of these extracts might be further analyzed to

develop effective anti-influenza factors.

Keywords

Antiviral activity, medical plant, Influenza virus, Antioxidant potential

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