Anti-adenovirus activity, antioxidant potential, and phenolic content of dried flower buds of Syzygium aromaticum extract in HEp2 cell line

Year 2017, Volume: 21 Issue: 4, 852 - 859, 01.12.2017

Mohammad-Taghi Moradı Ali Karımı Somayeh Alıdadı Leila Hashemı

Abstract

The aim of this study is to evaluate in vitro anti-adenovirus

property, antioxidant potential, and total phenolic content

of dried flowers buds of Syzygium aromaticum crude extract.

The crud extract was prepared and its anti-adenovirus

activity was investigated on HEp2 cell line using MTT

(3-[4,5-dimethylthiazol–2-yl]-2,5-diphenyltetrazolium

bromide) assay. The 2,2-diphenyl-1-picrylhydrazyl (DPPH)

assay, Folin-Ciocalteu method and aluminum chloride

colorimetric method was used to determine antioxidant activity,

total phenol content, flavonoids and flavonols content of the

extract, respectively. Based on results, the 50% cytotoxicity

concentration (CC50) and the 50% inhibitory concentration

(IC50) of the extract were 97.66±11.4 and 4.73±1.6 μg/ml,

respectively, with the selectivity index (SI) of 20.64. The crud

extract inhibited adenovirus replication in post-adsorption step

(p<0.05). The extract showed remarkable scavenging activity

with IC50 values of 10.05±1.93 μg/ml. Total phenolic, flavonoid

and flavonol content of the crude extract was 255.8±3.95

mg GAE/g, 63.9±2.35 mg RUT/g and 62±2.35 mg RUT/g,

respectively. The results of the present study indicated that S.

aromaticum crude extract exhibited anti-adenovirus activity

with inhibitory effect on adenovirus replication

Keywords

Antiviral; clove; Syzygium aromaticum; Adenovirus; antioxidant activity, Phenolic content

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