Phytochemical composition and antifeedant activity of five Vincetoxicum taxa against Spodoptera littoralis and Leptinotarsa decemlineata

Year 2017, Volume: 21 Issue: 4, 872 - 880, 01.12.2017

Sevda Güzel Roman Pavela Ahmet İlçim Gamze Kökdil

Abstract

Five Vincetoxicum taxa (V. canescens subsp. canescens, V.

canescens subsp. pedunculata, V. fuscatum subsp. fuscatum, V.

fuscatum subsp. boissieri and V. parviflorum) were investigated

for insect antifeedant activity against Spodoptera littoralis and

Leptinotarsa decemlineata. At a dose of 500 μg/cm2, 12 extracts

were 100 % feeding deterrent, while 6 extracts showed 86.3-

99.3 % antifeedant activity. The effective doses (ED50) of the

total ethanol extract from V. canescens subsp. pedunculata

and of the methanol: dichloromethane (1:1) extract from V.

parviflorum were 12, and 18 μg/cm2, respectively, against S.

littoralis, and that of the total ethanol extract from V. fuscatum

subsp. fuscatum was 25 μg/cm2 against L. decemlineata. The

dichloromethane extracts of V. parviflorum and V. canescens

subsp. pedunculata inhibited the growth of S. littoralis with

ED50 values of 0.08 and 0.09 mg/g, respectively, and their LD50

values for larval mortality were 1.07 and 1.03 mg/g, respectively.

Phytochemical analysis revealed the presence of cardiotonic

glycosides, sugars, flavonoids and alkaloids in all investigated

taxa. In the dichloromethane and methanol:dichloromethane

(1:1) extracts, phenanthroindolizidine alkaloids were detected

by LC/MS/MS.

Keywords

Vincetoxicum; antifeedant activity; phytochemical composition; Spodoptera littoralis; Leptinotarsa decemlineata

References

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